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The map label for this gene is cls [H]

Identifier: 218235896

GI number: 218235896

Start: 1212569

End: 1214113

Strand: Direct

Name: cls [H]

Synonym: BCB4264_A1245

Alternate gene names: 218235896

Gene position: 1212569-1214113 (Clockwise)

Preceding gene: 218235246

Following gene: 218234492

Centisome position: 22.38

GC content: 33.66

Gene sequence:

>1545_bases
ATGAAAAATACGTTAAAGCTGATTTTCTTTATACTGCTATTGTTCGCATTATTTGTTTCGTTACGTATGTTTATTGATGT
AGCATTTTATTCAGATGTGATTGGGATAAAAGATGTTTCGATTTTAGGTATTATTAGTATTTTATTTACTGTATCTGCGT
TTTTAATTGGTTGCGTTATTTTCTTAGAAAATCGACATCCATCTAAAACACTTACATGGTTAATTGTTTTAGGAATTTTT
CCGGTATTTGGTTTCTTTGCTTATTTATTATTTGGACAAAATTTTCGAAGAAAAAGAATGTTCCAAAAGAAGGCGTTACT
TGATGAACAGGCGTTTTTACAATATAAAGGGCATGAAGATTACGAAGAACGAATTTTACGCAATCATAAGCATCAAGAAC
TGTTATTTCGTTTAGCGGATCGATTAGGAGCTTTAAATATTTCATTTCAAACTGAAACGAGGACATTAACAAATGGAGAT
GAAACGTTTCGGGCTATTTTAAATGGGTTAAAACGAGCGAAACATCACATTCATATGGAATATTACATTGTGCGTGATGA
TAAATTAGGAACAGAAATTAAAGATATTTTAATACAAAAATCGAAAGAAGGCGTTGTTGTTCGCTTTTTATATGATGCGG
TTGGAAGTTTTAAGTTATCGAAATCGTATATTGAAGAATTAAACGATGCAGGTGTAGAAATGATTCCATTCTTTCCTGTG
CGCTTTCCGATTTTAAACGATAAGATTAATTATCGAAATCATAGGAAAATTGTTGTGATTGATGGGAACGAAGGGTTTGT
AGGCGGATTAAATATCGGTGATGAGTATTTAGGGAAAAATAAATATTTTGGCTTTTGGCGAGATACGCATTTGTATTTAC
GTGGTGAAGCGGTTCAAAGTTTACAACTTATTTTCCTTCAAGACTGGTTTTATATGACTGGAGAGGCTGTGCTAGCCCCA
GAATATTTACAAGCAAAAGCAGTTGAGGGTGATCATTGGGGAGGAGTGCAGCTAGTTGCAGGTGGCCCCGATAATAAGTG
GGAAACGATTAAGCATTTATATTTCGCAATGATCGCTTCTGCTCGGAAATCAATTTGGATTGCAACGCCGTATTTTATTC
CGGATGATGATATTTTATCCGCATTAAAGGTTGCTGCACTTGCTGGTATTGATGTTCGTTTATTAATGCCGAGTAAACCG
GATAAGCGTACTGTCTTTTACGCATCGAGATCGTACTTCCCAGAGCTTTTAGATGCAGGAGTAAAGATATATGAATACGA
AAAAGGTTTTCTTCATAGTAAAATTGTCATCGTTGATTCTGATTTAGCTTCAATTGGGACAGCTAATATGGATATGAGAA
GTTTTCATTTGAATTTTGAAGTAAATGCCTTTTTATATGATACAGATAGCATTCGAAAACTTGTTCAAGACTTTAAAGAT
GATTTAGAAGAATCAAGTGAAATTCATGGTGATCATTTTCATAAAAGACGTCTTCATAGACGAATTGTTGAATCAACGTA
CCGATTATTATCACCTTTATTATAA

Upstream 100 bases:

>100_bases
TCGCAATAATTTTCCTGCTAAAACGTAGGCCGATTTCTGTAGTATGAAATCGGCTTTTTATATGAAGAATAGGAAAGCTT
TCTTAGTTAGGAGGTAAGAG

Downstream 100 bases:

>100_bases
AAAGAGATGTCCGAAATTGGGACATCTCTTTTTTAAAAGGAATTTGTAACAAATTGTAGAATATAAGGAGCTGAAAGGGT
GTGATTACGATATTTATCGC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKNTLKLIFFILLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFRAILNGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGKNKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKIVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHGDHFHKRRLHRRIVESTYRLLSPLL

Sequences:

>Translated_514_residues
MKNTLKLIFFILLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFRAILNGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGKNKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKIVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHGDHFHKRRLHRRIVESTYRLLSPLL
>Mature_514_residues
MKNTLKLIFFILLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFRAILNGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGKNKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKIVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHGDHFHKRRLHRRIVESTYRLLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=464, Percent_Identity=29.5258620689655, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1787007, Length=334, Percent_Identity=29.6407185628743, Blast_Score=138, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59467; Mature: 59467

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTLKLIFFILLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
YEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGDETFRAILNGLKRAKHHIHME
HHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
EEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEECCEE
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGKNKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCCEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAKAVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKIVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLEESSEIHGDHFHKRRLHRRIVESTYRLLSPLL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNTLKLIFFILLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
YEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGDETFRAILNGLKRAKHHIHME
HHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
EEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEECCEE
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGKNKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCCEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAKAVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKIVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLEESSEIHGDHFHKRRLHRRIVESTYRLLSPLL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]