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The map label for this gene is leuD

Identifier: 218236062

GI number: 218236062

Start: 1398748

End: 1399329

Strand: Direct

Name: leuD

Synonym: BCB4264_A1456

Alternate gene names: 218236062

Gene position: 1398748-1399329 (Clockwise)

Preceding gene: 218234850

Following gene: 218233691

Centisome position: 25.81

GC content: 34.71

Gene sequence:

>582_bases
ATGGAGCCATTTCGTATTCATAAAGGTACTGCCGCAGTACTGATGAATGATAACATTGATACAGATCAAATTATTCCGAA
GCAATATTTAAAGAGAATTGAAAGAACTGGGTTTGGGAAGTTTTTATTTGATGAGTGGCGATATGATAATAATCGCCAAG
AAAACCCTAATTTCCCGCTTAATGCACAAGAAAGAAAAGGAGCCAGCATATTAATCACAGGTGATAATTTTGGATGTGGC
TCGTCAAGAGAACATGCTCCATGGGCACTTGCTGATTATGGTTTCCGCGTTATTATCGCTGGAGGATTTGCCGATATCTT
TTATATGAATTGTATGAAGAATGGCATGTTACCGATTGTAATGGATAAAGATATGCGTGAACAACTTGCTAAAACAGATG
CGAGGGAACAAATTACAGTTGATTTAGAAAATGAAATAATGACAACGAATACGCATAGATTCCATTTTACGATCGAGAAA
ATGTGGAAAGAGAAGTTATTAAATGGTTTAGATGAAATTAGTATTACAATGCAATATGAACAAGAAATAAAAGAGTATGA
AAGAAAAGTGGCTTTACATTAA

Upstream 100 bases:

>100_bases
AGGCAAGGAAAGGGAGCACGAACGCATTTAGTTAGCCCGGCAATGGCGGCAGCCGCTGCGTTATATGGTCATTTTGTTGA
TATTAGAAAGGAGAGTTATG

Downstream 100 bases:

>100_bases
AAAAATGACGGAATATTAAAAAAGTATTTACATTCAAAATTAACTGTTATATACTATGTGAAAATCAGAGGAGAAAGGGA
GTAAGTATTATGAATATCGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNNRQENPNFPLNAQERKGASILITGDNFGCG
SSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKDMREQLAKTDAREQITVDLENEIMTTNTHRFHFTIEK
MWKEKLLNGLDEISITMQYEQEIKEYERKVALH

Sequences:

>Translated_193_residues
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNNRQENPNFPLNAQERKGASILITGDNFGCG
SSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKDMREQLAKTDAREQITVDLENEIMTTNTHRFHFTIEK
MWKEKLLNGLDEISITMQYEQEIKEYERKVALH
>Mature_193_residues
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNNRQENPNFPLNAQERKGASILITGDNFGCG
SSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKDMREQLAKTDAREQITVDLENEIMTTNTHRFHFTIEK
MWKEKLLNGLDEISITMQYEQEIKEYERKVALH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=193, Percent_Identity=46.6321243523316, Blast_Score=180, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=46.9387755102041, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_BACCR (Q81G09)

Other databases:

- EMBL:   AE016877
- RefSeq:   NP_831183.1
- ProteinModelPortal:   Q81G09
- SMR:   Q81G09
- STRING:   Q81G09
- EnsemblBacteria:   EBBACT00000033743
- GeneID:   1203752
- GenomeReviews:   AE016877_GR
- KEGG:   bce:BC1403
- eggNOG:   COG0066
- GeneTree:   EBGT00050000002923
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   BCER226900:BC_1403-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22590; Mature: 22590

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNNRQENPNFPL
CCCCEECCCEEEEEEECCCCCHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
NAQERKGASILITGDNFGCGSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCEEEE
MDKDMREQLAKTDAREQITVDLENEIMTTNTHRFHFTIEKMWKEKLLNGLDEISITMQYE
ECHHHHHHHHHCCCCCEEEEEECCCEEECCCEEEEEEHHHHHHHHHHCCHHHEEEEEHHH
QEIKEYERKVALH
HHHHHHHHHHCCC
>Mature Secondary Structure
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNNRQENPNFPL
CCCCEECCCEEEEEEECCCCCHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
NAQERKGASILITGDNFGCGSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCEEEE
MDKDMREQLAKTDAREQITVDLENEIMTTNTHRFHFTIEKMWKEKLLNGLDEISITMQYE
ECHHHHHHHHHCCCCCEEEEEECCCEEECCCEEEEEEHHHHHHHHHHCCHHHEEEEEHHH
QEIKEYERKVALH
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630