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The map label for this gene is lytF [H]

Identifier: 218234140

GI number: 218234140

Start: 996623

End: 997963

Strand: Direct

Name: lytF [H]

Synonym: BCB4264_A1016

Alternate gene names: 218234140

Gene position: 996623-997963 (Clockwise)

Preceding gene: 218234701

Following gene: 218235119

Centisome position: 18.39

GC content: 36.91

Gene sequence:

>1341_bases
ATGTTTAAGAAAAGTATAGGAATCTTATTGTTTTTTATTCTATCTATCTCGACATTCAGTATGGTAACTCACGCTGCTAA
TAGTTCAGAATACGTGAATCAATCATTCTATGGGTATAAAGAACCATCTTTTAATTCAGCTAAAACAAATGGCGGATCAG
AGTATGGTGCTCAAAATGTAGGAGTCATGGAAAAAAGAGATAATGGCTGGTGGAAGATTGAAACATGGGAAGGTCCAGTT
TGGATTAACTTGAATGGGGAAGAACGTGTCATGGGAGATTTCTATGCATATGATGAACCTTCTCTCTCATCAAAGGTTGC
GAATGCTGGAGCGAAGTACGGGCGACAAACATTTAGAATAGTAGATGGAACAACAGATGGTTGGCTTAAATTTAAGACGT
GGGAAGGCGAAAAATGGATGAATCCTGCGGCTGAACAAATTACCGTCAACAAAACGATTTATGCATATAATGAGCCTTCA
TTTAATGCACAGAAAGCAAATTATGGAGCACCATTTAACCCACAAAATTGGGGAGTAGTAGAAAAAAAAGAAAATGGCTG
GATGAAGGTAAGTACTTATGAAGGTTATAAGTGGATCAATCCAGATGGAGAAGAAAGATTCATAAACAAAGCATTCTATG
CGTATAACGAAGCTTCATTTAATGCAGGAAAAGCAAACGCTGGAGCATTATATAATCCGCAGAATTTTAGAGTTGTGGAT
GGGACACCTAATGGATGGTTAAAGGTTAAAACATGGGAAGGCGAGAAATGGCTGAATCTAGATGGAGAAGAAAGGTTCAT
AGAAAAAGCATTCTATGCGTATAACGAGCCTTCATTTAGTTCAGGCAAAGCAAACGCTGGAGCATTATATAGTCCGCAGA
ATTTTAGAGTAATAGATGGGACAACTAGTGGATGGTTGAAGATTAAAACATGGGAAGGCGACAAGTGGATGAACCTAAAC
CAAACAGATTCTGGTTCTTCTGGAGTAATTGATTTAGCTTTAAAACAACTTGGAAAGCCGTATGTTGAAGCTGGGACAGG
ACCAAATGGATTTGATTGTAGTGGCTTTATTTATTACGTATTTAAAAATAATGGATATAACATTGTGAGAACAAGTGTTG
CAGGATACTGGGGAATGGTTACAAAAATAAGTGATCCTCAACCAGGAGATTTAGTATTCTTACAGAATACATATAAAGCG
GGTCCTTCTCATTTAGGAATATATTTAGGGAATGGTGAATATATTCATGCTGCTGACGAAACAACAGGTGTAATTAAAAG
TAAGATAAATAGTTCATACACTCAAAAGCACTTCTTGGGGTATGCTAGATTTTCAAAATAA

Upstream 100 bases:

>100_bases
GGGGACGTAAAAAAGTGAGAATTAAAATAATGAAATAGGATATATCTATTCTTTGCTTCGAAAATTATATATTAGACAAA
ATAAAAGGGAGGATATTTTT

Downstream 100 bases:

>100_bases
TTAAATTGGATTCAATAAAAAGACAGCTATCTATGTGAACTGCACCCCAATTGTTAGACACAGTCTAACAATTGGAGGTG
TAGTTTTTTTTATGACTAAA

Product: NlpC/P60 family protein

Products: NA

Alternate protein names: Cell wall-associated polypeptide CWBP49; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytF [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MFKKSIGILLFFILSISTFSMVTHAANSSEYVNQSFYGYKEPSFNSAKTNGGSEYGAQNVGVMEKRDNGWWKIETWEGPV
WINLNGEERVMGDFYAYDEPSLSSKVANAGAKYGRQTFRIVDGTTDGWLKFKTWEGEKWMNPAAEQITVNKTIYAYNEPS
FNAQKANYGAPFNPQNWGVVEKKENGWMKVSTYEGYKWINPDGEERFINKAFYAYNEASFNAGKANAGALYNPQNFRVVD
GTPNGWLKVKTWEGEKWLNLDGEERFIEKAFYAYNEPSFSSGKANAGALYSPQNFRVIDGTTSGWLKIKTWEGDKWMNLN
QTDSGSSGVIDLALKQLGKPYVEAGTGPNGFDCSGFIYYVFKNNGYNIVRTSVAGYWGMVTKISDPQPGDLVFLQNTYKA
GPSHLGIYLGNGEYIHAADETTGVIKSKINSSYTQKHFLGYARFSK

Sequences:

>Translated_446_residues
MFKKSIGILLFFILSISTFSMVTHAANSSEYVNQSFYGYKEPSFNSAKTNGGSEYGAQNVGVMEKRDNGWWKIETWEGPV
WINLNGEERVMGDFYAYDEPSLSSKVANAGAKYGRQTFRIVDGTTDGWLKFKTWEGEKWMNPAAEQITVNKTIYAYNEPS
FNAQKANYGAPFNPQNWGVVEKKENGWMKVSTYEGYKWINPDGEERFINKAFYAYNEASFNAGKANAGALYNPQNFRVVD
GTPNGWLKVKTWEGEKWLNLDGEERFIEKAFYAYNEPSFSSGKANAGALYSPQNFRVIDGTTSGWLKIKTWEGDKWMNLN
QTDSGSSGVIDLALKQLGKPYVEAGTGPNGFDCSGFIYYVFKNNGYNIVRTSVAGYWGMVTKISDPQPGDLVFLQNTYKA
GPSHLGIYLGNGEYIHAADETTGVIKSKINSSYTQKHFLGYARFSK
>Mature_446_residues
MFKKSIGILLFFILSISTFSMVTHAANSSEYVNQSFYGYKEPSFNSAKTNGGSEYGAQNVGVMEKRDNGWWKIETWEGPV
WINLNGEERVMGDFYAYDEPSLSSKVANAGAKYGRQTFRIVDGTTDGWLKFKTWEGEKWMNPAAEQITVNKTIYAYNEPS
FNAQKANYGAPFNPQNWGVVEKKENGWMKVSTYEGYKWINPDGEERFINKAFYAYNEASFNAGKANAGALYNPQNFRVVD
GTPNGWLKVKTWEGEKWLNLDGEERFIEKAFYAYNEPSFSSGKANAGALYSPQNFRVIDGTTSGWLKIKTWEGDKWMNLN
QTDSGSSGVIDLALKQLGKPYVEAGTGPNGFDCSGFIYYVFKNNGYNIVRTSVAGYWGMVTKISDPQPGDLVFLQNTYKA
GPSHLGIYLGNGEYIHAADETTGVIKSKINSSYTQKHFLGYARFSK

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=138, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787944, Length=120, Percent_Identity=30, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 50090; Mature: 50090

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKSIGILLFFILSISTFSMVTHAANSSEYVNQSFYGYKEPSFNSAKTNGGSEYGAQNV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
GVMEKRDNGWWKIETWEGPVWINLNGEERVMGDFYAYDEPSLSSKVANAGAKYGRQTFRI
CEEEECCCCCEEEEEECCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEE
VDGTTDGWLKFKTWEGEKWMNPAAEQITVNKTIYAYNEPSFNAQKANYGAPFNPQNWGVV
EECCCCCEEEEEEECCCHHCCCCHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
EKKENGWMKVSTYEGYKWINPDGEERFINKAFYAYNEASFNAGKANAGALYNPQNFRVVD
EECCCCCEEEEEECCEEEECCCCHHHHHHHHHEEECCCCCCCCCCCCCEEECCCCCEEEE
GTPNGWLKVKTWEGEKWLNLDGEERFIEKAFYAYNEPSFSSGKANAGALYSPQNFRVIDG
CCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEEC
TTSGWLKIKTWEGDKWMNLNQTDSGSSGVIDLALKQLGKPYVEAGTGPNGFDCSGFIYYV
CCCCEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
FKNNGYNIVRTSVAGYWGMVTKISDPQPGDLVFLQNTYKAGPSHLGIYLGNGEYIHAADE
EECCCCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEECCC
TTGVIKSKINSSYTQKHFLGYARFSK
CCHHHHHHHCCHHHHHHHEEEHCCCC
>Mature Secondary Structure
MFKKSIGILLFFILSISTFSMVTHAANSSEYVNQSFYGYKEPSFNSAKTNGGSEYGAQNV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
GVMEKRDNGWWKIETWEGPVWINLNGEERVMGDFYAYDEPSLSSKVANAGAKYGRQTFRI
CEEEECCCCCEEEEEECCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEE
VDGTTDGWLKFKTWEGEKWMNPAAEQITVNKTIYAYNEPSFNAQKANYGAPFNPQNWGVV
EECCCCCEEEEEEECCCHHCCCCHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
EKKENGWMKVSTYEGYKWINPDGEERFINKAFYAYNEASFNAGKANAGALYNPQNFRVVD
EECCCCCEEEEEECCEEEECCCCHHHHHHHHHEEECCCCCCCCCCCCCEEECCCCCEEEE
GTPNGWLKVKTWEGEKWLNLDGEERFIEKAFYAYNEPSFSSGKANAGALYSPQNFRVIDG
CCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEEC
TTSGWLKIKTWEGDKWMNLNQTDSGSSGVIDLALKQLGKPYVEAGTGPNGFDCSGFIYYV
CCCCEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
FKNNGYNIVRTSVAGYWGMVTKISDPQPGDLVFLQNTYKAGPSHLGIYLGNGEYIHAADE
EECCCCEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEECCC
TTGVIKSKINSSYTQKHFLGYARFSK
CCHHHHHHHCCHHHHHHHEEEHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 10206711 [H]