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The map label for this gene is glcF [H]

Identifier: 218235653

GI number: 218235653

Start: 1306177

End: 1307505

Strand: Reverse

Name: glcF [H]

Synonym: BCB4264_A1347

Alternate gene names: 218235653

Gene position: 1307505-1306177 (Counterclockwise)

Preceding gene: 218235034

Following gene: 218233578

Centisome position: 24.13

GC content: 36.79

Gene sequence:

>1329_bases
ATGACCACATTAAATAAAGAAAATATTCAAAAAGAATTTAAAGAACGATTAAGTGAAGATGAATTACTAAACTGTATGCG
ATGCGGTTTTTGTTTACCTACTTGTCCTACCTACATTCAATCCGGATATAAAGAGTCACATTCACCGCGCGGACGTATTG
CATTAATGAAAGCAGTCGTTGATGGTTTAATCGAACCAGATGAAGATGTTGAAAATACATTAAACGTTTGTCTTGGTTGC
CGTGCTTGTGAGCCTGTTTGTCCATCTGGTGTAAATTATGGACATTTATTAGAAGAAACTCGTGATATTATTAATCAAAA
TAAAAAATTCTCAGTGCCAGTGAAAGCTGTTCGAAAAGTCGTTTTTGAAGGATTATTCCCCCATCAAAATCGAATGAGAA
CATTAACTGGACTAATCGGATTTTATCAACGTTCTGGTTTACAAACGCTAACACATAAAACAGGGATTATGAAGTTATTC
CCTGAAACCCTTGCAACGATGGATCTCGTTTTACCAAAAGTCCCTAAAATGAAAGCGATGAAAGATCGTCCTTCATTTTT
ACCTGCAGAAAGTACAAAGAAAAAACAAGTTGCATTCTTTACAGGCTGCTTAATGGATACGATGTTTTTAGAAACAAATA
ATGCAACGATGAAACTTCTTCAATTAGCTGGTTGTGATATCGTTATTCCGAAAACACAAAGTTGCTGCGGTGCATTGCAT
GGACATGCTGGTGAAAAGAGTGGAGCAAAAGAATTAGCAAAGCGAAATATAAAAGCATTCGAAAATTTAAACATCGACTA
TATTATTACGAATGCAGGTGGTTGCGGGGCTTATCTTGTAGACTATGATTATCTTTTAAAGGATGATCCGGATTGGGCTG
AACGTGCTAAGCAGTTTGTTTCTAAAATTAAAGATATTACTGCTATACTAGTAGAACTAGACTTCCATAAGCGAACTGAC
TTACGACTCACGCCGCAGGTTATTACGTATCAAGACTCTTGTCATTTACGCAATGTTATGCGCACATCTTCTGAACCTCG
TATGTTACTAGAGGCTATCCAAGGTGCAACATATCGAGAAATGAAAGATGCCGACCGCTGCTGTGGCTCTGCTGGTATTT
ACAATATTGTTCATTCAGAGTTATCAATGGAATTTCTAGATTACAAAATGGACCGTGTGCATGAAACAGAAGCAGCAACC
ATTGTTACTGCAAATCCAGGTTGTTTATTACAAATGAAATTGGGCATTGAGCGAGAAGGGCTTTCTCATAAAATGAGAGG
AATTCACATTGTGGATTTATTATTAGAAGCAATTGAAACTAACTCGTAA

Upstream 100 bases:

>100_bases
CAGCTATGCAAGGAATAAAGCAAGCTTTCGATCCAAATAACATTATGAATCCTGGAAAAATGTTCGCGAAAGACACTCGA
AAAAGAGTGGTGGTTGAGCG

Downstream 100 bases:

>100_bases
TAACTCGTAACATACGTAATAATAGGAAAACGGCTATCTCCTTCGTCTATGTAAAGAGATAGCCGCTTTTTTGTCTGCTA
CAACATTAAAAATATGTTAC

Product: putative glycolate oxidase, iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEETRDIINQNKKFSVPVKAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPSFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKSGAKELAKRNIKAFENLNIDYIITNAGGCGAYLVDYDYLLKDDPDWAERAKQFVSKIKDITAILVELDFHKRTD
LRLTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAAT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Sequences:

>Translated_442_residues
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEETRDIINQNKKFSVPVKAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPSFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKSGAKELAKRNIKAFENLNIDYIITNAGGCGAYLVDYDYLLKDDPDWAERAKQFVSKIKDITAILVELDFHKRTD
LRLTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAAT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS
>Mature_441_residues
TTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGCR
ACEPVCPSGVNYGHLLEETRDIINQNKKFSVPVKAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFP
ETLATMDLVLPKVPKMKAMKDRPSFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALHG
HAGEKSGAKELAKRNIKAFENLNIDYIITNAGGCGAYLVDYDYLLKDDPDWAERAKQFVSKIKDITAILVELDFHKRTDL
RLTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAATI
VTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=451, Percent_Identity=28.8248337028825, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786497, Length=240, Percent_Identity=28.75, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49672; Mature: 49541

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEETRDIINQNKKFSVPVKAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPSFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKSGAKELAKRNIKAFENLNIDYIITNAGGCGAYLVDYDYLLKDDPDWAERAKQFV
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECHHHHCCCCCHHHHHHHHH
SKIKDITAILVELDFHKRTDLRLTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCCEEEECCHHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAATIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEETRDIINQNKKFSVPVKAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPSFLPAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKSGAKELAKRNIKAFENLNIDYIITNAGGCGAYLVDYDYLLKDDPDWAERAKQFV
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECHHHHCCCCCHHHHHHHHH
SKIKDITAILVELDFHKRTDLRLTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCCEEEECCHHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAATIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]