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The map label for this gene is thiE

Identifier: 218236157

GI number: 218236157

Start: 424001

End: 424660

Strand: Direct

Name: thiE

Synonym: BCB4264_A0440

Alternate gene names: 218236157

Gene position: 424001-424660 (Clockwise)

Preceding gene: 218234883

Following gene: 218234291

Centisome position: 7.82

GC content: 38.48

Gene sequence:

>660_bases
ATGGCACGCATTGAAATAGATAAAATGTCTAAGCTATTGCAAGTGTATTTTATTATGGGAAGTAATAACTGCACGAGGGA
TCCTTTAGCTGTATTGAAAGAAGCGTTAGATGGTGGAGTGACAATTTTTCAGTTTCGCGAGAAGGGGGAAGGTTCTTTAA
TCGGAGAAGATAGAGTGCGCTTTGCAAAAGAATTGCAGACGCTTTGTAAGGAATATAGTGTTCCTTTCATTGTAAATGAT
GATGTAGAATTAGCCATTGAATTAGATGCAGATGGTGTTCATGTAGGACAAGATGATGAAGGAATTACATCTGTTCGTGA
AAAAATGGGAGATAAAATTATCGGCGTATCTGCTCATACAATTGAAGAAGCACGCTTTGCAATCGAAAATGGAGCAGATT
ATTTAGGCGTTGGACCTATTTTCCCAACGAGTACGAAAAAAGATACGAAAGCGGTTCAAGGAACGAAAGGATTAGCCTAT
TTTAGAGAACAAGGAATTACAGTGCCAATCGTTGGTATTGGTGGGATTACAATTGAAAATACAGCGGCAGTTATAGAAGC
GGGTGCGGACGGTGTTTCAGTTATTTCAGCAATTAGTTTAGCTGAATCTGCGTATGAAAGTACGAGAAAATTAGCTGAAG
AAGTAAAGAGAAGTTTGTAG

Upstream 100 bases:

>100_bases
TTTCCAAATTGAATTTTTAAATCAGTTAGCGAATACTACTTCTGGTGATATTGAGAAGTATGGAAAGATTGAGGAGTTAG
AGTAAGAGGGAGTGAAAGAA

Downstream 100 bases:

>100_bases
GGAAGGACGTGTAGTATTTTAAGTGCTACATGGAGAAATAGACTGCCCCAAAATTTAAACAAACATTTGATTAAAAAAGC
ACAAACCTTTTTAAATAAAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MARIEIDKMSKLLQVYFIMGSNNCTRDPLAVLKEALDGGVTIFQFREKGEGSLIGEDRVRFAKELQTLCKEYSVPFIVND
DVELAIELDADGVHVGQDDEGITSVREKMGDKIIGVSAHTIEEARFAIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAY
FREQGITVPIVGIGGITIENTAAVIEAGADGVSVISAISLAESAYESTRKLAEEVKRSL

Sequences:

>Translated_219_residues
MARIEIDKMSKLLQVYFIMGSNNCTRDPLAVLKEALDGGVTIFQFREKGEGSLIGEDRVRFAKELQTLCKEYSVPFIVND
DVELAIELDADGVHVGQDDEGITSVREKMGDKIIGVSAHTIEEARFAIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAY
FREQGITVPIVGIGGITIENTAAVIEAGADGVSVISAISLAESAYESTRKLAEEVKRSL
>Mature_218_residues
ARIEIDKMSKLLQVYFIMGSNNCTRDPLAVLKEALDGGVTIFQFREKGEGSLIGEDRVRFAKELQTLCKEYSVPFIVNDD
VELAIELDADGVHVGQDDEGITSVREKMGDKIIGVSAHTIEEARFAIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAYF
REQGITVPIVGIGGITIENTAAVIEAGADGVSVISAISLAESAYESTRKLAEEVKRSL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=188, Percent_Identity=33.5106382978723, Blast_Score=85, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325042, Length=196, Percent_Identity=38.265306122449, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_BACC4 (B7H7H5)

Other databases:

- EMBL:   CP001176
- RefSeq:   YP_002365219.1
- ProteinModelPortal:   B7H7H5
- EnsemblBacteria:   EBBACT00000110551
- GeneID:   7100820
- GenomeReviews:   CP001176_GR
- KEGG:   bcb:BCB4264_A0440
- GeneTree:   EBGT00050000001882
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   PRK00043
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23601; Mature: 23470

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIEIDKMSKLLQVYFIMGSNNCTRDPLAVLKEALDGGVTIFQFREKGEGSLIGEDRVR
CCCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCEECHHHHH
FAKELQTLCKEYSVPFIVNDDVELAIELDADGVHVGQDDEGITSVREKMGDKIIGVSAHT
HHHHHHHHHHHCCCCEEECCCEEEEEEECCCCEECCCCCCHHHHHHHHHCCEEEECCHHH
IEEARFAIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAYFREQGITVPIVGIGGITIEN
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCCCCHHHHHHHCCCEEEEEECCCEEECC
TAAVIEAGADGVSVISAISLAESAYESTRKLAEEVKRSL
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARIEIDKMSKLLQVYFIMGSNNCTRDPLAVLKEALDGGVTIFQFREKGEGSLIGEDRVR
CCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCEECHHHHH
FAKELQTLCKEYSVPFIVNDDVELAIELDADGVHVGQDDEGITSVREKMGDKIIGVSAHT
HHHHHHHHHHHCCCCEEECCCEEEEEEECCCCEECCCCCCHHHHHHHHHCCEEEECCHHH
IEEARFAIENGADYLGVGPIFPTSTKKDTKAVQGTKGLAYFREQGITVPIVGIGGITIEN
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCCCCHHHHHHHCCCEEEEEECCCEEECC
TAAVIEAGADGVSVISAISLAESAYESTRKLAEEVKRSL
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA