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The map label for this gene is cca [H]

Identifier: 218234591

GI number: 218234591

Start: 1517595

End: 1518788

Strand: Direct

Name: cca [H]

Synonym: BCB4264_A1592

Alternate gene names: 218234591

Gene position: 1517595-1518788 (Clockwise)

Preceding gene: 218235377

Following gene: 218233846

Centisome position: 28.0

GC content: 38.19

Gene sequence:

>1194_bases
ATGGAAAGATTTAAAAAAGCTAGTTCTATTATTGAGACATTAAAGCAACAAGGACATGAGGCTTACTTTGTTGGTGGGAG
CGTACGAGATCTTATTATCGATAGGCCGATTGGAGATATTGATATTGCGACATCTGCTCTACCAGAAGAAGTGATGGCTA
TATTCCCAAGAAATGTTCCTGTTGGTCTTGAGCATGGAACTGTAATTGTTGTAGAAAATGGTGAGCCCTATGAGGTAACT
ACTTTTCGAACAGAAAGCGAATATGAAGATTTTCGAAGACCTAGTAGTGTTCAATTTGTTCGTTCATTAGAAGAGGATTT
AAAACGTCGTGATTTCACGATGAATGCAATTGCTATGACAGAAGAAGGCAAAATGGTCGATTTATTTGCTGGACAGGAAG
CCATTCAACAGAGAGAAATTGTGACAGTTGGAAATGCTGCGGATCGTTTTCAAGAAGATGCCCTGCGAATGATGCGTGGT
ATTCGGTTTGTAAGCACGTTAGGTTTTTCATTAGAAATGAAAACGAAGCAAGCGATTGAAACGTATGGGCATTTGCTAGA
ACATATAGCAATTGAGCGTATTACAGTAGAGTTTGAGAAACTGTTAACTGGTACATATTGTGTGAAAGGTCTCAAAGAAT
TAGTAGAGACGAAGCTATTTTCTCATTTGCCATATTTACAAATGTCAGAAGAGAGACTGTTAAAAGCTACGCAGTATAAA
TGGGATTCTTTCGAAACGGATATTGAGGCGTGGGCATTTTTCTTATATTGTATCGGAGAAGAACATCCATCTGTCTTTTT
ACGTCAATGGAAGTTTTCGAATAAAAAAATAAAAGATATTGTTGCAGTTTTATTGACAATCCGTACTAGAAAGGAAAAGG
ATTGGGATACAGTCCTTCTTTATAAAACGGGAATTCATATCGCTGAAATGGCAGAGAGAGTATATGAAGCGATGATTGAA
CGCTATGACCCTACATCTGTTGAACGAGTACAATCAATGTTTCATGCATTGCCAATCCAGGAGCGTCAAGAAATGAATGT
GACTGGTAATGATTTATTAAACTGGGCAAACAAAAAGCCTGGTCCGTGGGTTGCTGAGATGCTTCAGAAAATCGAAGAAG
CAATCGTACAAGGAAACGTAGTTAATGAGAAAGAGCGTATAAGGGAGTGGCTACAAGGATGCAATCTACTATAA

Upstream 100 bases:

>100_bases
GGAGAACGAGCAAGAGAAAGTGTTTATGAGCAATTTCGCTCAGAAAAGATTGTTTCACAATATGAAACGATTTACTATGA
CGTACTAAGGGATGACAAAA

Downstream 100 bases:

>100_bases
GAAAGCAGTTATTGCAAGTTTTTTCTGAAGCGGATGGCGAGTTTGTATCCGGCCAAACAATTAGTGATAAACTTGGTTGT
TCAAGAACTGCTGTGTGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRNVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGKMVDLFAGQEAIQQREIVTVGNAADRFQEDALRMMRG
IRFVSTLGFSLEMKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYK
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRTRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
RYDPTSVERVQSMFHALPIQERQEMNVTGNDLLNWANKKPGPWVAEMLQKIEEAIVQGNVVNEKERIREWLQGCNLL

Sequences:

>Translated_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRNVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGKMVDLFAGQEAIQQREIVTVGNAADRFQEDALRMMRG
IRFVSTLGFSLEMKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYK
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRTRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
RYDPTSVERVQSMFHALPIQERQEMNVTGNDLLNWANKKPGPWVAEMLQKIEEAIVQGNVVNEKERIREWLQGCNLL
>Mature_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRNVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGKMVDLFAGQEAIQQREIVTVGNAADRFQEDALRMMRG
IRFVSTLGFSLEMKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYK
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRTRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
RYDPTSVERVQSMFHALPIQERQEMNVTGNDLLNWANKKPGPWVAEMLQKIEEAIVQGNVVNEKERIREWLQGCNLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=207, Percent_Identity=31.8840579710145, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=32.6086956521739, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789436, Length=190, Percent_Identity=32.1052631578947, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=268, Percent_Identity=29.4776119402985, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281360112, Length=269, Percent_Identity=29.7397769516729, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357337, Length=269, Percent_Identity=29.7397769516729, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46016; Mature: 46016

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRNVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
EEGKMVDLFAGQEAIQQREIVTVGNAADRFQEDALRMMRGIRFVSTLGFSLEMKTKQAIE
CCCCEEEEECCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRTRKEKDWDTVLL
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
YKTGIHIAEMAERVYEAMIERYDPTSVERVQSMFHALPIQERQEMNVTGNDLLNWANKKP
EECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCCC
GPWVAEMLQKIEEAIVQGNVVNEKERIREWLQGCNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRNVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
EEGKMVDLFAGQEAIQQREIVTVGNAADRFQEDALRMMRGIRFVSTLGFSLEMKTKQAIE
CCCCEEEEECCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRTRKEKDWDTVLL
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
YKTGIHIAEMAERVYEAMIERYDPTSVERVQSMFHALPIQERQEMNVTGNDLLNWANKKP
EECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCCC
GPWVAEMLQKIEEAIVQGNVVNEKERIREWLQGCNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA