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The map label for this gene is kefF [H]

Identifier: 218547493

GI number: 218547493

Start: 56418

End: 56948

Strand: Direct

Name: kefF [H]

Synonym: EFER_0054

Alternate gene names: 218547493

Gene position: 56418-56948 (Clockwise)

Preceding gene: 218547492

Following gene: 218547494

Centisome position: 1.23

GC content: 54.24

Gene sequence:

>531_bases
ATGATCCTCATAATTTATGCGCATCCGTATCCGCATCATTCTCATGCGAATAAACGGATGCTTGAACACGCAAGGACGCT
GGAAGGCGTCGAAGTTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCTGCCGAGCAGGAGGCGTTGT
CTCGCGCCGACCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCACCGCTCCTCAAACTTTGGATCGATAAA
GTTTTCTCGCACGGCTGGGCTTACGGTCACGGCGGCACGGCACTGCATGGTAAACATTTGCTGTGGGCGGTGACGACCGG
CGGCGGGGAAAGTCATTTTGAGATTGGCGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCT
ACTGCGGGCTGAACTGGCTGCCACCGTTTGCTATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCG
CGTCATTATAAGCAACGTCTGCTGGAATGGCAGGAGGCATATCATGGATAG

Upstream 100 bases:

>100_bases
AGATTTCTGCCTTTTTTGAATCAATATTATTGACTATTAGCTGCGTGAATATGTGACTACACTTTGTGGGAAAACAAAGG
CGTAAACACGCGAGTTAACT

Downstream 100 bases:

>100_bases
CCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCGGCGCTGATTGTACCTATTGCGGTGCGTCTTGGGCTTGGCT
CGGTTCTTGGCTACCTGATC

Product: glutathione-regulated potassium-efflux system ancillary protein KefF

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MILIIYAHPYPHHSHANKRMLEHARTLEGVEVRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDK
VFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
RHYKQRLLEWQEAYHG

Sequences:

>Translated_176_residues
MILIIYAHPYPHHSHANKRMLEHARTLEGVEVRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDK
VFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
RHYKQRLLEWQEAYHG
>Mature_176_residues
MILIIYAHPYPHHSHANKRMLEHARTLEGVEVRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDK
VFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
RHYKQRLLEWQEAYHG

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=151, Percent_Identity=33.7748344370861, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4505415, Length=104, Percent_Identity=35.5769230769231, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1786231, Length=176, Percent_Identity=98.2954545454545, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789750, Length=165, Percent_Identity=41.2121212121212, Blast_Score=115, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 20191; Mature: 20191

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIIYAHPYPHHSHANKRMLEHARTLEGVEVRSLYQLYPDFNIDIAAEQEALSRADLIV
CEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECHHHHHHHHCEEE
WQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPG
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEEECCCCCCEEEECCCCC
FDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAYHG
HHHHHCCHHHHEEEECCCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILIIYAHPYPHHSHANKRMLEHARTLEGVEVRSLYQLYPDFNIDIAAEQEALSRADLIV
CEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECHHHHHHHHCEEE
WQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPG
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEEEECCCCCCEEEECCCCC
FDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAYHG
HHHHHCCHHHHEEEECCCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA