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The map label for this gene is vanS [H]

Identifier: 218234352

GI number: 218234352

Start: 3049779

End: 3051239

Strand: Reverse

Name: vanS [H]

Synonym: BCB4264_A3168

Alternate gene names: 218234352

Gene position: 3051239-3049779 (Counterclockwise)

Preceding gene: 218233819

Following gene: 218234841

Centisome position: 56.31

GC content: 29.16

Gene sequence:

>1461_bases
ATGATCGATCGTACGAAATTAAGGGATAATATATTCTTTTCTACACGCTTAAATATAAAGCTTATTTTAATTTTTTTGCT
CTGTTTCATCGTTGCGGTTGTTACTTTTTTTCTCATAGCAATGAAAAGTGCAAATCTACTTTCTTATAATCAACAGATTG
AAAAAGAAGCCGAAATAAGGGAAATAGCGATAGGGATAACTAATGATATACAAAAGCGTAATTTGGAATCTGGGCATATA
GAACAAGTTATACCGATGATTCAATCTATCCAAAAGAAGAATGAAAATTTAAGGTTTTTAGTATTAGATTATAAAGGCCA
TATTATCTATCGTTCACATAATTTTCAATTGCAGGATAATCTCCCACAGGAATTATTACAGGAGAAATTAAATGTTACAC
CAAACGTCAGAAATCCTTTGTTTGAGAAGTTCATATTGTTTGTTTCACCAGTTTCATTTCAAGATGGAATTGGATATGTA
ATGGTACAATCTCCAAATTCGAATAAAACAATCTCACCAACTCAATATAATCAAAATTTATTACTTGCTTTGGTGAGCAG
TATATTAATTTTCATCCTTTTATTTTTGCTGTTTATGATCCCTATTACAAGATACATTAAAGAAATTGAACGAGGTATTC
AAAAGATTGTAAAAAAAGATTGGAAACATTCCATACGTGTTAAAGGAAAAGATGAATTATCTTCTTTAGCTTATAATATT
AATTGGATGGCTAGTCAATTAAGGGAGCGTTTTGAGAAAGAGAGAGCAATTGAGAAAGGTAAAAGTGAATTTATTACCAA
TATTTCTCATGATTTGCGTACCCCTTTAACGTCTATTATTGGATATTTAGCATTAGTAAATGAAAAACAATATCGAAATA
AAGAAGAATTAGAAATGTATATTCAAAATACGTATAATTTAACATTAAAATTAAAAACTCTCATTAATGAATTGTTTGAA
TACACAAAATTATCTTTACCTGATGTTAAGTTGAGAAAAGAAAAAGTAGAATTGGGCAATTTGTTAGGTCAGCTAGTAGG
AGAGTATATCCCGATTTTTGAAAAACAGCAATTAATTGTTAAACTAAATATCATTAATAAGGATATTTGGGTATGGGCAG
ATATAGAAAAACTGGTGCGTGTATTTGATAATTTACTATCTAATACTGAAAAATATAGTTTTTCAAATTCAACAGTAATA
GTGGAACTAAAAAAAGAAGAAGATAAGGCATTTATTTGTATTACAAATAAAACAGATCATCTTGAAGCACAAGAATTAAG
CAAGATGTTTGAGAAGTTTTATCGAGTTGATAAATCACGGTCGAGTCAAATACACGGATCCGGAATTGGTTTAGCAATTG
TAAAGCGCACAATTGAATTGCATGAGGGCAAAGTATGGGCTGAAAGTCATGGAAATAGTCTTACGATACATGTAGAATTA
CAACTTTTAAAAAATGTTTAA

Upstream 100 bases:

>100_bases
ATACAGTCATGGTTCATATTACTAAAATACGGGAAAAAATTGAAGAAAACCCTCGTCAGCCTGTTTATTTAAAAACAGTA
TGGGGAGTTGGCTATAGAGT

Downstream 100 bases:

>100_bases
AAACTATTTAAGAATTTTCATTATGTAACTAATATAAATGCTTAAATAAAGCATTTATAAACAATAGTAGGCTTCTTTTT
GTAACCATTGCATGAATGCC

Product: sensory transduction protein kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MIDRTKLRDNIFFSTRLNIKLILIFLLCFIVAVVTFFLIAMKSANLLSYNQQIEKEAEIREIAIGITNDIQKRNLESGHI
EQVIPMIQSIQKKNENLRFLVLDYKGHIIYRSHNFQLQDNLPQELLQEKLNVTPNVRNPLFEKFILFVSPVSFQDGIGYV
MVQSPNSNKTISPTQYNQNLLLALVSSILIFILLFLLFMIPITRYIKEIERGIQKIVKKDWKHSIRVKGKDELSSLAYNI
NWMASQLRERFEKERAIEKGKSEFITNISHDLRTPLTSIIGYLALVNEKQYRNKEELEMYIQNTYNLTLKLKTLINELFE
YTKLSLPDVKLRKEKVELGNLLGQLVGEYIPIFEKQQLIVKLNIINKDIWVWADIEKLVRVFDNLLSNTEKYSFSNSTVI
VELKKEEDKAFICITNKTDHLEAQELSKMFEKFYRVDKSRSSQIHGSGIGLAIVKRTIELHEGKVWAESHGNSLTIHVEL
QLLKNV

Sequences:

>Translated_486_residues
MIDRTKLRDNIFFSTRLNIKLILIFLLCFIVAVVTFFLIAMKSANLLSYNQQIEKEAEIREIAIGITNDIQKRNLESGHI
EQVIPMIQSIQKKNENLRFLVLDYKGHIIYRSHNFQLQDNLPQELLQEKLNVTPNVRNPLFEKFILFVSPVSFQDGIGYV
MVQSPNSNKTISPTQYNQNLLLALVSSILIFILLFLLFMIPITRYIKEIERGIQKIVKKDWKHSIRVKGKDELSSLAYNI
NWMASQLRERFEKERAIEKGKSEFITNISHDLRTPLTSIIGYLALVNEKQYRNKEELEMYIQNTYNLTLKLKTLINELFE
YTKLSLPDVKLRKEKVELGNLLGQLVGEYIPIFEKQQLIVKLNIINKDIWVWADIEKLVRVFDNLLSNTEKYSFSNSTVI
VELKKEEDKAFICITNKTDHLEAQELSKMFEKFYRVDKSRSSQIHGSGIGLAIVKRTIELHEGKVWAESHGNSLTIHVEL
QLLKNV
>Mature_486_residues
MIDRTKLRDNIFFSTRLNIKLILIFLLCFIVAVVTFFLIAMKSANLLSYNQQIEKEAEIREIAIGITNDIQKRNLESGHI
EQVIPMIQSIQKKNENLRFLVLDYKGHIIYRSHNFQLQDNLPQELLQEKLNVTPNVRNPLFEKFILFVSPVSFQDGIGYV
MVQSPNSNKTISPTQYNQNLLLALVSSILIFILLFLLFMIPITRYIKEIERGIQKIVKKDWKHSIRVKGKDELSSLAYNI
NWMASQLRERFEKERAIEKGKSEFITNISHDLRTPLTSIIGYLALVNEKQYRNKEELEMYIQNTYNLTLKLKTLINELFE
YTKLSLPDVKLRKEKVELGNLLGQLVGEYIPIFEKQQLIVKLNIINKDIWVWADIEKLVRVFDNLLSNTEKYSFSNSTVI
VELKKEEDKAFICITNKTDHLEAQELSKMFEKFYRVDKSRSSQIHGSGIGLAIVKRTIELHEGKVWAESHGNSLTIHVEL
QLLKNV

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=282, Percent_Identity=26.9503546099291, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=26.6891891891892, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786600, Length=213, Percent_Identity=28.169014084507, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=25.4098360655738, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082128, Length=317, Percent_Identity=25.5520504731861, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788713, Length=295, Percent_Identity=24.406779661017, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=285, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787894, Length=274, Percent_Identity=24.0875912408759, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=26.0330578512397, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=25.1082251082251, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=207, Percent_Identity=25.1207729468599, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789808, Length=305, Percent_Identity=21.9672131147541, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790551, Length=285, Percent_Identity=22.4561403508772, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788279, Length=302, Percent_Identity=25.4966887417219, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56679; Mature: 56679

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRTKLRDNIFFSTRLNIKLILIFLLCFIVAVVTFFLIAMKSANLLSYNQQIEKEAEIR
CCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EIAIGITNDIQKRNLESGHIEQVIPMIQSIQKKNENLRFLVLDYKGHIIYRSHNFQLQDN
HHEECCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCEECCC
LPQELLQEKLNVTPNVRNPLFEKFILFVSPVSFQDGIGYVMVQSPNSNKTISPTQYNQNL
CHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHH
LLALVSSILIFILLFLLFMIPITRYIKEIERGIQKIVKKDWKHSIRVKGKDELSSLAYNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
NWMASQLRERFEKERAIEKGKSEFITNISHDLRTPLTSIIGYLALVNEKQYRNKEELEMY
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
IQNTYNLTLKLKTLINELFEYTKLSLPDVKLRKEKVELGNLLGQLVGEYIPIFEKQQLIV
HHCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KLNIINKDIWVWADIEKLVRVFDNLLSNTEKYSFSNSTVIVELKKEEDKAFICITNKTDH
EEEEECCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCC
LEAQELSKMFEKFYRVDKSRSSQIHGSGIGLAIVKRTIELHEGKVWAESHGNSLTIHVEL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEEE
QLLKNV
EEHHCC
>Mature Secondary Structure
MIDRTKLRDNIFFSTRLNIKLILIFLLCFIVAVVTFFLIAMKSANLLSYNQQIEKEAEIR
CCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EIAIGITNDIQKRNLESGHIEQVIPMIQSIQKKNENLRFLVLDYKGHIIYRSHNFQLQDN
HHEECCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCEECCC
LPQELLQEKLNVTPNVRNPLFEKFILFVSPVSFQDGIGYVMVQSPNSNKTISPTQYNQNL
CHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHH
LLALVSSILIFILLFLLFMIPITRYIKEIERGIQKIVKKDWKHSIRVKGKDELSSLAYNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
NWMASQLRERFEKERAIEKGKSEFITNISHDLRTPLTSIIGYLALVNEKQYRNKEELEMY
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
IQNTYNLTLKLKTLINELFEYTKLSLPDVKLRKEKVELGNLLGQLVGEYIPIFEKQQLIV
HHCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KLNIINKDIWVWADIEKLVRVFDNLLSNTEKYSFSNSTVIVELKKEEDKAFICITNKTDH
EEEEECCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCC
LEAQELSKMFEKFYRVDKSRSSQIHGSGIGLAIVKRTIELHEGKVWAESHGNSLTIHVEL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEEE
QLLKNV
EEHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]