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The map label for this gene is leuO [H]

Identifier: 218547534

GI number: 218547534

Start: 106327

End: 107289

Strand: Direct

Name: leuO [H]

Synonym: EFER_0097

Alternate gene names: 218547534

Gene position: 106327-107289 (Clockwise)

Preceding gene: 218547524

Following gene: 218547535

Centisome position: 2.32

GC content: 45.69

Gene sequence:

>963_bases
GTGACAGTGGAGTTTAATATGCCAGAGGTAAAATTTGATCAGCCAAGTCTTTCTGAGATAGGCAGACCACAGCTTCGCAT
GGTCGATCTGAATCTGTTAACCGTTTTTGATGCGGTGATGCAAGAGCAAAATATCACACGTGCCGCACAAACTCTGGGGA
TGTCACAGCCTGCGGTGAGTAACGCCGTGGCGCGCCTGAAAGTGATGTTTAATGATGAGTTGTTTGTCCGGTATGGTCGG
GGGATCCAACCGACTGCTCGTGCGTATCAGTTGTTTGGTTCGGTAAGACAGGCTTTGCAATTAGTGCAGAATGAATTGCC
CGGGTCTGGTTTCGAACCAGCCAGTAGCGAACGGATATTTAATCTTTGTGTTTGCAGTCCGCTGGACAATCTGCTGACCT
CATTGGTTTATAATCGCATCAATGATATTGCCCCAAATATTCACGTTGTTTTTAAATCTTCTCTTAATCAAAACACTGAA
CACCAATTGCGTTATCAGGAAATCGAATTTGTCATTGGTTATGAAGAATTCCGCCGTCCTGAGTTTTCCAGCGTACCGTT
ATTTAATGATGAAATGGTGCTGGTTGCCAGCAAGAAACATCCGACGATTAATGGCCCGCTGTTGGAAAACGATGTTTATC
AATTGCAGCACGCGGTTGTATCTCTGGAGCGTTTTGCTTCGTTCAGTCAACCCTGGTATGACACGACTGAAAAACAGGGC
TGTATTGCTTATCAGGGAATGGCATTGATCAGTGTGTTGAATGTGGTTTCGCAAACCCATCTGGTTTCCATTGCGCCGCG
ATGGTTAGCCGAGGAGTTTGCTGAATCACTGGAATTACAAGTGCTTCCACTGCCTTTAAAACTGAATAGTAGAACATGTT
ATCTTTCGTGGCATGAAGCTGCCGGACGTGACAAAGGCCATCAATGGATGGAAGAGTTATTGGTCTCCGTCTGCCGCCAT
TAA

Upstream 100 bases:

>100_bases
CCTAATTTTCGTGTTTGCCCTTTTTCTTATTTTATATGCATGGTAAATCATATTTTCCGAGATTATTTCTCAGCATTCCG
GCAAGAAAAGGGAGCTAAGC

Downstream 100 bases:

>100_bases
TCTTATCGCAATAAACCAGATCGAATGGTCTGGTTTATTCTGCTTTTTTTAAAAAATATGAAATTTTATGCTGTATTACT
GATGGGGCTTTCTCAACAGA

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTVEFNMPEVKFDQPSLSEIGRPQLRMVDLNLLTVFDAVMQEQNITRAAQTLGMSQPAVSNAVARLKVMFNDELFVRYGR
GIQPTARAYQLFGSVRQALQLVQNELPGSGFEPASSERIFNLCVCSPLDNLLTSLVYNRINDIAPNIHVVFKSSLNQNTE
HQLRYQEIEFVIGYEEFRRPEFSSVPLFNDEMVLVASKKHPTINGPLLENDVYQLQHAVVSLERFASFSQPWYDTTEKQG
CIAYQGMALISVLNVVSQTHLVSIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKGHQWMEELLVSVCRH

Sequences:

>Translated_320_residues
MTVEFNMPEVKFDQPSLSEIGRPQLRMVDLNLLTVFDAVMQEQNITRAAQTLGMSQPAVSNAVARLKVMFNDELFVRYGR
GIQPTARAYQLFGSVRQALQLVQNELPGSGFEPASSERIFNLCVCSPLDNLLTSLVYNRINDIAPNIHVVFKSSLNQNTE
HQLRYQEIEFVIGYEEFRRPEFSSVPLFNDEMVLVASKKHPTINGPLLENDVYQLQHAVVSLERFASFSQPWYDTTEKQG
CIAYQGMALISVLNVVSQTHLVSIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKGHQWMEELLVSVCRH
>Mature_319_residues
TVEFNMPEVKFDQPSLSEIGRPQLRMVDLNLLTVFDAVMQEQNITRAAQTLGMSQPAVSNAVARLKVMFNDELFVRYGRG
IQPTARAYQLFGSVRQALQLVQNELPGSGFEPASSERIFNLCVCSPLDNLLTSLVYNRINDIAPNIHVVFKSSLNQNTEH
QLRYQEIEFVIGYEEFRRPEFSSVPLFNDEMVLVASKKHPTINGPLLENDVYQLQHAVVSLERFASFSQPWYDTTEKQGC
IAYQGMALISVLNVVSQTHLVSIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKGHQWMEELLVSVCRH

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=313, Percent_Identity=84.6645367412141, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI87081766, Length=287, Percent_Identity=25.7839721254355, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786820, Length=286, Percent_Identity=25.1748251748252, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36390; Mature: 36259

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEFNMPEVKFDQPSLSEIGRPQLRMVDLNLLTVFDAVMQEQNITRAAQTLGMSQPAVS
CEEECCCCCCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
NAVARLKVMFNDELFVRYGRGIQPTARAYQLFGSVRQALQLVQNELPGSGFEPASSERIF
HHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
NLCVCSPLDNLLTSLVYNRINDIAPNIHVVFKSSLNQNTEHQLRYQEIEFVIGYEEFRRP
HHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEEECHHHHCCC
EFSSVPLFNDEMVLVASKKHPTINGPLLENDVYQLQHAVVSLERFASFSQPWYDTTEKQG
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
CIAYQGMALISVLNVVSQTHLVSIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEA
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEHHH
AGRDKGHQWMEELLVSVCRH
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVEFNMPEVKFDQPSLSEIGRPQLRMVDLNLLTVFDAVMQEQNITRAAQTLGMSQPAVS
EEECCCCCCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
NAVARLKVMFNDELFVRYGRGIQPTARAYQLFGSVRQALQLVQNELPGSGFEPASSERIF
HHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
NLCVCSPLDNLLTSLVYNRINDIAPNIHVVFKSSLNQNTEHQLRYQEIEFVIGYEEFRRP
HHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEEECHHHHCCC
EFSSVPLFNDEMVLVASKKHPTINGPLLENDVYQLQHAVVSLERFASFSQPWYDTTEKQG
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
CIAYQGMALISVLNVVSQTHLVSIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEA
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEHHH
AGRDKGHQWMEELLVSVCRH
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]