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The map label for this gene is kinD [H]

Identifier: 218233885

GI number: 218233885

Start: 1344364

End: 1345860

Strand: Reverse

Name: kinD [H]

Synonym: BCB4264_A1387

Alternate gene names: 218233885

Gene position: 1345860-1344364 (Counterclockwise)

Preceding gene: 218233859

Following gene: 218235875

Centisome position: 24.84

GC content: 33.67

Gene sequence:

>1497_bases
TTGAAAGTCAAATTCCAAAGCATAATTGTATATATATTGCTTGTTATCCTACCGACAATAGGGATTGGTGCTACATTTTA
TTCGTATCATACATATAAAATGAAACAGGAAAACAAATTATCTGCTCATACAGTTCTCTTTTTATATAGAGATTATTTGG
ATCATCATCTTGGTGAAGCAATTTCTGCTTTAGACATGCTTGCAAAAGTTGTAGGCACCGAGACTGGTAATATAAATGGC
ATTAAACAAATTGTACATGATACAGATGGAAACGATGCACGCTTTTCTGGTCTATATTACGCTACACCAGAAGGAGTCAT
TACAATCGCATCTGAAGATGAATCAAAGCCTATAGATGTTTCAGACCGTAAATATATTCAAGACGCGCTCCAAACTAAAA
AAACCACTGTATCATCAGTCATTACTGATCGCGTTCTTGGACATAAAGCTATTATGATTGCTGCCCCCATATTTAATAAA
CAGAAGGAGCTCTCAGGATTATTATTAGCTAGTTTACGCTTTGATTACATTTCATCTTCTTTAAATGCCATTAAACCACA
ATACCATTTTGAGGTAACTGATAAACATGATGTAGTTTTTCTAACCGATGATAATAACGACACAAGTGATACGCATTCTA
ATATGCTTACTACCCCACTCCAAAGATTAAATTGGAAGGTCTCTGTTTCTCCATTACCTATTCATCAAAAAACCTTATAC
CAGTGGGTTGCAATAGAGTGTATAGCTACTTTATTTTTAATGTCTATTTTATTTTTACTTGCTCAATATATGTTGTTAAA
ACGTCAAACAAAACTTGAAAGGCAACAAAATGAACTACAAAAAATTGAATTAGTTGGAACTTTTGCAGCGAGTACAGCAC
ATGAAATCCGTAATCCTCTTACTGGAATTAAAGGACTCGTCGCTCTATTAAAAGAGAAATATAAAGATGAGCAAGATCAG
TTCTACTTTTCCGTAATTGAACAAGAAATAGAGCGAATCAATGAAATTGTAAGTGAATTTCTTATTCTCGGAAAGCCAAC
CGCTATCATCGAGCAAACGTATGATGTAAGAAAAATTCTAAATGAGGTAGCATTAATTATTCAATCTGAGGCGAATTTAC
ATAACATTGTATTCTATTTACATTTGCCAGACCATCCTGTTCATATCCGTTGTTCGAAAGACCATATGAAACAAGTGGTT
TTAAACATTACAAAAAATGCAATTGAGGCCATGACTTCTAGTGATACACTAACCATTGTAGTAAAAAACAATGAAACACA
CGCACAATTGCAGATTATAGATACTGGAAAAGGGATTCCGCAACATATTCAAAAACACCTCTTTCATCCGTTCTTTACTA
ATAAAGATACTGGAACAGGTCTTGGACTTGTCATATGTAAACGAATTGTAGAGATGTATAATGGGCATATTTTTATTGAC
AGTACAGAAAACGAAGGAACAACAGTTCATATCGAAATTCCGTTACACATAGTATAA

Upstream 100 bases:

>100_bases
TCCTCTCCCCTAATATATTAAGAATCATTCTTGAATAAAAACATGACAATTTTAACATAGTTTATATATAATGATAATAA
AAAAAGAAGGAGTACCACAT

Downstream 100 bases:

>100_bases
TTATCCTTATCCTATAAGGGTAATTATACTTATTTCATAAAACCAGCGACAAGCATTCGCCAAAAATTATTTGATTTTCC
TTCATACCCGATTTGGAAAA

Product: sensor histidine kinase KinD

Products: NA

Alternate protein names: Sensor histidine kinase D [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MKVKFQSIIVYILLVILPTIGIGATFYSYHTYKMKQENKLSAHTVLFLYRDYLDHHLGEAISALDMLAKVVGTETGNING
IKQIVHDTDGNDARFSGLYYATPEGVITIASEDESKPIDVSDRKYIQDALQTKKTTVSSVITDRVLGHKAIMIAAPIFNK
QKELSGLLLASLRFDYISSSLNAIKPQYHFEVTDKHDVVFLTDDNNDTSDTHSNMLTTPLQRLNWKVSVSPLPIHQKTLY
QWVAIECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPLTGIKGLVALLKEKYKDEQDQ
FYFSVIEQEIERINEIVSEFLILGKPTAIIEQTYDVRKILNEVALIIQSEANLHNIVFYLHLPDHPVHIRCSKDHMKQVV
LNITKNAIEAMTSSDTLTIVVKNNETHAQLQIIDTGKGIPQHIQKHLFHPFFTNKDTGTGLGLVICKRIVEMYNGHIFID
STENEGTTVHIEIPLHIV

Sequences:

>Translated_498_residues
MKVKFQSIIVYILLVILPTIGIGATFYSYHTYKMKQENKLSAHTVLFLYRDYLDHHLGEAISALDMLAKVVGTETGNING
IKQIVHDTDGNDARFSGLYYATPEGVITIASEDESKPIDVSDRKYIQDALQTKKTTVSSVITDRVLGHKAIMIAAPIFNK
QKELSGLLLASLRFDYISSSLNAIKPQYHFEVTDKHDVVFLTDDNNDTSDTHSNMLTTPLQRLNWKVSVSPLPIHQKTLY
QWVAIECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPLTGIKGLVALLKEKYKDEQDQ
FYFSVIEQEIERINEIVSEFLILGKPTAIIEQTYDVRKILNEVALIIQSEANLHNIVFYLHLPDHPVHIRCSKDHMKQVV
LNITKNAIEAMTSSDTLTIVVKNNETHAQLQIIDTGKGIPQHIQKHLFHPFFTNKDTGTGLGLVICKRIVEMYNGHIFID
STENEGTTVHIEIPLHIV
>Mature_498_residues
MKVKFQSIIVYILLVILPTIGIGATFYSYHTYKMKQENKLSAHTVLFLYRDYLDHHLGEAISALDMLAKVVGTETGNING
IKQIVHDTDGNDARFSGLYYATPEGVITIASEDESKPIDVSDRKYIQDALQTKKTTVSSVITDRVLGHKAIMIAAPIFNK
QKELSGLLLASLRFDYISSSLNAIKPQYHFEVTDKHDVVFLTDDNNDTSDTHSNMLTTPLQRLNWKVSVSPLPIHQKTLY
QWVAIECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPLTGIKGLVALLKEKYKDEQDQ
FYFSVIEQEIERINEIVSEFLILGKPTAIIEQTYDVRKILNEVALIIQSEANLHNIVFYLHLPDHPVHIRCSKDHMKQVV
LNITKNAIEAMTSSDTLTIVVKNNETHAQLQIIDTGKGIPQHIQKHLFHPFFTNKDTGTGLGLVICKRIVEMYNGHIFID
STENEGTTVHIEIPLHIV

Specific function: Phosphorylates the sporulation-regulatory protein spo0F and, to a minor extent, is responsible for heterogeneous expression of spo0A during logarithmical growth. Also phosphorylates spo0A under biofilm growth conditions [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=31.7460317460317, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=30.1724137931034, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=27.6785714285714, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790300, Length=233, Percent_Identity=27.0386266094421, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789808, Length=213, Percent_Identity=27.2300469483568, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=27.8761061946903, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790861, Length=246, Percent_Identity=23.1707317073171, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081816, Length=240, Percent_Identity=23.3333333333333, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=249, Percent_Identity=22.4899598393574, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=24.2152466367713, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=24.6861924686192, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788713, Length=243, Percent_Identity=25.1028806584362, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=219, Percent_Identity=22.3744292237443, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56474; Mature: 56474

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKFQSIIVYILLVILPTIGIGATFYSYHTYKMKQENKLSAHTVLFLYRDYLDHHLGEA
CCCHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ISALDMLAKVVGTETGNINGIKQIVHDTDGNDARFSGLYYATPEGVITIASEDESKPIDV
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEECCEEEECCCCEEEEECCCCCCCCCC
SDRKYIQDALQTKKTTVSSVITDRVLGHKAIMIAAPIFNKQKELSGLLLASLRFDYISSS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LNAIKPQYHFEVTDKHDVVFLTDDNNDTSDTHSNMLTTPLQRLNWKVSVSPLPIHQKTLY
HHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
QWVAIECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIKGLVALLKEKYKDEQDQFYFSVIEQEIERINEIVSEFLILGKPTAIIEQTYDVRKIL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NEVALIIQSEANLHNIVFYLHLPDHPVHIRCSKDHMKQVVLNITKNAIEAMTSSDTLTIV
HHHHHHHCCCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VKNNETHAQLQIIDTGKGIPQHIQKHLFHPFFTNKDTGTGLGLVICKRIVEMYNGHIFID
EECCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
STENEGTTVHIEIPLHIV
CCCCCCCEEEEEEEEEEC
>Mature Secondary Structure
MKVKFQSIIVYILLVILPTIGIGATFYSYHTYKMKQENKLSAHTVLFLYRDYLDHHLGEA
CCCHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ISALDMLAKVVGTETGNINGIKQIVHDTDGNDARFSGLYYATPEGVITIASEDESKPIDV
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEECCEEEECCCCEEEEECCCCCCCCCC
SDRKYIQDALQTKKTTVSSVITDRVLGHKAIMIAAPIFNKQKELSGLLLASLRFDYISSS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LNAIKPQYHFEVTDKHDVVFLTDDNNDTSDTHSNMLTTPLQRLNWKVSVSPLPIHQKTLY
HHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
QWVAIECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIKGLVALLKEKYKDEQDQFYFSVIEQEIERINEIVSEFLILGKPTAIIEQTYDVRKIL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NEVALIIQSEANLHNIVFYLHLPDHPVHIRCSKDHMKQVVLNITKNAIEAMTSSDTLTIV
HHHHHHHCCCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VKNNETHAQLQIIDTGKGIPQHIQKHLFHPFFTNKDTGTGLGLVICKRIVEMYNGHIFID
EECCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
STENEGTTVHIEIPLHIV
CCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]