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The map label for this gene is thiN [H]

Identifier: 218235867

GI number: 218235867

Start: 3879551

End: 3880192

Strand: Reverse

Name: thiN [H]

Synonym: BCB4264_A3958

Alternate gene names: 218235867

Gene position: 3880192-3879551 (Counterclockwise)

Preceding gene: 218230927

Following gene: 218232987

Centisome position: 71.6

GC content: 36.92

Gene sequence:

>642_bases
ATGATTATTCATATTTTGGCAGGAGGACCTTCGGAATATTGCGCAGATTTTGCTCGATACGAGAATGAGGAAGTAGTGTG
GGCAGCGGTTGATAGGGGAGTATACCGTCTATTAAAGAAAGGGATTACCCCGGCTGTTGCGTTTGGAGATTACGATTCAG
TTACTGAAGAAGAATTAGTATGGATGGGACAGCAAACGAACGAATTACATATTGTTCCGCGGGAAAAAGATCAAACGGAT
TTAGAAATTGCAATCAACTGGGCATTAGAACAGAAGCCGGCACTAATTCGTATTTTTGGTGCTACTGGTGGAAGGCTTGA
TCACGGTTTGGCAAATATACAGATGCTTTTAAAAGGGTTAGAAGTAGAGATAGAAATGTGTATTGTGGACAATAAAAATG
AAATAACAGTTAAAAAAGTGGGTACACATATAATTGAAGAAAATAAAAAATTTCCTTATGTATCTTTTGTACCAGTTACT
GAAATAGTGGAAGGGATTACATTGTTTGGTTTTAAGTACCCGCTTACTAATAAAACAATAGAGTGGGGATCAACACTTTG
TATTAGTAACGAACTCGTTGAGGAAAAAGGTACTTTTTCATTTACTTCTGGCATATTAATGTTGATAAGAAGCACTGATT
GA

Upstream 100 bases:

>100_bases
TTTTATCCTAGTAAGGCATTTATAGGATAGGATAAAAATATGTAAAAAGGCAATCTACCGAATGGCAGATTGCCTTTTTA
CAATGGAAGGAGAAGAAAAA

Downstream 100 bases:

>100_bases
AGAAACAATTTTGCTCCTTACATCATATAGTGACCAAGTGGAGTAATGGGGATTAGGAGGGAAACCATGAGATTTTATAC
AATTAAGTTACCTAAATTTC

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MIIHILAGGPSEYCADFARYENEEVVWAAVDRGVYRLLKKGITPAVAFGDYDSVTEEELVWMGQQTNELHIVPREKDQTD
LEIAINWALEQKPALIRIFGATGGRLDHGLANIQMLLKGLEVEIEMCIVDNKNEITVKKVGTHIIEENKKFPYVSFVPVT
EIVEGITLFGFKYPLTNKTIEWGSTLCISNELVEEKGTFSFTSGILMLIRSTD

Sequences:

>Translated_213_residues
MIIHILAGGPSEYCADFARYENEEVVWAAVDRGVYRLLKKGITPAVAFGDYDSVTEEELVWMGQQTNELHIVPREKDQTD
LEIAINWALEQKPALIRIFGATGGRLDHGLANIQMLLKGLEVEIEMCIVDNKNEITVKKVGTHIIEENKKFPYVSFVPVT
EIVEGITLFGFKYPLTNKTIEWGSTLCISNELVEEKGTFSFTSGILMLIRSTD
>Mature_213_residues
MIIHILAGGPSEYCADFARYENEEVVWAAVDRGVYRLLKKGITPAVAFGDYDSVTEEELVWMGQQTNELHIVPREKDQTD
LEIAINWALEQKPALIRIFGATGGRLDHGLANIQMLLKGLEVEIEMCIVDNKNEITVKKVGTHIIEENKKFPYVSFVPVT
EIVEGITLFGFKYPLTNKTIEWGSTLCISNELVEEKGTFSFTSGILMLIRSTD

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71997787, Length=215, Percent_Identity=21.8604651162791, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23941; Mature: 23941

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIHILAGGPSEYCADFARYENEEVVWAAVDRGVYRLLKKGITPAVAFGDYDSVTEEELV
CEEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEECCCCCCCHHHHE
WMGQQTNELHIVPREKDQTDLEIAINWALEQKPALIRIFGATGGRLDHGLANIQMLLKGL
EECCCCCEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
EVEIEMCIVDNKNEITVKKVGTHIIEENKKFPYVSFVPVTEIVEGITLFGFKYPLTNKTI
CEEEEEEEECCCCCEEEEHHHHHHHHCCCCCCEEEECCHHHHHCCCEEEEEECCCCCCEE
EWGSTLCISNELVEEKGTFSFTSGILMLIRSTD
CCCCEEEECHHHHHCCCCEEECCEEEEEEEECC
>Mature Secondary Structure
MIIHILAGGPSEYCADFARYENEEVVWAAVDRGVYRLLKKGITPAVAFGDYDSVTEEELV
CEEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEECCCCCCCHHHHE
WMGQQTNELHIVPREKDQTDLEIAINWALEQKPALIRIFGATGGRLDHGLANIQMLLKGL
EECCCCCEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
EVEIEMCIVDNKNEITVKKVGTHIIEENKKFPYVSFVPVTEIVEGITLFGFKYPLTNKTI
CEEEEEEEECCCCCEEEEHHHHHHHHCCCCCCEEEECCHHHHHCCCEEEEEECCCCCCEE
EWGSTLCISNELVEEKGTFSFTSGILMLIRSTD
CCCCEEEECHHHHHCCCCEEECCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]