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The map label for this gene is yadI [H]

Identifier: 218547585

GI number: 218547585

Start: 166238

End: 166678

Strand: Direct

Name: yadI [H]

Synonym: EFER_0150

Alternate gene names: 218547585

Gene position: 166238-166678 (Clockwise)

Preceding gene: 218547584

Following gene: 218547586

Centisome position: 3.62

GC content: 50.79

Gene sequence:

>441_bases
ATGTTAGGTTGGGTAATTACCTGTCACGATGATCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACACGGGGCGCT
TCTTCAGTGCCGGGCCGTGAATTTCTGGCGAGGATTAAGTTCTAATATGCTCAGCCGTATGATGTGCGATGCTCTGCATG
AAGCAGACTCTGGCGATGGGGTGATCTTTTTAACTGATGTTGCCGGAGCGCCGCCGTACCGCGTGGCTTCGCTATTAAGC
CACAAACACTCCCGCTGCGAAGTGATTTCTGGGGTAACGTTACCGTTAGTTGAACAGATGCTGGCCTACCGTGAAACGTT
ATCCAGTGCGGAGTTTCGCGAGCGAATTGTCGAACTGGGTGGGCCTGAAGTTAGTAGTCTCTGGCACCAACAACAAAAGA
ATCCCCCCTTTGTCCTCAAGCATAATTGGTTCGAGTATTAA

Upstream 100 bases:

>100_bases
ATTTCCTCTCCTCTGGATTCGGGGGAGAGGAGTTTTGAAGGCTATCACCCTTTATCAACAATGGTCAGGGTAGACTGATT
TTCGGCTAAGGAGGAAGGTG

Downstream 100 bases:

>100_bases
ATCGCATTCCGATTTTGTTACACTAACGGCGCTGTTTCTCGCCTGGTAGTTTTCTCATGATTAAAAAGATTATTTTTTTA
CTGCTGCTGGTTTCATCTGG

Product: PTS Enzyme IIA

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGDGVIFLTDVAGAPPYRVASLLS
HKHSRCEVISGVTLPLVEQMLAYRETLSSAEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNWFEY

Sequences:

>Translated_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGDGVIFLTDVAGAPPYRVASLLS
HKHSRCEVISGVTLPLVEQMLAYRETLSSAEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNWFEY
>Mature_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGDGVIFLTDVAGAPPYRVASLLS
HKHSRCEVISGVTLPLVEQMLAYRETLSSAEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNWFEY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1786321, Length=146, Percent_Identity=92.4657534246575, Blast_Score=290, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16535; Mature: 16535

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGDG
CCCEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
VIFLTDVAGAPPYRVASLLSHKHSRCEVISGVTLPLVEQMLAYRETLSSAEFRERIVELG
EEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPEVSSLWHQQQKNPPFVLKHNWFEY
CCHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGDG
CCCEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
VIFLTDVAGAPPYRVASLLSHKHSRCEVISGVTLPLVEQMLAYRETLSSAEFRERIVELG
EEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPEVSSLWHQQQKNPPFVLKHNWFEY
CCHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]