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The map label for this gene is leuC [H]

Identifier: 218234850

GI number: 218234850

Start: 1397376

End: 1398770

Strand: Direct

Name: leuC [H]

Synonym: BCB4264_A1455

Alternate gene names: 218234850

Gene position: 1397376-1398770 (Clockwise)

Preceding gene: 218231765

Following gene: 218236062

Centisome position: 25.79

GC content: 41.72

Gene sequence:

>1395_bases
ATGGGGAAAAGGTTACTAGATAAGCTTTGGGAAAGACATGTAGTTGCGACTAATGAAAATGGATTGGATTTATTATATAT
CGATCTACATCTTGTTCATGAAGTAACGTCACCGCAAGCCTTTGAAGGCTTGCGGCTTACAAATCGAACAGTCCGCAGAC
CAGATTTAACATTTGCAACGATGGATCATAATATTCCAACGAAAGATGTTTGGAATATTACCGACCGCATTGCGAAGCAG
CAACTAGATACACTTCGGGAAAACTGTAAACAATTTCAGGTGCCATTAGCGGATATCGGAGATGAAGAGCAAGGGATCGT
TCATGTAATAGGACCAGAACTTGGACTCACACAGCCAGGAAAAACGATTGTTTGTGGTGATAGTCATACAGCAACACACG
GTGCTTTTGGCGCACTAGCGTTTGGTATTGGTACGAGTGAAGTGGAACATGTATTGGCAACGCAAACGTTATGGCAACGA
AAGCCGAAAGCGATGGGCATTGAGTTAAAAGGAAAGTTACCGCAAGGTGTCTATGCGAAAGATATTATTTTACACCTTCT
TTCAAAGTACGGAGTAGCAGTTGGAACAGGTTACGTAATGGAATTTTACGGAGAGGCCATTCATGCTATGGATATGGAAG
AGAGGATGACGCTTTGCAATATGGCAATTGAAGGGGGAGCAAAGGCTGGCATTATTGCACCAGATGAAAAAACGTTTGCT
TACGTAAAAGGGCGTAAATATGCACCGAAAGACTATGAATCTATTAAGAAAAAATGGTCAGAACTTTATACAGATTTAGA
TGTAGTTTATGATTTACATATTCTAGTAGATGTTACGGATTTAGCGCCGTACGTTACGTGGGGAACAAATCCTAGTATGG
GTGTTCGGATCGATGAGAAATTGCCAGAAAAGCATGATGCAAATGATGAAAGAGCGTTTTCTTATATGGGATTGAGTCCC
GGACAAAGTACGTATGACATTCCAGTTCAACATGTTTTCATTGGATCTTGTACAAATTCCAGGCTTTCTGACTTAGAAAT
TGCTGCATCTGTCGTGAAAGGTAAAAAAGTAAAAGAAGGTGTGCGAGCACTTGTTGTACCTGGATCTCAAAGGGTAAGAG
AAGCAGCGATGCATAAAGGATTACATCGCATATTTGAAGAAGCTGGATTTGAATGGAGAGAGCCCGGATGCTCAATGTGT
CTAGGTATGAATCCAGATCAAGTGCCTGAAGGAGAACATTGTGCCTCTACGTCCAATCGCAATTTTGAAGGAAGGCAAGG
AAAGGGAGCACGAACGCATTTAGTTAGCCCGGCAATGGCGGCAGCCGCTGCGTTATATGGTCATTTTGTTGATATTAGAA
AGGAGAGTTATGATGGAGCCATTTCGTATTCATAA

Upstream 100 bases:

>100_bases
AATGTACAGCAGATATCGGGGGGACTGAAACGACAACTTCATTTACAAAGGCAGTTATTCAAGAAATGGAAGAGCAAGCG
TTAGTAGGGAGAGGGAGATA

Downstream 100 bases:

>100_bases
AGGTACTGCCGCAGTACTGATGAATGATAACATTGATACAGATCAAATTATTCCGAAGCAATATTTAAAGAGAATTGAAA
GAACTGGGTTTGGGAAGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLPQGVYAKDIILHLLSKYGVAVGTGYVMEFYGEAIHAMDMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPKDYESIKKKWSELYTDLDVVYDLHILVDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDANDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEGVRALVVPGSQRVREAAMHKGLHRIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS

Sequences:

>Translated_464_residues
MGKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLPQGVYAKDIILHLLSKYGVAVGTGYVMEFYGEAIHAMDMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPKDYESIKKKWSELYTDLDVVYDLHILVDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDANDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEGVRALVVPGSQRVREAAMHKGLHRIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS
>Mature_463_residues
GKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQQ
LDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRK
PKAMGIELKGKLPQGVYAKDIILHLLSKYGVAVGTGYVMEFYGEAIHAMDMEERMTLCNMAIEGGAKAGIIAPDEKTFAY
VKGRKYAPKDYESIKKKWSELYTDLDVVYDLHILVDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDANDERAFSYMGLSPG
QSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEGVRALVVPGSQRVREAAMHKGLHRIFEEAGFEWREPGCSMCL
GMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=353, Percent_Identity=25.4957507082153, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI8659555, Length=384, Percent_Identity=27.6041666666667, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI41352693, Length=377, Percent_Identity=25.4641909814324, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=58.7096774193548, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87081781, Length=343, Percent_Identity=24.7813411078717, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI2367097, Length=337, Percent_Identity=22.8486646884273, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=359, Percent_Identity=30.3621169916435, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25149337, Length=359, Percent_Identity=30.3621169916435, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=29.8360655737705, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=431, Percent_Identity=26.2180974477958, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=55.9322033898305, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=28.5024154589372, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6322261, Length=403, Percent_Identity=28.287841191067, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=357, Percent_Identity=29.6918767507003, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.2251308900524, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=27.3657289002558, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=27.3657289002558, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571643, Length=390, Percent_Identity=27.9487179487179, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=28.6842105263158, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137564, Length=380, Percent_Identity=26.0526315789474, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51353; Mature: 51222

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CCHHHHHHHHHCCEEEECCCCCEEEEEEEEHHHHHCCCHHHCCCHHCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLPQGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGEAIHAMDMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPKDYESIKKKWSELYTDLDVVYDLHILVDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCEEEECCCCCCCEEEHHH
LPEKHDANDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHC
VRALVVPGSQRVREAAMHKGLHRIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CHHHHHHHHHCCEEEECCCCCEEEEEEEEHHHHHCCCHHHCCCHHCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLPQGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGEAIHAMDMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPKDYESIKKKWSELYTDLDVVYDLHILVDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCEEEECCCCCCCEEEHHH
LPEKHDANDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGKKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHC
VRALVVPGSQRVREAAMHKGLHRIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA