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The map label for this gene is fadH [C]

Identifier: 218547528

GI number: 218547528

Start: 98552

End: 100564

Strand: Reverse

Name: fadH [C]

Synonym: EFER_0092

Alternate gene names: 218547528

Gene position: 100564-98552 (Counterclockwise)

Preceding gene: 218547529

Following gene: 218547527

Centisome position: 2.19

GC content: 50.57

Gene sequence:

>2013_bases
ATGAGCCACTACCCCCATCTGTTCAGCCCGCTGACCATCAACGGAATGACCATCAAAAACCGGATAATCATGCCGCCAAT
GGGCACCAATCTGGCGAGTCTGAACGGTGAAGTATCGCAAGAGCAGCTTGATTACTATGAACTTCGTGCACGTGGCGGCA
CGGGCCTGATTACCATTGAGAATATCTGCATCGATTTCCCTTTTGCCTCCAACGGCACCACGCAATTACGTATCGATAAC
GATCAATATATTCCTGGTCTGTTTAAACTTACCGAAACCCTTCACCGCCACGGCGCGTGTGTCGCCATACAATTAAATCA
CGCTGGTGCTTCCGCTTATGCCTATCGTCTGAATGGTGAAACGCCGCTCTCCTCCTCCAGTACGCCATCGAAGAAAAACG
GCAACACGCCGCGCCCGATGACTCATGAGGAGATCTATCACGCAATAGATAAATTTGGTGAAGCCGCTGAACGTGTGCGT
CGCGCTAATTTTGATTGTGTGGAGATCCACGGTGGTCACTCCTATTTAATCAGCCAGTTTTTATCGCCGCTCTTTAATAA
ACGTGACGATGAATTTGGTGGCAGCCTGGAAAACCGAGCCCGCTTTTTAACCCTGGTGGTTGATAAAGTACGCGCCAGCG
TAGGCCCACGCTTCCCTGTCAGCTTACGTATCAGTGCCGATGATTTCCTCGCAGGTGGCAACACGCTGGAGGACTCACTG
CGTATTCTTGAACTCTGTCAGGATAAAGTGGATATCATCAACGTATCGGCAGCGCAAAACGACAGCCTGAATCTGCAAAT
CGACCAGATGTCACTGGAAGATGGCTGGAAGCGCTACCTTTCTCGTGCCGTGCGTGACAAATTCCATAAACCTACCGTTA
TCGCGGGTAATATTCGTTCTCCCAAAGTGGCTGAAGATATTGTGGCCAGCGGTGATGCAGACCTTATCGCCATTGGTCGT
GGGCTGATTGCTGAACCGGAGTGGGTACAAAAAGTACAAAGTGGCAATGAACGGCTGATGCGTAAATGCATCTCCTGCAA
TATCGGTTGTGCCGATCACCGTATTGCCCGCTCGCGCCCTCTGCGTTGTTCCATTAACCCGGATATTATCCATGGCGACG
CCTACAAGCTGCGTAACGTTACTCGTACTACCAACGTGGTTGTTATTGGCGGCGGTACGGCGGGAATGGAAGCCGCCTGT
ACAGCAGCTGAAGTCGGTTGCATGACCTGGCTTCTGGAAGAGAAAACACACCTCGGTGGTCTTTCCTGCGAAATATCCCG
TCTGCCGGAAAAAACGCGTATTGCTGACTTCCCTCTGTTTATGAAAAACCGCCTCGCGTCACTCAACAACCTGATGTTAC
AGGTCGGTAAGCGTGCGGATGTCACCAGTGTGGCAGCATTGCATCCTGACTTAATTGTTAACGCCACCGGTTCGGTTCCT
CTGTTACCGCCTATCGACAGTTTACAGGAACACATTGATCTCGCAGACGGTAACGTATTTTCCATTACCGGCATGATCAA
CAATCTCGACCGATTTACGAAGACGCAAGGCAAGCGTATCGCCGTTGTTGGCGGCGGTGCTGTAGGTCTGGACGTGGTCG
AATATTTTGCTACCCGTGGCGCGAACGTAACACTTATTGAGATGCTGGACGCCCCGGCACGTGACCTCGATATCATCACC
AAAAACGCCATGTTAAATCTGCTGGAAGAACATCGCGTAGAGCAGCATATGCAAACACAGCTAATGGCTGTTTCCGCTGA
TCATTTCACCGTTAAAAACGCACAAAGCACCTTCGATATCCCCTTTGATTACGGCTTTATCTGTTTAGGGATGCGAGCCA
ATCGGGCCGGATTTGACGACATCGACCGCTGGGCCAGAGAAAACAACATCAAAATGATGAATATAGGCGATAGTGTAATG
GCGCGCCGGATTATTGATGGTGTGCGGGAAGGCCGCAGCGTACTGGATACCCTGGAAGATATGGGTGCCCTGGGAAAAGA
ATTTCATTTCTAA

Upstream 100 bases:

>100_bases
TTTTCTTGCAACCGCCTCGTTATCTTGCCCGCAGTGAAATATTAACTTTGGACCCACTGCAGGCGTATGTCTGTCCCTGG
GCATCCAGTGGAGAGATGAC

Downstream 100 bases:

>100_bases
CAACTTGAGGACGTTATCATGGCAGAACGTATTACCGGGCATACTGAACTGATTGGGCTTATTGCGACACCTATTCGTCA
TAGCATGTCTCCAACAATGC

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MSHYPHLFSPLTINGMTIKNRIIMPPMGTNLASLNGEVSQEQLDYYELRARGGTGLITIENICIDFPFASNGTTQLRIDN
DQYIPGLFKLTETLHRHGACVAIQLNHAGASAYAYRLNGETPLSSSSTPSKKNGNTPRPMTHEEIYHAIDKFGEAAERVR
RANFDCVEIHGGHSYLISQFLSPLFNKRDDEFGGSLENRARFLTLVVDKVRASVGPRFPVSLRISADDFLAGGNTLEDSL
RILELCQDKVDIINVSAAQNDSLNLQIDQMSLEDGWKRYLSRAVRDKFHKPTVIAGNIRSPKVAEDIVASGDADLIAIGR
GLIAEPEWVQKVQSGNERLMRKCISCNIGCADHRIARSRPLRCSINPDIIHGDAYKLRNVTRTTNVVVIGGGTAGMEAAC
TAAEVGCMTWLLEEKTHLGGLSCEISRLPEKTRIADFPLFMKNRLASLNNLMLQVGKRADVTSVAALHPDLIVNATGSVP
LLPPIDSLQEHIDLADGNVFSITGMINNLDRFTKTQGKRIAVVGGGAVGLDVVEYFATRGANVTLIEMLDAPARDLDIIT
KNAMLNLLEEHRVEQHMQTQLMAVSADHFTVKNAQSTFDIPFDYGFICLGMRANRAGFDDIDRWARENNIKMMNIGDSVM
ARRIIDGVREGRSVLDTLEDMGALGKEFHF

Sequences:

>Translated_670_residues
MSHYPHLFSPLTINGMTIKNRIIMPPMGTNLASLNGEVSQEQLDYYELRARGGTGLITIENICIDFPFASNGTTQLRIDN
DQYIPGLFKLTETLHRHGACVAIQLNHAGASAYAYRLNGETPLSSSSTPSKKNGNTPRPMTHEEIYHAIDKFGEAAERVR
RANFDCVEIHGGHSYLISQFLSPLFNKRDDEFGGSLENRARFLTLVVDKVRASVGPRFPVSLRISADDFLAGGNTLEDSL
RILELCQDKVDIINVSAAQNDSLNLQIDQMSLEDGWKRYLSRAVRDKFHKPTVIAGNIRSPKVAEDIVASGDADLIAIGR
GLIAEPEWVQKVQSGNERLMRKCISCNIGCADHRIARSRPLRCSINPDIIHGDAYKLRNVTRTTNVVVIGGGTAGMEAAC
TAAEVGCMTWLLEEKTHLGGLSCEISRLPEKTRIADFPLFMKNRLASLNNLMLQVGKRADVTSVAALHPDLIVNATGSVP
LLPPIDSLQEHIDLADGNVFSITGMINNLDRFTKTQGKRIAVVGGGAVGLDVVEYFATRGANVTLIEMLDAPARDLDIIT
KNAMLNLLEEHRVEQHMQTQLMAVSADHFTVKNAQSTFDIPFDYGFICLGMRANRAGFDDIDRWARENNIKMMNIGDSVM
ARRIIDGVREGRSVLDTLEDMGALGKEFHF
>Mature_669_residues
SHYPHLFSPLTINGMTIKNRIIMPPMGTNLASLNGEVSQEQLDYYELRARGGTGLITIENICIDFPFASNGTTQLRIDND
QYIPGLFKLTETLHRHGACVAIQLNHAGASAYAYRLNGETPLSSSSTPSKKNGNTPRPMTHEEIYHAIDKFGEAAERVRR
ANFDCVEIHGGHSYLISQFLSPLFNKRDDEFGGSLENRARFLTLVVDKVRASVGPRFPVSLRISADDFLAGGNTLEDSLR
ILELCQDKVDIINVSAAQNDSLNLQIDQMSLEDGWKRYLSRAVRDKFHKPTVIAGNIRSPKVAEDIVASGDADLIAIGRG
LIAEPEWVQKVQSGNERLMRKCISCNIGCADHRIARSRPLRCSINPDIIHGDAYKLRNVTRTTNVVVIGGGTAGMEAACT
AAEVGCMTWLLEEKTHLGGLSCEISRLPEKTRIADFPLFMKNRLASLNNLMLQVGKRADVTSVAALHPDLIVNATGSVPL
LPPIDSLQEHIDLADGNVFSITGMINNLDRFTKTQGKRIAVVGGGAVGLDVVEYFATRGANVTLIEMLDAPARDLDIITK
NAMLNLLEEHRVEQHMQTQLMAVSADHFTVKNAQSTFDIPFDYGFICLGMRANRAGFDDIDRWARENNIKMMNIGDSVMA
RRIIDGVREGRSVLDTLEDMGALGKEFHF

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=542, Percent_Identity=28.2287822878229, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1787939, Length=368, Percent_Identity=30.4347826086957, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=308, Percent_Identity=29.2207792207792, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559802, Length=350, Percent_Identity=26.5714285714286, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=308, Percent_Identity=26.6233766233766, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540738, Length=343, Percent_Identity=23.9067055393586, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=316, Percent_Identity=27.8481012658228, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17566914, Length=350, Percent_Identity=26.5714285714286, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=309, Percent_Identity=26.537216828479, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321973, Length=235, Percent_Identity=30.6382978723404, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6325086, Length=364, Percent_Identity=25.8241758241758, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 73887; Mature: 73756

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYPHLFSPLTINGMTIKNRIIMPPMGTNLASLNGEVSQEQLDYYELRARGGTGLITIE
CCCCCCCCCCEEECCEEEECEEEECCCCCCHHHCCCCCCHHHHHHHHEEECCCCEEEEEE
NICIDFPFASNGTTQLRIDNDQYIPGLFKLTETLHRHGACVAIQLNHAGASAYAYRLNGE
EEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCC
TPLSSSSTPSKKNGNTPRPMTHEEIYHAIDKFGEAAERVRRANFDCVEIHGGHSYLISQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
LSPLFNKRDDEFGGSLENRARFLTLVVDKVRASVGPRFPVSLRISADDFLAGGNTLEDSL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCCHHHHH
RILELCQDKVDIINVSAAQNDSLNLQIDQMSLEDGWKRYLSRAVRDKFHKPTVIAGNIRS
HHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCCCC
PKVAEDIVASGDADLIAIGRGLIAEPEWVQKVQSGNERLMRKCISCNIGCADHRIARSRP
CHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHCCC
LRCSINPDIIHGDAYKLRNVTRTTNVVVIGGGTAGMEAACTAAEVGCMTWLLEEKTHLGG
CEEECCCCEEECCHHHHCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LSCEISRLPEKTRIADFPLFMKNRLASLNNLMLQVGKRADVTSVAALHPDLIVNATGSVP
CEEEHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCC
LLPPIDSLQEHIDLADGNVFSITGMINNLDRFTKTQGKRIAVVGGGAVGLDVVEYFATRG
CCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCC
ANVTLIEMLDAPARDLDIITKNAMLNLLEEHRVEQHMQTQLMAVSADHFTVKNAQSTFDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCEECC
PFDYGFICLGMRANRAGFDDIDRWARENNIKMMNIGDSVMARRIIDGVREGRSVLDTLED
CCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
MGALGKEFHF
HHHHHHHCCC
>Mature Secondary Structure 
SHYPHLFSPLTINGMTIKNRIIMPPMGTNLASLNGEVSQEQLDYYELRARGGTGLITIE
CCCCCCCCCEEECCEEEECEEEECCCCCCHHHCCCCCCHHHHHHHHEEECCCCEEEEEE
NICIDFPFASNGTTQLRIDNDQYIPGLFKLTETLHRHGACVAIQLNHAGASAYAYRLNGE
EEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCC
TPLSSSSTPSKKNGNTPRPMTHEEIYHAIDKFGEAAERVRRANFDCVEIHGGHSYLISQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
LSPLFNKRDDEFGGSLENRARFLTLVVDKVRASVGPRFPVSLRISADDFLAGGNTLEDSL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCCHHHHH
RILELCQDKVDIINVSAAQNDSLNLQIDQMSLEDGWKRYLSRAVRDKFHKPTVIAGNIRS
HHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCCCC
PKVAEDIVASGDADLIAIGRGLIAEPEWVQKVQSGNERLMRKCISCNIGCADHRIARSRP
CHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHCCC
LRCSINPDIIHGDAYKLRNVTRTTNVVVIGGGTAGMEAACTAAEVGCMTWLLEEKTHLGG
CEEECCCCEEECCHHHHCCCEEECEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LSCEISRLPEKTRIADFPLFMKNRLASLNNLMLQVGKRADVTSVAALHPDLIVNATGSVP
CEEEHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCC
LLPPIDSLQEHIDLADGNVFSITGMINNLDRFTKTQGKRIAVVGGGAVGLDVVEYFATRG
CCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCC
ANVTLIEMLDAPARDLDIITKNAMLNLLEEHRVEQHMQTQLMAVSADHFTVKNAQSTFDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCEECC
PFDYGFICLGMRANRAGFDDIDRWARENNIKMMNIGDSVMARRIIDGVREGRSVLDTLED
CCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
MGALGKEFHF
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]