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The map label for this gene is gudB [H]

Identifier: 218233867

GI number: 218233867

Start: 1479359

End: 1480645

Strand: Direct

Name: gudB [H]

Synonym: BCB4264_A1546

Alternate gene names: 218233867

Gene position: 1479359-1480645 (Clockwise)

Preceding gene: 218233838

Following gene: 218234508

Centisome position: 27.3

GC content: 38.0

Gene sequence:

>1287_bases
ATGGTAGCCGAAAAGGGAACTCAAACAAAAACACAACAACAAAGGGAACAACATTTTGAACTATTGAATTCAACACAAAT
TGTTATTAGTGAAGCGTTAGAAAAATTGGGTTATCCAAACGAAGTATATGAATTATTAAAAGAACCAATTCGTATGATGA
CAGTGAAAATACCAGTTCGTATGGATGACGGGACTGTTAAAATATTTACAGGATATCGTGCGCAACATAATGATGCTGTT
GGTCCAACGAAAGGTGGAATTCGCTTCCATCCGAATGTAACAGAAAATGAAGTGAAAGCACTTTCAATCTGGATGAGTTT
AAAATGTGGTATTGTTGATTTACCATATGGTGGAGGTAAGGGTGGAATTATCTGTGATCCACGTGAAATGTCATTCCGTG
AGTTAGAAAGATTAAGCCGCGGTTATGTACGAGCAATTAGCCAAATCGTAGGTCCGACGAAAGATATTCCAGCTCCAGAT
GTATTCACAAACTCTCAAATTATGGCGTGGATGATGGATGAGTATAGCCGTATTGATGAATTTAATTCACCAGGATTTAT
TACAGGTAAACCGCTTGTATTAGGTGGATCACATGGACGTGAAACAGCAACAGCGAAAGGTGTAACAATTTGTATTCGCG
AAGCTGCTAAAAAACGTGGCATTGATATTAAGGGTGCACGCGTTGTTGTTCAAGGATTCGGTAATGCTGGTAGCTTCTTA
GCTAAATTTATGCATGATGCAGGTGCGAAAGTTATTGCAATCTCAGATGCTTACGGCGCGTTACATGATCCAAACGGATT
AGATATTGATTATTTATTAGACCGTCGTGATAGCTTCGGTACAGTAACAAAGCTATTTAACAATACAATTTCAAACAAAG
AATTGTTAGAACTTGATTGCGATATTTTAGTACCAGCTGCAATTGAGAACCAAATTACAGAAGAAAATGCTAATGATATT
AAAGCAAAAATCGTTGTTGAAGCTGCAAATGGTCCAACAACATTAGAAGCGACTAAAATTTTAACAGATCGCGGAATTTT
GCTTGTTCCAGATGTGTTAGCAAGTGCTGGTGGTGTAACAGTATCTTACTTTGAGTGGGTACAAAACAACCAAGGTTACT
ACTGGACTGAAGAAGAAGTAGAACAACGTTTAGAAAAAGTAATGGTAAGATCATTTGATTCTATTTATGAAACAGCACAA
GTTCGTAAAGTGAACATGCGCTTAGCGGCGTACATGGTTGGTGTTCGTAAAATGGCTGAAGCAAGCCGCTTCAGAGGTTG
GGTATAA

Upstream 100 bases:

>100_bases
ATATATTTGTTAGCGTTTTCTATTGCATTCCAAATTTAAGGGTGTATACTAGGCAATGAAGGTTTACTAAACAAGTGAAA
TTACAGGGGGTTTACTTACT

Downstream 100 bases:

>100_bases
AATATTATATCTTGAGAAAACGTGATTTCCTTTCGAGGGAATCACGTTTTTTTGTTGAAGTATTAAATGAGGTGTTGTAT
ATGGAGAATGAAATGCTGGA

Product: glutamate dehydrogenase, NAD-specific

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVAEKGTQTKTQQQREQHFELLNSTQIVISEALEKLGYPNEVYELLKEPIRMMTVKIPVRMDDGTVKIFTGYRAQHNDAV
GPTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKGGIICDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPD
VFTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFL
AKFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTISNKELLELDCDILVPAAIENQITEENANDI
KAKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVEQRLEKVMVRSFDSIYETAQ
VRKVNMRLAAYMVGVRKMAEASRFRGWV

Sequences:

>Translated_428_residues
MVAEKGTQTKTQQQREQHFELLNSTQIVISEALEKLGYPNEVYELLKEPIRMMTVKIPVRMDDGTVKIFTGYRAQHNDAV
GPTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKGGIICDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPD
VFTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFL
AKFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTISNKELLELDCDILVPAAIENQITEENANDI
KAKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVEQRLEKVMVRSFDSIYETAQ
VRKVNMRLAAYMVGVRKMAEASRFRGWV
>Mature_428_residues
MVAEKGTQTKTQQQREQHFELLNSTQIVISEALEKLGYPNEVYELLKEPIRMMTVKIPVRMDDGTVKIFTGYRAQHNDAV
GPTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGKGGIICDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPD
VFTNSQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFL
AKFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTISNKELLELDCDILVPAAIENQITEENANDI
KAKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVEQRLEKVMVRSFDSIYETAQ
VRKVNMRLAAYMVGVRKMAEASRFRGWV

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=341, Percent_Identity=40.4692082111437, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=40.1759530791789, Blast_Score=265, Evalue=4e-71,
Organism=Escherichia coli, GI1788059, Length=406, Percent_Identity=30.7881773399015, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=336, Percent_Identity=41.0714285714286, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6319256, Length=401, Percent_Identity=29.1770573566085, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=390, Percent_Identity=28.2051282051282, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553475, Length=340, Percent_Identity=42.6470588235294, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=42.6470588235294, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24649283, Length=331, Percent_Identity=42.5981873111783, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=41.9354838709677, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 47493; Mature: 47493

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAEKGTQTKTQQQREQHFELLNSTQIVISEALEKLGYPNEVYELLKEPIRMMTVKIPVR
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEEE
MDDGTVKIFTGYRAQHNDAVGPTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGK
ECCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHEEEEEEEECCEEECCCCCCC
GGIICDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSRIDE
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFL
CCCCCEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
AKFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTISNKELLELDC
HHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECC
DILVPAAIENQITEENANDIKAKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVT
CEEECHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEHHHHHHCCCEEEECHHHHCCCCCE
VSYFEWVQNNQGYYWTEEEVEQRLEKVMVRSFDSIYETAQVRKVNMRLAAYMVGVRKMAE
EEEHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRFRGWV
HHHHCCCC
>Mature Secondary Structure
MVAEKGTQTKTQQQREQHFELLNSTQIVISEALEKLGYPNEVYELLKEPIRMMTVKIPVR
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEEE
MDDGTVKIFTGYRAQHNDAVGPTKGGIRFHPNVTENEVKALSIWMSLKCGIVDLPYGGGK
ECCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHEEEEEEEECCEEECCCCCCC
GGIICDPREMSFRELERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSRIDE
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FNSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGIDIKGARVVVQGFGNAGSFL
CCCCCEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
AKFMHDAGAKVIAISDAYGALHDPNGLDIDYLLDRRDSFGTVTKLFNNTISNKELLELDC
HHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECC
DILVPAAIENQITEENANDIKAKIVVEAANGPTTLEATKILTDRGILLVPDVLASAGGVT
CEEECHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEHHHHHHCCCEEEECHHHHCCCCCE
VSYFEWVQNNQGYYWTEEEVEQRLEKVMVRSFDSIYETAQVRKVNMRLAAYMVGVRKMAE
EEEHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRFRGWV
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]