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The map label for this gene is thiM [H]

Identifier: 218234883

GI number: 218234883

Start: 423176

End: 423985

Strand: Direct

Name: thiM [H]

Synonym: BCB4264_A0439

Alternate gene names: 218234883

Gene position: 423176-423985 (Clockwise)

Preceding gene: 218231308

Following gene: 218236157

Centisome position: 7.81

GC content: 39.75

Gene sequence:

>810_bases
ATGAATCTGAAAGAATTTAGTAAAGTAGTGGATTTGGTGAGAGAATCTAATCCGCTTGTTCATAATATTACAAATGTTGT
TGTAACAAATTTTACTGCTAACGGTTTGTTGGCACTTGGGGCATCGCCTGTAATGGCGTATGCAAAAGAAGAAGTAGCAG
AAATGGCTAGCATTGCTGGAGCGTTAGTATTAAATATGGGGACACTTCGTCCTGAAGAAGTAGAAGCGATGTTACTCGCG
GGTAAATCAGCAAATGCGAACAATGTGCCAGTACTATTTGATCCAGTTGGTGCAGGAGCAACTTCGTATCGAACAGAAGT
TGCGAGACATATTCCGGCTGAAATCGATTTAGCAATTATCCGCGGAAATGCAGCTGAAATAGCAAATGTTATTAATGAGA
AATGGGAAATTAAAGGGGTAGACGCTGGTACTGGAAATGGCGATGTTGTAAGTATTGCAAGGCAGGCAGCAGATGAACTA
AATACAGTTGCGGTCATTACTGGAAAAGAAGATGTTGTTACAGATGGAGAGAGAACTATTGTTATTCGAAATGGTCACCC
TATTTTAACGAAAGTTACGGGAACAGGTTGTTTACTAACTTCTGTAATAGGAGCATTTGTAGCAGTGGAAAAAGATTATG
TAAAAGCAGCAGTAGCAGCATTAACGTTTTATGGTGTAGCTTCGGAACTGGCAGCTGCTAAGACCGTGGAAAAGGGACCT
GGTAGTTTCCAAATTGAATTTTTAAATCAGTTAGCGAATACTACTTCTGGTGATATTGAGAAGTATGGAAAGATTGAGGA
GTTAGAGTAA

Upstream 100 bases:

>100_bases
CAGCGTAGGGAGAGTGTAAAAGCACATGTTCAGGCACTTTGCGTACGCGCAAAGTGTCTTTTTGTGTATCTCCCATCATT
ATAGTTAGGAGATGAAAAGG

Downstream 100 bases:

>100_bases
GAGGGAGTGAAAGAAATGGCACGCATTGAAATAGATAAAATGTCTAAGCTATTGCAAGTGTATTTTATTATGGGAAGTAA
TAACTGCACGAGGGATCCTT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNLKEFSKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPEEVEAMLLA
GKSANANNVPVLFDPVGAGATSYRTEVARHIPAEIDLAIIRGNAAEIANVINEKWEIKGVDAGTGNGDVVSIARQAADEL
NTVAVITGKEDVVTDGERTIVIRNGHPILTKVTGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVASELAAAKTVEKGP
GSFQIEFLNQLANTTSGDIEKYGKIEELE

Sequences:

>Translated_269_residues
MNLKEFSKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPEEVEAMLLA
GKSANANNVPVLFDPVGAGATSYRTEVARHIPAEIDLAIIRGNAAEIANVINEKWEIKGVDAGTGNGDVVSIARQAADEL
NTVAVITGKEDVVTDGERTIVIRNGHPILTKVTGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVASELAAAKTVEKGP
GSFQIEFLNQLANTTSGDIEKYGKIEELE
>Mature_269_residues
MNLKEFSKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPEEVEAMLLA
GKSANANNVPVLFDPVGAGATSYRTEVARHIPAEIDLAIIRGNAAEIANVINEKWEIKGVDAGTGNGDVVSIARQAADEL
NTVAVITGKEDVVTDGERTIVIRNGHPILTKVTGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVASELAAAKTVEKGP
GSFQIEFLNQLANTTSGDIEKYGKIEELE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=254, Percent_Identity=39.7637795275591, Blast_Score=164, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6325042, Length=273, Percent_Identity=34.4322344322344, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28250; Mature: 28250

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKEFSKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAG
CCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNMGTLRPEEVEAMLLAGKSANANNVPVLFDPVGAGATSYRTEVARHIPAEIDLAII
HHHHHCCCCCHHHHHEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEE
RGNAAEIANVINEKWEIKGVDAGTGNGDVVSIARQAADELNTVAVITGKEDVVTDGERTI
ECCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEECCCEEE
VIRNGHPILTKVTGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVASELAAAKTVEKGP
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSFQIEFLNQLANTTSGDIEKYGKIEELE
CCEEHHHHHHHHCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MNLKEFSKVVDLVRESNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAG
CCHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNMGTLRPEEVEAMLLAGKSANANNVPVLFDPVGAGATSYRTEVARHIPAEIDLAII
HHHHHCCCCCHHHHHEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEE
RGNAAEIANVINEKWEIKGVDAGTGNGDVVSIARQAADELNTVAVITGKEDVVTDGERTI
ECCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEECCCEEE
VIRNGHPILTKVTGTGCLLTSVIGAFVAVEKDYVKAAVAALTFYGVASELAAAKTVEKGP
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GSFQIEFLNQLANTTSGDIEKYGKIEELE
CCEEHHHHHHHHCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA