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The map label for this gene is ilvB2 [H]

Identifier: 218235285

GI number: 218235285

Start: 1756510

End: 1758231

Strand: Direct

Name: ilvB2 [H]

Synonym: BCB4264_A1851

Alternate gene names: 218235285

Gene position: 1756510-1758231 (Clockwise)

Preceding gene: 218235646

Following gene: 218234338

Centisome position: 32.41

GC content: 39.78

Gene sequence:

>1722_bases
ATGGAAATGAAGCAGCAGTATACAGCGTATGAGAAGTTACAGTATGAAGAAATGACAGGCGCTGGGCACGTGATTCAATG
TTTGAAGAAATTGGGTGTAACGACCGTTTTCGGCTATCCAGGCGGAGCGATTTTACCAGTATACGATGCGTTATACGAAA
GTGGTTTGAAACATGTTTTAACTCGTCATGAGCAAGCTGCCATTCATGCGGCTGAAGGATATGCGAGAGCTTCTAGAAAG
GTCGGGGTAGTCTTTGCTACCTCTGGCCCAGGGGCGACAAATTTAGTTACGGGCTTAGCAGACGCTTATATGGATTCGAT
TCCTTTAGTTGTCATTACCGGTCAAGTTGCGACGCCTTTAATTGGTAAAGATGGATTTCAAGAAGCGGATGTTGTCGGAA
TTACAGTACCTGTAACGAAGCATAATTACCAAGTTCGTGATGTAAATCATGTATCACAAGTTGTGCAAGAAGCTTTTTAC
ATCGCCAAAAGCGGACGACCGGGACCAGTATTAATCGATATTCCAAAAGATGTTCAAAATGCAAAAGTCACCAGTTTCTT
TAATGAAGAAGTTGATATTCCGGGGTACAAACCAGAACTTGTACCAGACAGCATGAAACTTAGAGAGGTGGCTAAAGCAA
TTTCAAAATCAAAGCGTCCACTTCTTTATATTGGAGGAGGTGTCATTCATTCAGGTGGATCCGATGAACTCTTCGAATTC
GCAAGGGAGAACCGTATTCCAGTCGTTTCAACTTTAATGGGACTTGGTGCATATCCACCGGGAGATCCATTGTTTCTAGG
GATGCTTGGTATGCATGGGACATACGCTGCTAATATGGCGGTAACAGAATGTGATTTATTACTTGCATTAGGTGTTCGCT
TCGATGATCGTGTAACAGGAAAATTAGAACTTTTTTCTCCGCACTCGAAAAAAGTACATATTGATATTGATCCTTCTGAG
TTTCATAAAAATATAACTGTAGAGCATCCGATTGTTGGTGATGTGAAAAAAGCGTTACACATGTTGTTACATATGTCTAT
TTATACACAAACAGACGAATGGCTTCAGAAAGTAAAGGCATGGAAAGAAGAATATCCACTTTCTTATAAGCAAAAAGAAT
CTGAGTTAAAACCACAGCATGTGATCAACTTAGTAAGTGAATTAACGAATGGTGAAGCGATTGTCACAACAGAAGTAGGA
CAGCATCAAATGTGGGCTGCTCACTTCTATAAAGCAAGAAAACCTCGGACGTTCCTTACTTCTGGAGGATTAGGAACGAT
GGGGTTTGGTTTTCCAGCAGCAATCGGTGCTCAGCTAGCTAAAAAAGAAGAACTTGTTATTTGTATTGCAGGTGATGCTT
CTTTTCAAATGAACATTCAAGAACTACAAACAATTGCTGAAAATAATATCCCTGTAAAAGTATTTATCATAAATAACAAA
TTTTTAGGGATGGTAAGGCAATGGCAAGAAATGTTTTATGAAAATCGTTTATCAGAGTCAAAAATTGGATCACCAGATTT
TGTGAAAGTAGCAGAAGCTTATGGAGTAAAGGGATTAAGAGCAACAAATTCAACCGAGGCGAAACAAGTGATGTTAGAAG
CATTTGCTCACGAAGGTCCTGTCGTAGTTGATTTTTGTGTAGAAGAAGGTGAAAACGTATTTCCGATGGTTCCGCCAAAC
AAAGGAAATAACGAAATGATTATGAAGAGGTGGGAAGAATGA

Upstream 100 bases:

>100_bases
ACGAAACAATTGACCGAAGAATTTAAAAAATTAACGAGAGAAAGAGGAGTACGTGTTCCTGGGCTGACGGAAAGCTTAGC
ATAAATAAGGAGAGGAGTTA

Downstream 100 bases:

>100_bases
GTCATACTTTTTCACTCGTTATTCATAACGAGCCAAGTGTCTTATTACGTATAAGTGGGATTTTTGCTCGGCGTGGTTAT
TATATTTCTTCTTTACATTT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEMKQQYTAYEKLQYEEMTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHVLTRHEQAAIHAAEGYARASRK
VGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNHVSQVVQEAFY
IAKSGRPGPVLIDIPKDVQNAKVTSFFNEEVDIPGYKPELVPDSMKLREVAKAISKSKRPLLYIGGGVIHSGGSDELFEF
ARENRIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSE
FHKNITVEHPIVGDVKKALHMLLHMSIYTQTDEWLQKVKAWKEEYPLSYKQKESELKPQHVINLVSELTNGEAIVTTEVG
QHQMWAAHFYKARKPRTFLTSGGLGTMGFGFPAAIGAQLAKKEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNK
FLGMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPN
KGNNEMIMKRWEE

Sequences:

>Translated_573_residues
MEMKQQYTAYEKLQYEEMTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHVLTRHEQAAIHAAEGYARASRK
VGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNHVSQVVQEAFY
IAKSGRPGPVLIDIPKDVQNAKVTSFFNEEVDIPGYKPELVPDSMKLREVAKAISKSKRPLLYIGGGVIHSGGSDELFEF
ARENRIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSE
FHKNITVEHPIVGDVKKALHMLLHMSIYTQTDEWLQKVKAWKEEYPLSYKQKESELKPQHVINLVSELTNGEAIVTTEVG
QHQMWAAHFYKARKPRTFLTSGGLGTMGFGFPAAIGAQLAKKEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNK
FLGMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPN
KGNNEMIMKRWEE
>Mature_573_residues
MEMKQQYTAYEKLQYEEMTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHVLTRHEQAAIHAAEGYARASRK
VGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNHVSQVVQEAFY
IAKSGRPGPVLIDIPKDVQNAKVTSFFNEEVDIPGYKPELVPDSMKLREVAKAISKSKRPLLYIGGGVIHSGGSDELFEF
ARENRIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPHSKKVHIDIDPSE
FHKNITVEHPIVGDVKKALHMLLHMSIYTQTDEWLQKVKAWKEEYPLSYKQKESELKPQHVINLVSELTNGEAIVTTEVG
QHQMWAAHFYKARKPRTFLTSGGLGTMGFGFPAAIGAQLAKKEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNK
FLGMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPN
KGNNEMIMKRWEE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=25.503355704698, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=24.2369838420108, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=46.8531468531469, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=43.5626102292769, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=553, Percent_Identity=34.5388788426763, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=27.4725274725275, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=23.2974910394265, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=552, Percent_Identity=27.8985507246377, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=552, Percent_Identity=27.8985507246377, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=586, Percent_Identity=24.4027303754266, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=45.6445993031359, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320816, Length=481, Percent_Identity=25.5717255717256, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321524, Length=499, Percent_Identity=23.2464929859719, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=471, Percent_Identity=26.3269639065817, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63286; Mature: 63286

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKQQYTAYEKLQYEEMTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHVL
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
TRHEQAAIHAAEGYARASRKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPL
HHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
IGKDGFQEADVVGITVPVTKHNYQVRDVNHVSQVVQEAFYIAKSGRPGPVLIDIPKDVQN
CCCCCCCCCCEEEEEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
AKVTSFFNEEVDIPGYKPELVPDSMKLREVAKAISKSKRPLLYIGGGVIHSGGSDELFEF
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEEEECCCCHHHHHH
ARENRIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTG
HHHCCCCHHHHHHHCCCCCCCCCEEEEHHCCCCCHHCCEEHHHHHHHHHHCCCCCCCCCC
KLELFSPHSKKVHIDIDPSEFHKNITVEHPIVGDVKKALHMLLHMSIYTQTDEWLQKVKA
EEEEECCCCCEEEEECCHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
WKEEYPLSYKQKESELKPQHVINLVSELTNGEAIVTTEVGQHQMWAAHFYKARKPRTFLT
HHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEE
SGGLGTMGFGFPAAIGAQLAKKEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNK
CCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEECHHHHHHHHCCCCCEEEEEECCH
FLGMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGP
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
VVVDFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEEEEECCCCCEECCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MEMKQQYTAYEKLQYEEMTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHVL
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
TRHEQAAIHAAEGYARASRKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPL
HHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
IGKDGFQEADVVGITVPVTKHNYQVRDVNHVSQVVQEAFYIAKSGRPGPVLIDIPKDVQN
CCCCCCCCCCEEEEEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
AKVTSFFNEEVDIPGYKPELVPDSMKLREVAKAISKSKRPLLYIGGGVIHSGGSDELFEF
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEEEECCCCHHHHHH
ARENRIPVVSTLMGLGAYPPGDPLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTG
HHHCCCCHHHHHHHCCCCCCCCCEEEEHHCCCCCHHCCEEHHHHHHHHHHCCCCCCCCCC
KLELFSPHSKKVHIDIDPSEFHKNITVEHPIVGDVKKALHMLLHMSIYTQTDEWLQKVKA
EEEEECCCCCEEEEECCHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
WKEEYPLSYKQKESELKPQHVINLVSELTNGEAIVTTEVGQHQMWAAHFYKARKPRTFLT
HHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEE
SGGLGTMGFGFPAAIGAQLAKKEELVICIAGDASFQMNIQELQTIAENNIPVKVFIINNK
CCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEECHHHHHHHHCCCCCEEEEEECCH
FLGMVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGP
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
VVVDFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEEEEECCCCCEECCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]