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The map label for this gene is kipI [H]

Identifier: 218235467

GI number: 218235467

Start: 2960696

End: 2961409

Strand: Reverse

Name: kipI [H]

Synonym: BCB4264_A3088

Alternate gene names: 218235467

Gene position: 2961409-2960696 (Counterclockwise)

Preceding gene: 218234495

Following gene: 218235693

Centisome position: 54.65

GC content: 37.96

Gene sequence:

>714_bases
ATGAGATTTTCTGCGTTAGGGGATCAAGCAATTATTGTTACATTTGGTGAAGAAATTAAGAGGGATATATATGAGAAAGT
ACAGCGGTTATTTCAAGCACTGCAGCAACATCCGTTTGCAGGAATGATAGAGTGCGTTCCGTCGTTTACTTCATTAGCTG
TTTACTACAATGTATACGAAGTATGGAAGCGAAATGAGAGAAGTGAAAGGTCACATGATTACGTTCGCCAGTATATAAAG
GAGCTGTGTGATTCTTATAAAGAAGAAGTAAAACAGGATGTGAAACATATCTCTATACCCGTTTGTTACGGGGGAGAATA
TGGACCAGATTTAGAAGAAGTCGCGCATTATCACGGTTTACAAGTAGAAGATGTGATTCGAATACATAGTGAAACTACAT
ATTTTGTATATATGCTAGGTTTTACACCAGGGTTCCCGTATTTAGGAGGACTTTCAAAAGAATTAGAAACACCTAGAAAA
GAAACACCACGGTTACAAATCTCTCCTGGTTCGGTAGGTATTGGCGGAAATCAAACAGGTATATATCCGCTTGAAACACC
AGGAGGATGGAATATTATCGGCCGAACTCCTATTTCATTATTTAATCCGGAAGAAGAAATACCAACATATATTCAAAGTG
GTATGTATTTACGATTTATTCCAATAACGAAGGAAGAGTACGTATCGCTTGAGGGGGCTAAAAAATGGATGTAG

Upstream 100 bases:

>100_bases
ACAACGAAAGTAACATTTCATATTTTATTGCGAAGGTGAAAGAAACAGCGCATCGCATTTCGAAAGAACTCGGCTTTTTG
GCATAGAAAGAGGGAGTAGG

Downstream 100 bases:

>100_bases
AGGTTTTGCATGCGGGAATGTTTACAACAGTCCAAGATTTAGGGCGATCTCATTATCAACAATACGGTGTGCCAGTTGGC
GGGGGGATGGATCAAAGTGC

Product: conserved hypothetical protein TIGR00370

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRFSALGDQAIIVTFGEEIKRDIYEKVQRLFQALQQHPFAGMIECVPSFTSLAVYYNVYEVWKRNERSERSHDYVRQYIK
ELCDSYKEEVKQDVKHISIPVCYGGEYGPDLEEVAHYHGLQVEDVIRIHSETTYFVYMLGFTPGFPYLGGLSKELETPRK
ETPRLQISPGSVGIGGNQTGIYPLETPGGWNIIGRTPISLFNPEEEIPTYIQSGMYLRFIPITKEEYVSLEGAKKWM

Sequences:

>Translated_237_residues
MRFSALGDQAIIVTFGEEIKRDIYEKVQRLFQALQQHPFAGMIECVPSFTSLAVYYNVYEVWKRNERSERSHDYVRQYIK
ELCDSYKEEVKQDVKHISIPVCYGGEYGPDLEEVAHYHGLQVEDVIRIHSETTYFVYMLGFTPGFPYLGGLSKELETPRK
ETPRLQISPGSVGIGGNQTGIYPLETPGGWNIIGRTPISLFNPEEEIPTYIQSGMYLRFIPITKEEYVSLEGAKKWM
>Mature_237_residues
MRFSALGDQAIIVTFGEEIKRDIYEKVQRLFQALQQHPFAGMIECVPSFTSLAVYYNVYEVWKRNERSERSHDYVRQYIK
ELCDSYKEEVKQDVKHISIPVCYGGEYGPDLEEVAHYHGLQVEDVIRIHSETTYFVYMLGFTPGFPYLGGLSKELETPRK
ETPRLQISPGSVGIGGNQTGIYPLETPGGWNIIGRTPISLFNPEEEIPTYIQSGMYLRFIPITKEEYVSLEGAKKWM

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=220, Percent_Identity=38.6363636363636, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 27281; Mature: 27281

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSALGDQAIIVTFGEEIKRDIYEKVQRLFQALQQHPFAGMIECVPSFTSLAVYYNVYE
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VWKRNERSERSHDYVRQYIKELCDSYKEEVKQDVKHISIPVCYGGEYGPDLEEVAHYHGL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEECCCCCCCHHHHHHHCCC
QVEDVIRIHSETTYFVYMLGFTPGFPYLGGLSKELETPRKETPRLQISPGSVGIGGNQTG
CHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHCCCHHHCCEEEECCCCEECCCCCCC
IYPLETPGGWNIIGRTPISLFNPEEEIPTYIQSGMYLRFIPITKEEYVSLEGAKKWM
EEECCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCCC
>Mature Secondary Structure
MRFSALGDQAIIVTFGEEIKRDIYEKVQRLFQALQQHPFAGMIECVPSFTSLAVYYNVYE
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VWKRNERSERSHDYVRQYIKELCDSYKEEVKQDVKHISIPVCYGGEYGPDLEEVAHYHGL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEECCCCCCCHHHHHHHCCC
QVEDVIRIHSETTYFVYMLGFTPGFPYLGGLSKELETPRKETPRLQISPGSVGIGGNQTG
CHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHCCCHHHCCEEEECCCCEECCCCCCC
IYPLETPGGWNIIGRTPISLFNPEEEIPTYIQSGMYLRFIPITKEEYVSLEGAKKWM
EEECCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]