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The map label for this gene is leuB [H]

Identifier: 218231765

GI number: 218231765

Start: 1396312

End: 1397376

Strand: Direct

Name: leuB [H]

Synonym: BCB4264_A1454

Alternate gene names: 218231765

Gene position: 1396312-1397376 (Clockwise)

Preceding gene: 218231353

Following gene: 218234850

Centisome position: 25.77

GC content: 41.13

Gene sequence:

>1065_bases
ATGGAAAAACGTATCGTTTGTTTAGCTGGTGATGGTGTCGGTCCAGAAGTAATGGAGAGTGCGAAGGAAGTATTGCATAT
GGTAGAAAGGCTATATGGACATCATTTTCATTTGCAAGATGAGTACTTTGGTGGTGTTGCTATCGATTTAACTGGGCAAC
CATTACCACAGCGAACACTTGCCGCCTGTTTAGCGAGTGATGCGGTTTTACTTGGAGCAGTAGGTGGACCAAGGTGGGAT
AGTGCGAAAGAAAGGCCTGAGAAAGGATTATTAGCATTAAGAAAAGGACTCGGTGTATTTGCGAATGTTCGCCCTGTAAC
GGTAGAAAGTGCAACTGCACATTTATCACCATTAAAAAACGCTGATGAAATTGATTTCGTTGTCGTTCGTGAATTAACAG
GCGGAATTTATTTTTCTTATCCGAAAGAGCGAACGGAAGAAGTGGCGACAGATACACTTACGTATCATCGCCATGAAATT
GAACGTATCGTTTCGTATGCTTTTCAATTAGCAAATAAGCGGAAGAAAAAAGTAACATCTATTGATAAAGCGAATGTTTT
AGAGTCTAGTAAATTATGGAGAACTGTGACAGAAGAAGTAGCACTTCGCTACCCAAATGTGGAAGTAGAACACATTTTAG
TAGATGCAGCAGCTATGGAATTAATTCGGAATCCAGGAAGATTTGACGTTATTGTAACGGAAAATTTATTTGGTGATATT
TTAAGTGATGAGGCTTCAGTATTAGCTGGATCATTAGGAATGCTTCCATCAGCAAGTCACGCTGAAAAGGGCCCTTCATT
ATACGAGCCTATTCACGGATCGGCACCGGATATTGCGGGGAAAAATAAGGCAAATCCAATTGCGATGATGCGTTCTGTTG
CAATGATGCTTGGGCAATCGTTCGGATTAACGAGAGAAGGATATGCAATTGAAGAAGCAGTTTCTGCAGTCCTTAAATCA
GGAAAATGTACAGCAGATATCGGGGGGACTGAAACGACAACTTCATTTACAAAGGCAGTTATTCAAGAAATGGAAGAGCA
AGCGTTAGTAGGGAGAGGGAGATAA

Upstream 100 bases:

>100_bases
CTCTTATAGCGCTTGTGAAATAAATAATTGATAGGTGAGAGAAGCTTTCCTCACCTATCAAATTAAAGTTCTGAAAATTC
GGTTAAAAGGAGTGTTAAAA

Downstream 100 bases:

>100_bases
TGGGGAAAAGGTTACTAGATAAGCTTTGGGAAAGACATGTAGTTGCGACTAATGAAAATGGATTGGATTTATTATATATC
GATCTACATCTTGTTCATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
SAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKNADEIDFVVVRELTGGIYFSYPKERTEEVATDTLTYHRHEI
ERIVSYAFQLANKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPNVEVEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGYAIEEAVSAVLKS
GKCTADIGGTETTTSFTKAVIQEMEEQALVGRGR

Sequences:

>Translated_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
SAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKNADEIDFVVVRELTGGIYFSYPKERTEEVATDTLTYHRHEI
ERIVSYAFQLANKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPNVEVEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGYAIEEAVSAVLKS
GKCTADIGGTETTTSFTKAVIQEMEEQALVGRGR
>Mature_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
SAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKNADEIDFVVVRELTGGIYFSYPKERTEEVATDTLTYHRHEI
ERIVSYAFQLANKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPNVEVEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGYAIEEAVSAVLKS
GKCTADIGGTETTTSFTKAVIQEMEEQALVGRGR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=30.6590257879656, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=31.6239316239316, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=31.8840579710145, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI4758582, Length=255, Percent_Identity=32.5490196078431, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=35.5670103092784, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI28178838, Length=230, Percent_Identity=32.1739130434783, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI87081683, Length=333, Percent_Identity=49.8498498498498, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=35.3932584269663, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787381, Length=394, Percent_Identity=24.3654822335025, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=31.3559322033898, Blast_Score=160, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.9659090909091, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=27.4011299435028, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=27.0893371757925, Blast_Score=111, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=48.3240223463687, Blast_Score=292, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.6946778711485, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=345, Percent_Identity=34.4927536231884, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=30.028328611898, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=32.183908045977, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=32.183908045977, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=30.7471264367816, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=30.7471264367816, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24661184, Length=265, Percent_Identity=31.6981132075472, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=27.8260869565217, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI161078633, Length=345, Percent_Identity=27.8260869565217, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24650122, Length=345, Percent_Identity=27.8260869565217, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078637, Length=345, Percent_Identity=27.8260869565217, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078639, Length=342, Percent_Identity=28.0701754385965, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20130355, Length=343, Percent_Identity=23.6151603498542, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38473; Mature: 38473

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDSAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKN
HHHHHCCCEEEECCCCCCCCCHHHCHHHHHHHHHHCCCCEECCCEEEECCHHHHHCCCCC
ADEIDFVVVRELTGGIYFSYPKERTEEVATDTLTYHRHEIERIVSYAFQLANKRKKKVTS
CCCCCEEEEEEHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTVTEEVALRYPNVEVEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FGLTREGYAIEEAVSAVLKSGKCTADIGGTETTTSFTKAVIQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDSAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKN
HHHHHCCCEEEECCCCCCCCCHHHCHHHHHHHHHHCCCCEECCCEEEECCHHHHHCCCCC
ADEIDFVVVRELTGGIYFSYPKERTEEVATDTLTYHRHEIERIVSYAFQLANKRKKKVTS
CCCCCEEEEEEHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTVTEEVALRYPNVEVEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FGLTREGYAIEEAVSAVLKSGKCTADIGGTETTTSFTKAVIQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]