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The map label for this gene is dhaK [H]

Identifier: 218547840

GI number: 218547840

Start: 440952

End: 442706

Strand: Direct

Name: dhaK [H]

Synonym: EFER_0430

Alternate gene names: 218547840

Gene position: 440952-442706 (Clockwise)

Preceding gene: 218547839

Following gene: 218547841

Centisome position: 9.61

GC content: 60.28

Gene sequence:

>1755_bases
ATGACATGGCTTTTTAATAATCCCTCCGACTTTGCCAAAGAGATGGTGGCGGGTTTCGTCAGCGCCAATGCTGACATCGT
GCGCCAGGTGCCGGGCGGGGTTATTCGCAATACCCAAAGTCAGCAAGGTACGGTGGCGATTATCATCGGCGGCGGCTCGG
GCCACTACCCGGCGTTTGCCGGGCTGGTCGGCCAGGGACTGGCCCACGGCGCGGCGATGGGAAACTTATTCGCCTCACCC
TCCGCGCAGCAAATTTGCTCGGTGGCGAAAGCCGCCAACAACGGGGCGGGCGTGCTGTTAGCGTTTGGCAACTACGCCGG
GGACGTTCTGCATTTTGGCCTCGCACGGGAGCGCCTGATTGCAGAAGGCATTCCCTGCGAACTTATCGCCGTTACTGATG
ACATCACCAGCGCCGGACAACATGAGCCCGAAAAACGCCGCGGCGTGGCGGGCGATTTAGTGGTGTTCAAAGCGGCTGCG
GCTGCGGCGGAGCAGGGGGCTTCACTGGAAGAGGTACTTACCGTCGCCAGACACGCCAACGATCGCACCCGTACCATCGG
GGTCGCCTTTTCTGGCTGCACATTGCCAGGGGCGGAGCAGCCTTTGTTTACCATTCCGCAAGGGCAAATGGGCTTTGGCA
TGGGGATCCACGGCGAACCGGGTATCCGTGAAATAGCCGTGCCGGGGGCCGATGAACTGGCGACAATGTTAGTTGAACAT
CTGCTCAACGATGTTCCATCTGGCGTGAGCATCAAAGGTGCCCGCGTTGGGGCGATCTTAAACGGTCTCGGCTCTGTGAA
ATATGAAGAACTTTTTGTAGTCTGGCACAGCGTGCAGCAACGGCTGAACGAGCAGGGCATTATCCTGGCTGATATTCAGG
TCGGCGAATTTGTCACCAGTTTCGATATGGCGGGCCTGTCACTGACGCTCATCTGGTTTAGCGAAGCGTTAGAGGCACAG
TGGCAGGCTCCGGCTTACACGTCGGCTTTCAGGCGTGGCGCGGTAATTGCCGCGCAGCCACGACAGGCGAGCAGTGACGA
TGAATGTCAGCAGCAGACCGTTCCCGCGGCGTCTGATGCCTCGCGTAGCGCCGCCGTTCGCGTAGTGGCGATGGTGGATG
CGCTGGCTGAGGTGGTGATTGCAAACGTTGATGAGTTAGGGCGCATTGACGCGGTAGCCGGTGATGGCGACCACGGTATG
GGTATGGAGCGCGGCGTGCGAGCAGCGCGCGAAAGTGCGCATCAATACCTGGCGCTGGGCGCAGGCGCAGGCACCGTTTT
GCAACAGGCCGCCGACGCGTGGGCTGATAACGCTGGCGGCACTTCCGGCGCGATTTGGGGCGTTATCTTAAATACCCTCG
GCACCGCTCTGGGTAATCAGCAGCAACCGCAGGCGGATACCGTCGTCCAGGCCGTGAGTGACGCAGCCCACGGGGTAATG
CATTTCGGTAAGGCTAAACCGGGTGACAAAACGCTGGTGGATGTCCTGCTGCCGTTTAGCGAAGCGCTGAATGAGAACGT
CAAAAAAGGTTTACCGCTGGCTCAGGCGTGGCACGCTGCCGCAAGTTATGCGCAGCAATGTGCGAAAGACACCTCACAGT
TGCTGCCGAAAATTGGTCGCGCCCGCCCGCTGGCTGAACGCAGCCTGGGAACGCCGGATGCGGGCGCCGTCTCGCTGGCG
ATGATTGTTACGGCAGTGGGCGGCTGTTTTACCGAAGCGGCTGGTCGTGAGAAAGAGGGGGCGGTATGGCAGTGA

Upstream 100 bases:

>100_bases
TTTATGCTGCTGTCGGCAATTCTGATGCTGGTATTAAGCCGTATTCCCCATACGTCCAGTGCCGCCGATGTGAAGATGAA
ACGAGCAGGAGAGCACTAAT

Downstream 100 bases:

>100_bases
ACCTCACTCTCGGCGTGAGCCTGAAAATGTATTTCGGTTATGCGCAAACCCTTGACTGGAGTCGGCAGGTAGCGGAAATC
GTGCGGCAACATCCGCTGTT

Product: dihydroxyacetone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MTWLFNNPSDFAKEMVAGFVSANADIVRQVPGGVIRNTQSQQGTVAIIIGGGSGHYPAFAGLVGQGLAHGAAMGNLFASP
SAQQICSVAKAANNGAGVLLAFGNYAGDVLHFGLARERLIAEGIPCELIAVTDDITSAGQHEPEKRRGVAGDLVVFKAAA
AAAEQGASLEEVLTVARHANDRTRTIGVAFSGCTLPGAEQPLFTIPQGQMGFGMGIHGEPGIREIAVPGADELATMLVEH
LLNDVPSGVSIKGARVGAILNGLGSVKYEELFVVWHSVQQRLNEQGIILADIQVGEFVTSFDMAGLSLTLIWFSEALEAQ
WQAPAYTSAFRRGAVIAAQPRQASSDDECQQQTVPAASDASRSAAVRVVAMVDALAEVVIANVDELGRIDAVAGDGDHGM
GMERGVRAARESAHQYLALGAGAGTVLQQAADAWADNAGGTSGAIWGVILNTLGTALGNQQQPQADTVVQAVSDAAHGVM
HFGKAKPGDKTLVDVLLPFSEALNENVKKGLPLAQAWHAAASYAQQCAKDTSQLLPKIGRARPLAERSLGTPDAGAVSLA
MIVTAVGGCFTEAAGREKEGAVWQ

Sequences:

>Translated_584_residues
MTWLFNNPSDFAKEMVAGFVSANADIVRQVPGGVIRNTQSQQGTVAIIIGGGSGHYPAFAGLVGQGLAHGAAMGNLFASP
SAQQICSVAKAANNGAGVLLAFGNYAGDVLHFGLARERLIAEGIPCELIAVTDDITSAGQHEPEKRRGVAGDLVVFKAAA
AAAEQGASLEEVLTVARHANDRTRTIGVAFSGCTLPGAEQPLFTIPQGQMGFGMGIHGEPGIREIAVPGADELATMLVEH
LLNDVPSGVSIKGARVGAILNGLGSVKYEELFVVWHSVQQRLNEQGIILADIQVGEFVTSFDMAGLSLTLIWFSEALEAQ
WQAPAYTSAFRRGAVIAAQPRQASSDDECQQQTVPAASDASRSAAVRVVAMVDALAEVVIANVDELGRIDAVAGDGDHGM
GMERGVRAARESAHQYLALGAGAGTVLQQAADAWADNAGGTSGAIWGVILNTLGTALGNQQQPQADTVVQAVSDAAHGVM
HFGKAKPGDKTLVDVLLPFSEALNENVKKGLPLAQAWHAAASYAQQCAKDTSQLLPKIGRARPLAERSLGTPDAGAVSLA
MIVTAVGGCFTEAAGREKEGAVWQ
>Mature_583_residues
TWLFNNPSDFAKEMVAGFVSANADIVRQVPGGVIRNTQSQQGTVAIIIGGGSGHYPAFAGLVGQGLAHGAAMGNLFASPS
AQQICSVAKAANNGAGVLLAFGNYAGDVLHFGLARERLIAEGIPCELIAVTDDITSAGQHEPEKRRGVAGDLVVFKAAAA
AAEQGASLEEVLTVARHANDRTRTIGVAFSGCTLPGAEQPLFTIPQGQMGFGMGIHGEPGIREIAVPGADELATMLVEHL
LNDVPSGVSIKGARVGAILNGLGSVKYEELFVVWHSVQQRLNEQGIILADIQVGEFVTSFDMAGLSLTLIWFSEALEAQW
QAPAYTSAFRRGAVIAAQPRQASSDDECQQQTVPAASDASRSAAVRVVAMVDALAEVVIANVDELGRIDAVAGDGDHGMG
MERGVRAARESAHQYLALGAGAGTVLQQAADAWADNAGGTSGAIWGVILNTLGTALGNQQQPQADTVVQAVSDAAHGVMH
FGKAKPGDKTLVDVLLPFSEALNENVKKGLPLAQAWHAAASYAQQCAKDTSQLLPKIGRARPLAERSLGTPDAGAVSLAM
IVTAVGGCFTEAAGREKEGAVWQ

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=572, Percent_Identity=31.1188811188811, Blast_Score=239, Evalue=5e-63,
Organism=Escherichia coli, GI87081857, Length=355, Percent_Identity=33.2394366197183, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1787449, Length=198, Percent_Identity=29.7979797979798, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17565018, Length=593, Percent_Identity=28.8364249578415, Blast_Score=205, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6321055, Length=561, Percent_Identity=30.6595365418895, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6323570, Length=591, Percent_Identity=29.2724196277496, Blast_Score=187, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 60444; Mature: 60313

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLFNNPSDFAKEMVAGFVSANADIVRQVPGGVIRNTQSQQGTVAIIIGGGSGHYPAFA
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCCCCCCEEEEEEECCCCCCHHHH
GLVGQGLAHGAAMGNLFASPSAQQICSVAKAANNGAGVLLAFGNYAGDVLHFGLARERLI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHH
AEGIPCELIAVTDDITSAGQHEPEKRRGVAGDLVVFKAAAAAAEQGASLEEVLTVARHAN
HCCCCEEEEEEECCHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
DRTRTIGVAFSGCTLPGAEQPLFTIPQGQMGFGMGIHGEPGIREIAVPGADELATMLVEH
CCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
LLNDVPSGVSIKGARVGAILNGLGSVKYEELFVVWHSVQQRLNEQGIILADIQVGEFVTS
HHHHCCCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHH
FDMAGLSLTLIWFSEALEAQWQAPAYTSAFRRGAVIAAQPRQASSDDECQQQTVPAASDA
HHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCC
SRSAAVRVVAMVDALAEVVIANVDELGRIDAVAGDGDHGMGMERGVRAARESAHQYLALG
CHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEC
AGAGTVLQQAADAWADNAGGTSGAIWGVILNTLGTALGNQQQPQADTVVQAVSDAAHGVM
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
HFGKAKPGDKTLVDVLLPFSEALNENVKKGLPLAQAWHAAASYAQQCAKDTSQLLPKIGR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ARPLAERSLGTPDAGAVSLAMIVTAVGGCFTEAAGREKEGAVWQ
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TWLFNNPSDFAKEMVAGFVSANADIVRQVPGGVIRNTQSQQGTVAIIIGGGSGHYPAFA
CCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCCCCCCEEEEEEECCCCCCHHHH
GLVGQGLAHGAAMGNLFASPSAQQICSVAKAANNGAGVLLAFGNYAGDVLHFGLARERLI
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHH
AEGIPCELIAVTDDITSAGQHEPEKRRGVAGDLVVFKAAAAAAEQGASLEEVLTVARHAN
HCCCCEEEEEEECCHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
DRTRTIGVAFSGCTLPGAEQPLFTIPQGQMGFGMGIHGEPGIREIAVPGADELATMLVEH
CCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHH
LLNDVPSGVSIKGARVGAILNGLGSVKYEELFVVWHSVQQRLNEQGIILADIQVGEFVTS
HHHHCCCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHH
FDMAGLSLTLIWFSEALEAQWQAPAYTSAFRRGAVIAAQPRQASSDDECQQQTVPAASDA
HHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCC
SRSAAVRVVAMVDALAEVVIANVDELGRIDAVAGDGDHGMGMERGVRAARESAHQYLALG
CHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEC
AGAGTVLQQAADAWADNAGGTSGAIWGVILNTLGTALGNQQQPQADTVVQAVSDAAHGVM
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
HFGKAKPGDKTLVDVLLPFSEALNENVKKGLPLAQAWHAAASYAQQCAKDTSQLLPKIGR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ARPLAERSLGTPDAGAVSLAMIVTAVGGCFTEAAGREKEGAVWQ
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]