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The map label for this gene is glcD [H]

Identifier: 218235034

GI number: 218235034

Start: 1307502

End: 1308914

Strand: Reverse

Name: glcD [H]

Synonym: BCB4264_A1348

Alternate gene names: 218235034

Gene position: 1308914-1307502 (Counterclockwise)

Preceding gene: 218233562

Following gene: 218235653

Centisome position: 24.15

GC content: 38.71

Gene sequence:

>1413_bases
ATGTTAGAAAAACAAATTATTCATTCATTCGTATCTATTGTTGGCGAAGATAATGTAGATACATCCAATATGGGGCGTTT
AACTTATAGTTATGATGCCACTCCAAACTTCCAAGCAATGCCTGATGCAGTCATTGCTCCTCGTAATACAAATGAAGTAG
CTGAAGTGTTAAAAGTATGTAACACTCACAAAATCCCTGTGTATGTGCGCGGGTCTGGAACAAACCTTTGCGCAGGAACA
TGTCCACTTGAAGGCGGTATCGTCCTTATTTTCCGCCATATGAACAATATTTTAGAAATTGATGAAGAAAACTTAACAAT
TACTGTACAAGCTGGTGTCATTACACTTGATATTATTAAAGCGGTAGAAGAAAAAGGTTTATTTTATCCACCAGATCCAA
GCTCGATGAAAATTTCTACAATCGGCGGTAATATTAATGAAAACTCAGGTGGATTACGCGGTTTGAAATATGGCGTAACA
CGTGATTATGTAATGGGGCTTGAACTTGTCTTACCAAATGGCGATATTATTCGCACTGGTGGTAAATTAGCAAAAGATGT
AGCTGGTTACGATTTAACTCGTTTATTTATCGGTTCTGAAGGAACACTTGGCGTGGTAACAGAAGCTATATTAAAACTTG
TTCCTATGCCTGAAACAAAGAAAACAATGCTTGCACTATATGAAGATATTAATGAAGCTGCACGTGCTGTTTCTTCTATT
ATTGCAAATAAAATCATTCCAGCAACACTGGAGTTTTTAGACCAACCGACAATTGAAGTAGTAGAAGCGTTTGCACAAAT
CGGTTTACCTACAGATGTAAAAGCAATTTTATTAATCGAACAAGACGGTCCGCCCGAAGTAGTCAATCGAGATATTGAAA
AAATGGCTAACGTTTGTCACTCTATGAATGCGGTTGATGTTCGCGTTGCAAAAGGTGAAACAGAAGCAGATGCGCTTCGT
ACCGCTCGCCGTAGTGCTCTATCAGCGCTCGCACGATTAAAACCTACTACAATATTAGAAGATGCGACTGTACCACGTTC
ACAAATCGCTCCGATGGTTGAAGCTATTAATGCCATTGCAAAAAAATATAATATTCCTATTTGTACGTTTGGACATGCTG
GTGATGGTAATTTACATCCAACTTGTATGACAGACGCTCGTAATGCAGAAGAGATGCACCGAGCTGAGCAAGCTTTTGCT
GAAATATTTGCGAAAGCAATTGAACTTGGTGGCACAATTACTGGTGAACATGGTGTTGGTGCAATGAAAGCTCCATATTT
AGAAATGAAATTAGGTAAAGAAGGTATTGCAGCTATGCAAGGAATAAAGCAAGCTTTCGATCCAAATAACATTATGAATC
CTGGAAAAATGTTCGCGAAAGACACTCGAAAAAGAGTGGTGGTTGAGCGATGA

Upstream 100 bases:

>100_bases
ATAGAAAGAATCCCTCGACTTTTTTATCATTATAATGAAAGCACTGTTACCACGTTGTTTATTATAACTAAACACGTTAA
CAGTTTGAGGGGGATTTCCA

Downstream 100 bases:

>100_bases
CCACATTAAATAAAGAAAATATTCAAAAAGAATTTAAAGAACGATTAAGTGAAGATGAATTACTAAACTGTATGCGATGC
GGTTTTTGTTTACCTACTTG

Product: glycolate oxidase, subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLEKQIIHSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEVAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEAFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCHSMNAVDVRVAKGETEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHAGDGNLHPTCMTDARNAEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGIAAMQGIKQAFDPNNIMNPGKMFAKDTRKRVVVER

Sequences:

>Translated_470_residues
MLEKQIIHSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEVAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEAFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCHSMNAVDVRVAKGETEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHAGDGNLHPTCMTDARNAEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGIAAMQGIKQAFDPNNIMNPGKMFAKDTRKRVVVER
>Mature_470_residues
MLEKQIIHSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEVAEVLKVCNTHKIPVYVRGSGTNLCAGT
CPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIKAVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVMGLELVLPNGDIIRTGGKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
IANKIIPATLEFLDQPTIEVVEAFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCHSMNAVDVRVAKGETEADALR
TARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIAKKYNIPICTFGHAGDGNLHPTCMTDARNAEEMHRAEQAFA
EIFAKAIELGGTITGEHGVGAMKAPYLEMKLGKEGIAAMQGIKQAFDPNNIMNPGKMFAKDTRKRVVVER

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=467, Percent_Identity=31.0492505353319, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI37595754, Length=490, Percent_Identity=29.5918367346939, Blast_Score=233, Evalue=4e-61,
Organism=Homo sapiens, GI119964728, Length=432, Percent_Identity=28.0092592592593, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4501993, Length=232, Percent_Identity=28.8793103448276, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789351, Length=430, Percent_Identity=37.6744186046512, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1787977, Length=514, Percent_Identity=27.0428015564202, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI48994907, Length=486, Percent_Identity=26.1316872427983, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI48994914, Length=177, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=456, Percent_Identity=32.6754385964912, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI71992381, Length=456, Percent_Identity=32.2368421052632, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71992392, Length=384, Percent_Identity=32.2916666666667, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17534361, Length=465, Percent_Identity=28.8172043010753, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17556096, Length=453, Percent_Identity=23.3995584988962, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320027, Length=445, Percent_Identity=31.0112359550562, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6320764, Length=474, Percent_Identity=28.4810126582279, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320023, Length=453, Percent_Identity=28.6975717439294, Blast_Score=173, Evalue=6e-44,
Organism=Drosophila melanogaster, GI18921117, Length=474, Percent_Identity=27.8481012658228, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24639277, Length=474, Percent_Identity=27.8481012658228, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24639275, Length=474, Percent_Identity=27.8481012658228, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24653753, Length=255, Percent_Identity=27.843137254902, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50825; Mature: 50825

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKQIIHSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEVAEVLKVC
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
NTHKIPVYVRGSGTNLCAGTCPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHHHEEECCCCEEEEEECCEEHHHHHH
AVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVMGLELVLPNGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IANKIIPATLEFLDQPTIEVVEAFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCH
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SMNAVDVRVAKGETEADALRTARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
KKYNIPICTFGHAGDGNLHPTCMTDARNAEEMHRAEQAFAEIFAKAIELGGTITGEHGVG
HHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEMKLGKEGIAAMQGIKQAFDPNNIMNPGKMFAKDTRKRVVVER
CCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLEKQIIHSFVSIVGEDNVDTSNMGRLTYSYDATPNFQAMPDAVIAPRNTNEVAEVLKVC
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
NTHKIPVYVRGSGTNLCAGTCPLEGGIVLIFRHMNNILEIDEENLTITVQAGVITLDIIK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHHHEEECCCCEEEEEECCEEHHHHHH
AVEEKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVMGLELVLPNGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFIGSEGTLGVVTEAILKLVPMPETKKTMLALYEDINEAARAVSSI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IANKIIPATLEFLDQPTIEVVEAFAQIGLPTDVKAILLIEQDGPPEVVNRDIEKMANVCH
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
SMNAVDVRVAKGETEADALRTARRSALSALARLKPTTILEDATVPRSQIAPMVEAINAIA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
KKYNIPICTFGHAGDGNLHPTCMTDARNAEEMHRAEQAFAEIFAKAIELGGTITGEHGVG
HHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEMKLGKEGIAAMQGIKQAFDPNNIMNPGKMFAKDTRKRVVVER
CCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]