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The map label for this gene is ilvE2 [H]

Identifier: 218235646

GI number: 218235646

Start: 1755594

End: 1756493

Strand: Direct

Name: ilvE2 [H]

Synonym: BCB4264_A1850

Alternate gene names: 218235646

Gene position: 1755594-1756493 (Clockwise)

Preceding gene: 218232004

Following gene: 218235285

Centisome position: 32.4

GC content: 37.44

Gene sequence:

>900_bases
ATGGGAAACCAGTACATTTACATGAATGGGGAATTTGTAGAAAAAGAAAAGGCAGTTGTTTCAGTGTATGATCATGGTTT
TTTATACGGAGACGGTGTATTTGAAGGGATTCGTAGTTACGGAGGAAATGTATTTTGTTTAAAAGAACATGTGAAACGAT
TATATGAATCAGCGAAATCTATTTTACTAACAATTCCAATGACGGTAGAAGAAATGGAAGAAGCAGTTTTACATACACTC
CAAAAAAATGAATACGCTGATGCTTACATTCGCTTAATTGTCTCAAGAGGAAAAGGTGACTTAGGGCTCGATCCGAGAAG
TTGTGTGAAGCCGAGCGTAATTATCATTGCAGAACAATTAAAGTTATTCCCACAAGAATTTTATGATAATGGGCTAAGCG
TTGTATCTGTTGCATCAAGACGTAATACGCCAGACGCGTTAGATCCACGTATTAAGTCAATGAATTATTTAAATAACGTA
CTTGTAAAAATTGAAGCAGCACAAGCAGGAGTGTTAGAGGCTCTTATGTTAAATCAACAAGGATATGTTTGTGAAGGTTC
TGGCGATAATGTTTTCGTTGTGAAAGATGGAAAAGTATTAACCCCGCCATCCTATTTAGGAGCATTAGAAGGTATTACGA
GAAATAGTGTTATCGAGCTATGTGAACGACTGAATATTCCGTGTGAGGAAAGACCATTCACTCGCCATGATGTATATGTA
GCGGATGAAGTATTTTTAACAGGAACAGCAGCAGAGGTAATTCCAGTTGTAAAAGTAGATTCGAGAGAAATTGGAGATGG
GAAACCAGGAAGTGTAACGAAACAATTGACCGAAGAATTTAAAAAATTAACGAGAGAAAGAGGAGTACGTGTTCCTGGGC
TGACGGAAAGCTTAGCATAA

Upstream 100 bases:

>100_bases
AAGGGTTATATACTGAATATTCTGTTAAAAATACTCATCTCAGCAATCGATGTAGAAATAGATTGCAAAAAATAGAGAAA
AAAAAGAGGAGCGATGTGAG

Downstream 100 bases:

>100_bases
ATAAGGAGAGGAGTTAATGGAAATGAAGCAGCAGTATACAGCGTATGAGAAGTTACAGTATGAAGAAATGACAGGCGCTG
GGCACGTGATTCAATGTTTG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MGNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPMTVEEMEEAVLHTL
QKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNV
LVKIEAAQAGVLEALMLNQQGYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLNIPCEERPFTRHDVYV
ADEVFLTGTAAEVIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLTESLA

Sequences:

>Translated_299_residues
MGNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPMTVEEMEEAVLHTL
QKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNV
LVKIEAAQAGVLEALMLNQQGYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLNIPCEERPFTRHDVYV
ADEVFLTGTAAEVIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLTESLA
>Mature_298_residues
GNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPMTVEEMEEAVLHTLQ
KNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNVL
VKIEAAQAGVLEALMLNQQGYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLNIPCEERPFTRHDVYVA
DEVFLTGTAAEVIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLTESLA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=290, Percent_Identity=38.6206896551724, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1787338, Length=241, Percent_Identity=25.3112033195021, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33158; Mature: 33027

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKS
CCCCEEEECCCEECCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
ILLTIPMTVEEMEEAVLHTLQKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQL
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEHHH
KLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNVLVKIEAAQAGVLEALMLNQQ
HHHHHHHHCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCC
GYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLNIPCEERPFTRHDVYV
CCEEECCCCEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
ADEVFLTGTAAEVIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLTESLA
ECEEEEECCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCC
>Mature Secondary Structure 
GNQYIYMNGEFVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKS
CCCEEEECCCEECCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
ILLTIPMTVEEMEEAVLHTLQKNEYADAYIRLIVSRGKGDLGLDPRSCVKPSVIIIAEQL
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEHHH
KLFPQEFYDNGLSVVSVASRRNTPDALDPRIKSMNYLNNVLVKIEAAQAGVLEALMLNQQ
HHHHHHHHCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCC
GYVCEGSGDNVFVVKDGKVLTPPSYLGALEGITRNSVIELCERLNIPCEERPFTRHDVYV
CCEEECCCCEEEEEECCEEECCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
ADEVFLTGTAAEVIPVVKVDSREIGDGKPGSVTKQLTEEFKKLTRERGVRVPGLTESLA
ECEEEEECCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]