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The map label for this gene is rbsB [H]

Identifier: 218547757

GI number: 218547757

Start: 354642

End: 355592

Strand: Direct

Name: rbsB [H]

Synonym: EFER_0342

Alternate gene names: 218547757

Gene position: 354642-355592 (Clockwise)

Preceding gene: 218547756

Following gene: 218547758

Centisome position: 7.73

GC content: 48.9

Gene sequence:

>951_bases
ATGTATAACAAAAGTAGACGTTTTATTCTGGCTGCAACCACCGTGGTGATGACCGCCTTCGCCACGCCAGTCTGGGCGGA
AGGGCTGATGACCATTATCGTTAACGATCCTTCTAACCCTTACTGGCTAACGGAAGGGGACGTTGCGCAAAAAACGGCAG
AAAAAATGGGTTATACCGCCAAGGTTAGTGCGCATAAAGGCGATACCAACACCGAAAGCCAACTGATCGACACGGCAATT
ACCAATAAATCCAAAGCGATTATTCTCGATCCGGCTAACGCAGATGGATCAATCGGATCGGTGAAAAAAGCGATCGCCGC
CAATATTCCGGTGTTTGTGATTAATGCTGAACTGAATCAAAGCGGGCTGGCGAAGGCACAGTTGGTTTCTAACAACGCAC
AAGGCGCGGCGTTAGCGGCCCAGCAATGGGTGCAACTGGTGGGCGATAACGGTGATTATGTAGAACTGAAAGGGCCACCT
TCAGACAATAACTCCGCAACCCGCGCTAACGGTTTTGAAACGGTACTTAGCCAGTATCCTGATTTGAAGAAATCCGGGTC
GGAGGTAGCAAACTGGGACCGCACTCAGGGATACAACAAGATGCAATCACTGTTACAGGCAAATCCCAATGTGAAAGGGG
TGATTAGCGGTAACGATGAAATGGCATTAGGGGCAATAGCGGCACTGAAAGAAGCCGGAAAATTGAAAGGGGTAACGGTC
GGCGGCTTTGATGGTTCGCCAGATGCCGTTGATGCGGTAAAAGACGGCACACTGGCTTATACCGTTATGCAGCCAGTTGC
CACGTTTGCGGCAAAAGCCATTGAACAGGCTGACACTTTCCTCAAAACCGGCAAAACAGGCGCGTCATCCGAGAAACAGT
TATTTGACTGTATTTTAATCACTAAAGAGAACGCAGCTAAAATTACGGCACCGTTTATGTTGTCAGAATAG

Upstream 100 bases:

>100_bases
GTCGGGGGCGTTAGTGAGTTTTTATCCAGCACGGAACCCGGTTGTATCCGTTCTGAAATCGTTCCAAAACTTTACCCTAA
AATAACGGAGCAACAACGCT

Downstream 100 bases:

>100_bases
TGCCGTGACCCGCGGGGGCGTTTTCGCCCCCCTTTATGATATTCTTATTACTCGCTGCCAATCCCGGCAATACAAACCCG
GAAGTGTTATGACGCAAATG

Product: ABC transporter periplasmic sugar-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MYNKSRRFILAATTVVMTAFATPVWAEGLMTIIVNDPSNPYWLTEGDVAQKTAEKMGYTAKVSAHKGDTNTESQLIDTAI
TNKSKAIILDPANADGSIGSVKKAIAANIPVFVINAELNQSGLAKAQLVSNNAQGAALAAQQWVQLVGDNGDYVELKGPP
SDNNSATRANGFETVLSQYPDLKKSGSEVANWDRTQGYNKMQSLLQANPNVKGVISGNDEMALGAIAALKEAGKLKGVTV
GGFDGSPDAVDAVKDGTLAYTVMQPVATFAAKAIEQADTFLKTGKTGASSEKQLFDCILITKENAAKITAPFMLSE

Sequences:

>Translated_316_residues
MYNKSRRFILAATTVVMTAFATPVWAEGLMTIIVNDPSNPYWLTEGDVAQKTAEKMGYTAKVSAHKGDTNTESQLIDTAI
TNKSKAIILDPANADGSIGSVKKAIAANIPVFVINAELNQSGLAKAQLVSNNAQGAALAAQQWVQLVGDNGDYVELKGPP
SDNNSATRANGFETVLSQYPDLKKSGSEVANWDRTQGYNKMQSLLQANPNVKGVISGNDEMALGAIAALKEAGKLKGVTV
GGFDGSPDAVDAVKDGTLAYTVMQPVATFAAKAIEQADTFLKTGKTGASSEKQLFDCILITKENAAKITAPFMLSE
>Mature_316_residues
MYNKSRRFILAATTVVMTAFATPVWAEGLMTIIVNDPSNPYWLTEGDVAQKTAEKMGYTAKVSAHKGDTNTESQLIDTAI
TNKSKAIILDPANADGSIGSVKKAIAANIPVFVINAELNQSGLAKAQLVSNNAQGAALAAQQWVQLVGDNGDYVELKGPP
SDNNSATRANGFETVLSQYPDLKKSGSEVANWDRTQGYNKMQSLLQANPNVKGVISGNDEMALGAIAALKEAGKLKGVTV
GGFDGSPDAVDAVKDGTLAYTVMQPVATFAAKAIEQADTFLKTGKTGASSEKQLFDCILITKENAAKITAPFMLSE

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=296, Percent_Identity=33.1081081081081, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1790526, Length=301, Percent_Identity=26.2458471760797, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790674, Length=220, Percent_Identity=29.5454545454545, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789990, Length=287, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788473, Length=233, Percent_Identity=30.9012875536481, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33311; Mature: 33311

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNKSRRFILAATTVVMTAFATPVWAEGLMTIIVNDPSNPYWLTEGDVAQKTAEKMGYTA
CCCCCCEEEEEHHHHHHHHHCCHHHHCCEEEEEEECCCCCEEEECCHHHHHHHHHCCCEE
KVSAHKGDTNTESQLIDTAITNKSKAIILDPANADGSIGSVKKAIAANIPVFVINAELNQ
EEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCC
SGLAKAQLVSNNAQGAALAAQQWVQLVGDNGDYVELKGPPSDNNSATRANGFETVLSQYP
CCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCC
DLKKSGSEVANWDRTQGYNKMQSLLQANPNVKGVISGNDEMALGAIAALKEAGKLKGVTV
CHHHCCCHHHCCHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEE
GGFDGSPDAVDAVKDGTLAYTVMQPVATFAAKAIEQADTFLKTGKTGASSEKQLFDCILI
ECCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEE
TKENAAKITAPFMLSE
ECCCCCEEECCEEECC
>Mature Secondary Structure
MYNKSRRFILAATTVVMTAFATPVWAEGLMTIIVNDPSNPYWLTEGDVAQKTAEKMGYTA
CCCCCCEEEEEHHHHHHHHHCCHHHHCCEEEEEEECCCCCEEEECCHHHHHHHHHCCCEE
KVSAHKGDTNTESQLIDTAITNKSKAIILDPANADGSIGSVKKAIAANIPVFVINAELNQ
EEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCC
SGLAKAQLVSNNAQGAALAAQQWVQLVGDNGDYVELKGPPSDNNSATRANGFETVLSQYP
CCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCC
DLKKSGSEVANWDRTQGYNKMQSLLQANPNVKGVISGNDEMALGAIAALKEAGKLKGVTV
CHHHCCCHHHCCHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEE
GGFDGSPDAVDAVKDGTLAYTVMQPVATFAAKAIEQADTFLKTGKTGASSEKQLFDCILI
ECCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEE
TKENAAKITAPFMLSE
ECCCCCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]