Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 218233846

GI number: 218233846

Start: 1518773

End: 1519753

Strand: Direct

Name: birA [H]

Synonym: BCB4264_A1593

Alternate gene names: 218233846

Gene position: 1518773-1519753 (Clockwise)

Preceding gene: 218234591

Following gene: 218233138

Centisome position: 28.03

GC content: 39.35

Gene sequence:

>981_bases
ATGCAATCTACTATAAGAAAGCAGTTATTGCAAGTTTTTTCTGAAGCGGATGGCGAGTTTGTATCCGGCCAAACAATTAG
TGATAAACTTGGTTGTTCAAGAACTGCTGTGTGGAAACATATGGAGGATCTGCGGAGTGAGGGATATGAACTTGAAGCTG
TGCGTCGATTAGGTTACCGAATTGCAAGTAAGCCAGACAAAGTGACCGCTAATGAAATTCAGTTAGGGCTACAAACGAAC
CGGATTGGTAGAACAGTGTATTTTGAAGAAACTGTTGAATCAACGCAGCACATTGCAGCAAAACTTGCTTATGAAGGTGC
AGAAGAAGGAACTGTCGTTGTAGCAGAAGAACAAACAGCGGGAAGAGGTCGTTTAAGCAGAAAATGGCATTCACCAAAAG
GAACAGGGATTTGGATGAGTATTATTTTACGCCCATCAATCCCAGTTCATCATGCACCGCAACTTACTTTGTTAGCGGCC
GTTAGTGTTGCACAGGCAATTGAAAAATGCACGGGTGTAAACGTAGGAATAAAATGGCCAAATGATATTTTAATTCAAGG
TAAAAAGGCTGTAGGTATATTAACAGAAATGCAAGCGGATCCTGATAAAATTAATGCTGTCATTATGGGCATCGGTATTA
ATGCGAATCAAAAGCAAGAACATTTTGATGAAGAAATTCAGCACATTGCTACTTCATTAGCGATTGAATCAGGTAAGCCG
ATTGTTCGTGCAGAACTTATGCAACAAATCTATTTACAACTAGAAAAATTATATGAAGAGTATTTACAAAATGGTTTCTC
TGTCATTAAAATTCTTTGGGAAAGTTACGCTGTGAGCATCGGGAAAGAAATTACCGCTCGAACGATGAGAGAGACAATTA
CGGGGTTAGCAAAAGGAATCACAGAGGATGGTGTATTACTTCTGGAGGATCATGAGGGGAAAGTACATCATATTCATTCT
GCAGATATCGAAATTAAGTAA

Upstream 100 bases:

>100_bases
CTGGTCCGTGGGTTGCTGAGATGCTTCAGAAAATCGAAGAAGCAATCGTACAAGGAAACGTAGTTAATGAGAAAGAGCGT
ATAAGGGAGTGGCTACAAGG

Downstream 100 bases:

>100_bases
AAGAAGTTCCTCTTTTGGAACTTCTTTTTATTTCAATAGCTTTGAGTTGAATTTGTAACATAATAATAAATTTTTTTCAT
ATTTAAAGTTCATTTCAAAA

Product: biotin operon repressor/biotin-[acetyl-CoA-carboxylase] ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRSEGYELEAVRRLGYRIASKPDKVTANEIQLGLQTN
RIGRTVYFEETVESTQHIAAKLAYEGAEEGTVVVAEEQTAGRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKP
IVRAELMQQIYLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEITARTMRETITGLAKGITEDGVLLLEDHEGKVHHIHS
ADIEIK

Sequences:

>Translated_326_residues
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRSEGYELEAVRRLGYRIASKPDKVTANEIQLGLQTN
RIGRTVYFEETVESTQHIAAKLAYEGAEEGTVVVAEEQTAGRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKP
IVRAELMQQIYLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEITARTMRETITGLAKGITEDGVLLLEDHEGKVHHIHS
ADIEIK
>Mature_326_residues
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRSEGYELEAVRRLGYRIASKPDKVTANEIQLGLQTN
RIGRTVYFEETVESTQHIAAKLAYEGAEEGTVVVAEEQTAGRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKP
IVRAELMQQIYLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEITARTMRETITGLAKGITEDGVLLLEDHEGKVHHIHS
ADIEIK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=321, Percent_Identity=25.8566978193146, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1790408, Length=312, Percent_Identity=28.2051282051282, Blast_Score=120, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24644246, Length=237, Percent_Identity=28.6919831223629, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36230; Mature: 36230

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRSEGYELEAVRRLGYR
CCHHHHHHHHHHHHHCCCCEECCCCCHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
IASKPDKVTANEIQLGLQTNRIGRTVYFEETVESTQHIAAKLAYEGAEEGTVVVAEEQTA
HCCCCCCCCHHHEEECEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAAVSVAQAIEKCTGVNVGIKWP
CCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
NDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKP
CCEEEECCHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IVRAELMQQIYLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEITARTMRETITGLAKGI
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TEDGVLLLEDHEGKVHHIHSADIEIK
CCCCEEEEECCCCCEEEEECCCCEEC
>Mature Secondary Structure
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRSEGYELEAVRRLGYR
CCHHHHHHHHHHHHHCCCCEECCCCCHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
IASKPDKVTANEIQLGLQTNRIGRTVYFEETVESTQHIAAKLAYEGAEEGTVVVAEEQTA
HCCCCCCCCHHHEEECEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAAVSVAQAIEKCTGVNVGIKWP
CCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
NDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKP
CCEEEECCHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IVRAELMQQIYLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEITARTMRETITGLAKGI
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TEDGVLLLEDHEGKVHHIHSADIEIK
CCCCEEEEECCCCCEEEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]