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The map label for this gene is prpE [H]

Identifier: 218235507

GI number: 218235507

Start: 1224370

End: 1225110

Strand: Reverse

Name: prpE [H]

Synonym: BCB4264_A1257

Alternate gene names: 218235507

Gene position: 1225110-1224370 (Counterclockwise)

Preceding gene: 218236079

Following gene: 218234984

Centisome position: 22.61

GC content: 39.27

Gene sequence:

>741_bases
ATGAAATATGACATTATAGGAGATATTCATGGATGCTTCCAAGAGTTTCAAAATTTAACAGAGAAGCTAGGATATAACTG
GAGTAGCGGTCTACCGGTTCACCCTGACCAACGTAAACTTGCATTCGTTGGCGATATTACAGACCGTGGTCCTCATTCAT
TACGTATGATTGAAATTGTATGGGAACTTGTCATACATAAAAAAGAAGCTTATTACGCTCCTGGAAATCACTGTAATAAA
CTATATCGATTTTTCCTTGGGCGTAATGTAACAGTCGCTCACGGACTTGAAACAACTGTTGCTGAATATGAGGCACTTCC
TTCTCATAAGCAAAACATTATAAAAGAAAAGTTTATCACTCTTTATGAACAATCACCGCTCTACCACATACTAGATGAAA
AAAGAGTAATCGTATGTCACGCTGGGATTCGGCAAGATTACATAGGAAGACGAGATAAAAAAGTACAAACCTTTGTATTA
TATGGCGATATTACAGGAGAAAAGCATGCCGATGGCTCACCTGTCCGTCGCGATTGGGCGCAAGAGTACAAGGGGCAAGC
TTGGATTGTATATGGACATACACCAGTAGCAGAACCTCGATTCATCAATCAGACAGTCAATATTGATACTGGTGCTGTAT
TTGGTGGCAAATTAACCGGATTACGTTATCCTGAAATGGAGACTATCTCTGTTCCTTCTTCTTTACCTTTTGTCGCTGAA
AAATTCCGTCCAATTTCATAA

Upstream 100 bases:

>100_bases
ACGAAAATCTTTATGTTTAAATGAACAACACAAAAACGCTATATTTTTATCTCTATCCTTTTTTAGATATACTAATGAAA
AATACGATGGAGGTTTAAAT

Downstream 100 bases:

>100_bases
CTACCTTACTTAGCGAAAGATTACCGTATTATTTAGGCAGATCTCATATAAAACTAGGTAAAGTAATTAAAAAAAGATGC
AATAAAGTGCATCTTTTTTT

Product: bis(5'-nucleosyl)-tetraphosphatase PrpE

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKYDIIGDIHGCFQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKEAYYAPGNHCNK
LYRFFLGRNVTVAHGLETTVAEYEALPSHKQNIIKEKFITLYEQSPLYHILDEKRVIVCHAGIRQDYIGRRDKKVQTFVL
YGDITGEKHADGSPVRRDWAQEYKGQAWIVYGHTPVAEPRFINQTVNIDTGAVFGGKLTGLRYPEMETISVPSSLPFVAE
KFRPIS

Sequences:

>Translated_246_residues
MKYDIIGDIHGCFQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKEAYYAPGNHCNK
LYRFFLGRNVTVAHGLETTVAEYEALPSHKQNIIKEKFITLYEQSPLYHILDEKRVIVCHAGIRQDYIGRRDKKVQTFVL
YGDITGEKHADGSPVRRDWAQEYKGQAWIVYGHTPVAEPRFINQTVNIDTGAVFGGKLTGLRYPEMETISVPSSLPFVAE
KFRPIS
>Mature_246_residues
MKYDIIGDIHGCFQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIVWELVIHKKEAYYAPGNHCNK
LYRFFLGRNVTVAHGLETTVAEYEALPSHKQNIIKEKFITLYEQSPLYHILDEKRVIVCHAGIRQDYIGRRDKKVQTFVL
YGDITGEKHADGSPVRRDWAQEYKGQAWIVYGHTPVAEPRFINQTVNIDTGAVFGGKLTGLRYPEMETISVPSSLPFVAE
KFRPIS

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.6.1.17 [H]

Molecular weight: Translated: 28207; Mature: 28207

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDIIGDIHGCFQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIV
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
WELVIHKKEAYYAPGNHCNKLYRFFLGRNVTVAHGLETTVAEYEALPSHKQNIIKEKFIT
HHHHHHHCCCEECCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
LYEQSPLYHILDEKRVIVCHAGIRQDYIGRRDKKVQTFVLYGDITGEKHADGSPVRRDWA
HHCCCCCEEECCCCEEEEEECCCCHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
QEYKGQAWIVYGHTPVAEPRFINQTVNIDTGAVFGGKLTGLRYPEMETISVPSSLPFVAE
HHHCCCEEEEECCCCCCCCCEECEEECCCCCEEECCEECCCCCCCCEEEECCCCCCHHHH
KFRPIS
HCCCCC
>Mature Secondary Structure
MKYDIIGDIHGCFQEFQNLTEKLGYNWSSGLPVHPDQRKLAFVGDITDRGPHSLRMIEIV
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
WELVIHKKEAYYAPGNHCNKLYRFFLGRNVTVAHGLETTVAEYEALPSHKQNIIKEKFIT
HHHHHHHCCCEECCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
LYEQSPLYHILDEKRVIVCHAGIRQDYIGRRDKKVQTFVLYGDITGEKHADGSPVRRDWA
HHCCCCCEEECCCCEEEEEECCCCHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
QEYKGQAWIVYGHTPVAEPRFINQTVNIDTGAVFGGKLTGLRYPEMETISVPSSLPFVAE
HHHCCCEEEEECCCCCCCCCEECEEECCCCCEEECCEECCCCCCCCEEEECCCCCCHHHH
KFRPIS
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA