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The map label for this gene is hisH [H]

Identifier: 218233723

GI number: 218233723

Start: 1403398

End: 1404027

Strand: Direct

Name: hisH [H]

Synonym: BCB4264_A1461

Alternate gene names: 218233723

Gene position: 1403398-1404027 (Clockwise)

Preceding gene: 218235800

Following gene: 218231891

Centisome position: 25.9

GC content: 37.78

Gene sequence:

>630_bases
TTGATTGCCATTATAGATTATGGAATGGGGAATATTCGCAGTGTAGAGCAAGCATTAAAATATATTGGCGCAGAGTACAT
TGTAACGAGTAATAAAAAAGAGATATTGAGAAGCGATGGCGTGATTTTACCGGGAGTAGGTGCATTTCCGAAAGCGATGG
ACGTATTAGAAGAAAGAGATTTAGTGTGTGTACTAAAAGAGGTTTGTGATATAGGAAAACCACTCCTCGGTATATGTTTG
GGCATGCAGCTTTTATTTGAACGAAGTGAGGAACTAAAAGATTGTAGCGGGTTAGGTTTACTACCAGGGGAAATTCGTAA
GTTAAAAGTTTCGTATAAAATTCCTCATATGGGATGGAATGAATTAAGGAAGGAAAGAGAATTTCCGCTTTGGAATGGAT
TAGTGGATGGTTCTTTCGTGTATTATGTTCACTCATATTACGCGGATTGTCCAGATGAAATTGTATGCGGAGTAAGTGAT
TATGGAATGCAAGTACCAGGGTTTGTAGCGAAAGGAAATGTATTTGGTGCACAGTTTCATCCAGAAAAAAGTGGTGAAAT
TGGCATGCAAATATTAAAAAATTTTCAAGGGGTGGTAGAAGCATGGAAATCTTCCCAGCTATCGATTTAA

Upstream 100 bases:

>100_bases
AATTGAAGCATTATTTAAAGCTTTTGGTAGAGCGCTTAGAGAAGCGGTCGAAAAAAATGCCAACATTACTGGTGTAAATT
CAACGAAAGGGATGTTGTAA

Downstream 100 bases:

>100_bases
AAGAAGGACGATGCGTTAGACTTTATCAAGGAGAGTTTAGTAAAGAAACGGTGATGAATGAAGATCCGGTAGCGCAGGCA
ATCATATTTGAAAAGCTTGG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIAIIDYGMGNIRSVEQALKYIGAEYIVTSNKKEILRSDGVILPGVGAFPKAMDVLEERDLVCVLKEVCDIGKPLLGICL
GMQLLFERSEELKDCSGLGLLPGEIRKLKVSYKIPHMGWNELRKEREFPLWNGLVDGSFVYYVHSYYADCPDEIVCGVSD
YGMQVPGFVAKGNVFGAQFHPEKSGEIGMQILKNFQGVVEAWKSSQLSI

Sequences:

>Translated_209_residues
MIAIIDYGMGNIRSVEQALKYIGAEYIVTSNKKEILRSDGVILPGVGAFPKAMDVLEERDLVCVLKEVCDIGKPLLGICL
GMQLLFERSEELKDCSGLGLLPGEIRKLKVSYKIPHMGWNELRKEREFPLWNGLVDGSFVYYVHSYYADCPDEIVCGVSD
YGMQVPGFVAKGNVFGAQFHPEKSGEIGMQILKNFQGVVEAWKSSQLSI
>Mature_209_residues
MIAIIDYGMGNIRSVEQALKYIGAEYIVTSNKKEILRSDGVILPGVGAFPKAMDVLEERDLVCVLKEVCDIGKPLLGICL
GMQLLFERSEELKDCSGLGLLPGEIRKLKVSYKIPHMGWNELRKEREFPLWNGLVDGSFVYYVHSYYADCPDEIVCGVSD
YGMQVPGFVAKGNVFGAQFHPEKSGEIGMQILKNFQGVVEAWKSSQLSI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=38.974358974359, Blast_Score=134, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=31.25, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23244; Mature: 23244

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGMGNIRSVEQALKYIGAEYIVTSNKKEILRSDGVILPGVGAFPKAMDVLEERD
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHCCCEEECCCCCCHHHHHHHHHCC
LVCVLKEVCDIGKPLLGICLGMQLLFERSEELKDCSGLGLLPGEIRKLKVSYKIPHMGWN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEECCCCCHH
ELRKEREFPLWNGLVDGSFVYYVHSYYADCPDEIVCGVSDYGMQVPGFVAKGNVFGAQFH
HHHHHCCCCHHCCCCCCHHHHHHHHHHCCCHHHHCCCCHHCCCCCCCEEECCCEECCCCC
PEKSGEIGMQILKNFQGVVEAWKSSQLSI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIAIIDYGMGNIRSVEQALKYIGAEYIVTSNKKEILRSDGVILPGVGAFPKAMDVLEERD
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHCCCEEECCCCCCHHHHHHHHHCC
LVCVLKEVCDIGKPLLGICLGMQLLFERSEELKDCSGLGLLPGEIRKLKVSYKIPHMGWN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEECCCCCHH
ELRKEREFPLWNGLVDGSFVYYVHSYYADCPDEIVCGVSDYGMQVPGFVAKGNVFGAQFH
HHHHHCCCCHHCCCCCCHHHHHHHHHHCCCHHHHCCCCHHCCCCCCCEEECCCEECCCCC
PEKSGEIGMQILKNFQGVVEAWKSSQLSI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA