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The map label for this gene is hisZ [H]

Identifier: 218233691

GI number: 218233691

Start: 1399639

End: 1400901

Strand: Direct

Name: hisZ [H]

Synonym: BCB4264_A1457

Alternate gene names: 218233691

Gene position: 1399639-1400901 (Clockwise)

Preceding gene: 218236062

Following gene: 218234195

Centisome position: 25.83

GC content: 34.44

Gene sequence:

>1263_bases
ATGACAAAATGGAAACGAGCAAACCCGAATGGAACGAGAGATTATTTATTCGAAGAATGTACGTTAATTGAAGAAGTAGA
ACAAAAATTAAGGCTTACTTTTTTAGAGAGAGGTTATGAGGAAATAAGAACACCTACAATTGAATTTTATGATGTTTTTG
CATTTCAAAATAGACCGATAGATGAAGAGAAGATGTATAAATTTTTTGATGAAAAGGGAAGGATTATCGTTTTACGTCCA
GATATGACAATTCCTTTAGCGAGAGTTATTGGAACACAGAGATGGGATACGCCACTTAAAGTGACGTATAGCGGGAATGT
ATTTCGAGCGAATGAATCCCATTCTGGAAAATATAATGAAATCGTACAAAGTGGTATTGAAGTTATCGGGATTGATAATG
TAAGAGCTGAAATTGAATGTGTAATAAGTGTCATTCAAGCACTTCAAAAGTTGAAGGTGCAATCTTTTACAATTGAGATT
GGGCAAGTACAGTTATATAAATGTATTGTAAAAAAACTCTCCATCCAGGATGAAGAAGAAAGGGTACTTAGAACGTATAT
AGAAAGTAAAAATTATGCAGCCCTCTCAAATTTTATCGGAGAAAAGAAATTAGATCGATGCGATGAGACAGTAAGATTAC
TTGAGAAATTGCCAAGGCTATTTGGAAATTTAGAAGTTATTGAGGAAGCAGAAAAGCTTGCTTCAAGTAATGAAATGAAA
ATGGCAATTGCAAGAGTAAAAGAAATGTATGAAACAATTGAAATGTTAGGATATGGTTCTTACATATCAATTGATTTGGG
CATGATTCAACACTTAGATTACTATACGGGTGTTATTTTCAAAGGATATATATATGAAATTGGAGAAGAAATCGTTAGTG
GCGGAAGGTACGATGAGCTAATTGGGAATTTTGGAGAAATGCTGCCAGCTGTTGGACTCGCGGTACAAGTAAATCAAATT
GTGAAGGCGCTGCAAGAGCAGCAAGAACCATATGAACGAAATCAAATTGATATTGTGATTCATTATGAGTTAAATAGATT
AGCGGAAGCAGAAAGGCTACGAAATTTACTTCGAAAGGATGGGAAAAATGCTTGGTTATCTCTATTCTCTAATTTAAGCG
ATACCTTTCAATTTGCAAGAAAAAATAAAATAGGAACGGTCGTTGAGGCAAAGAATGAATACTTAGTGGAATATGTATGG
AATGAGAAATGGGTAGTCCAGAAAGAAGGAGAGGCTTCATGCGTAACATTCAAATTGCGTTAA

Upstream 100 bases:

>100_bases
GAAGAGCCTTGTAGGTATTTTGTCTACAAGGCTCTTTTTATTTTCGGCTTAGGGGAATGAAATAGGCATACATTAGAAGG
GGAAAGGTGTGAAAATTTAG

Downstream 100 bases:

>100_bases
CGAAAGGAAGATTAGAAAAACATGTCATTCCACTTTTTGAGCAGATTGGAATTGATTGTTCCGAGCTCAAAAATAAAGGT
CGGAAGCTTGTATTTCAAAG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRLTFLERGYEEIRTPTIEFYDVFAFQNRPIDEEKMYKFFDEKGRIIVLRP
DMTIPLARVIGTQRWDTPLKVTYSGNVFRANESHSGKYNEIVQSGIEVIGIDNVRAEIECVISVIQALQKLKVQSFTIEI
GQVQLYKCIVKKLSIQDEEERVLRTYIESKNYAALSNFIGEKKLDRCDETVRLLEKLPRLFGNLEVIEEAEKLASSNEMK
MAIARVKEMYETIEMLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQI
VKALQEQQEPYERNQIDIVIHYELNRLAEAERLRNLLRKDGKNAWLSLFSNLSDTFQFARKNKIGTVVEAKNEYLVEYVW
NEKWVVQKEGEASCVTFKLR

Sequences:

>Translated_420_residues
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRLTFLERGYEEIRTPTIEFYDVFAFQNRPIDEEKMYKFFDEKGRIIVLRP
DMTIPLARVIGTQRWDTPLKVTYSGNVFRANESHSGKYNEIVQSGIEVIGIDNVRAEIECVISVIQALQKLKVQSFTIEI
GQVQLYKCIVKKLSIQDEEERVLRTYIESKNYAALSNFIGEKKLDRCDETVRLLEKLPRLFGNLEVIEEAEKLASSNEMK
MAIARVKEMYETIEMLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQI
VKALQEQQEPYERNQIDIVIHYELNRLAEAERLRNLLRKDGKNAWLSLFSNLSDTFQFARKNKIGTVVEAKNEYLVEYVW
NEKWVVQKEGEASCVTFKLR
>Mature_419_residues
TKWKRANPNGTRDYLFEECTLIEEVEQKLRLTFLERGYEEIRTPTIEFYDVFAFQNRPIDEEKMYKFFDEKGRIIVLRPD
MTIPLARVIGTQRWDTPLKVTYSGNVFRANESHSGKYNEIVQSGIEVIGIDNVRAEIECVISVIQALQKLKVQSFTIEIG
QVQLYKCIVKKLSIQDEEERVLRTYIESKNYAALSNFIGEKKLDRCDETVRLLEKLPRLFGNLEVIEEAEKLASSNEMKM
AIARVKEMYETIEMLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQIV
KALQEQQEPYERNQIDIVIHYELNRLAEAERLRNLLRKDGKNAWLSLFSNLSDTFQFARKNKIGTVVEAKNEYLVEYVWN
EKWVVQKEGEASCVTFKLR

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=353, Percent_Identity=24.929178470255, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI15029520, Length=348, Percent_Identity=25.2873563218391, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=438, Percent_Identity=23.0593607305936, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993693, Length=438, Percent_Identity=23.0593607305936, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=361, Percent_Identity=22.9916897506925, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24643061, Length=359, Percent_Identity=26.4623955431755, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24643059, Length=377, Percent_Identity=25.4641909814324, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 48860; Mature: 48729

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRLTFLERGYEEIRTPTIEFYDVFAFQNRPI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEECCCCC
DEEKMYKFFDEKGRIIVLRPDMTIPLARVIGTQRWDTPLKVTYSGNVFRANESHSGKYNE
CHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCHHHH
IVQSGIEVIGIDNVRAEIECVISVIQALQKLKVQSFTIEIGQVQLYKCIVKKLSIQDEEE
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHCCCCHHH
RVLRTYIESKNYAALSNFIGEKKLDRCDETVRLLEKLPRLFGNLEVIEEAEKLASSNEMK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHH
MAIARVKEMYETIEMLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDEL
HHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IGNFGEMLPAVGLAVQVNQIVKALQEQQEPYERNQIDIVIHYELNRLAEAERLRNLLRKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHC
GKNAWLSLFSNLSDTFQFARKNKIGTVVEAKNEYLVEYVWNEKWVVQKEGEASCVTFKLR
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
TKWKRANPNGTRDYLFEECTLIEEVEQKLRLTFLERGYEEIRTPTIEFYDVFAFQNRPI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEECCCCC
DEEKMYKFFDEKGRIIVLRPDMTIPLARVIGTQRWDTPLKVTYSGNVFRANESHSGKYNE
CHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCHHHH
IVQSGIEVIGIDNVRAEIECVISVIQALQKLKVQSFTIEIGQVQLYKCIVKKLSIQDEEE
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHCCCCHHH
RVLRTYIESKNYAALSNFIGEKKLDRCDETVRLLEKLPRLFGNLEVIEEAEKLASSNEMK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHH
MAIARVKEMYETIEMLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDEL
HHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IGNFGEMLPAVGLAVQVNQIVKALQEQQEPYERNQIDIVIHYELNRLAEAERLRNLLRKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHC
GKNAWLSLFSNLSDTFQFARKNKIGTVVEAKNEYLVEYVWNEKWVVQKEGEASCVTFKLR
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA