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The map label for this gene is liaR [H]

Identifier: 218232967

GI number: 218232967

Start: 1431255

End: 1431887

Strand: Direct

Name: liaR [H]

Synonym: BCB4264_A1495

Alternate gene names: 218232967

Gene position: 1431255-1431887 (Clockwise)

Preceding gene: 218232183

Following gene: 218234380

Centisome position: 26.41

GC content: 37.28

Gene sequence:

>633_bases
ATGATAAAAGTATTGCTTGTCGATGATCATGAAATGGTTCGTATGGGTGTATCGGCATATTTATCGACACAGCCAGATAT
TGAAGTAGTAGGGGAAGCTGAGAATGGAAGAAAAGGATCAGAATTAGCATTGCAATTAAGGCCGGATATTATTTTAATGG
ACCTTGTTATGGATGAGATGGATGGTGTCGAAGCAACACGTGCTATTATTCAAGAATGGCCAGAAGCAAAAATTGTAGTT
GTAACAAGCTTTTTAGATGACGAAAAATTGTATCCTGTTATTGAGGCTGGGGCGACCAGTTACTTATTAAAAACATCAAG
AGCAAGTGATATTGCTGATGCTGTACGAGCTACTTATGATGGAGAAACAGTATTAGAGCCGAAAGTTACCGGGAAAATGA
TGTCCCGTATGCGTCAAAAGAAAGAGCAACCTTTGCATGATGATTTAACGGAAAGAGAGTTTGAGATTTTGTTATTAATT
GCAGAGGGAAAAAGTAATCAAGAGATTGCGGACGAGTTATTTATTGCACTGAAAACGGTAAAGACACATGTGAGTAATAT
ATTAAATAAATTGAATGTAAGTGACCGTACTCAGGCGGTTATTTATGCATTTAGGCATCAATTAACGAAGTGA

Upstream 100 bases:

>100_bases
TTCATGAAATTGGTGGGACACTTAAGGTACTTAGTTTCCCGGCCAAAGGTACACAAATAGAAGTAAAAGTACCGATTATG
ATTGAGAGAGGGGGAGAAGT

Downstream 100 bases:

>100_bases
TAAAGTGAAACTTTAATCAGTGGGGAGGTTGTTCCTCTCCCACTGATTATCAGCCCTCACCAATCGGACTTTTACGGGCA
GTTAATGCGGGATAAAGTAA

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEMDGVEATRAIIQEWPEAKIVV
VTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYDGETVLEPKVTGKMMSRMRQKKEQPLHDDLTEREFEILLLI
AEGKSNQEIADELFIALKTVKTHVSNILNKLNVSDRTQAVIYAFRHQLTK

Sequences:

>Translated_210_residues
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEMDGVEATRAIIQEWPEAKIVV
VTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYDGETVLEPKVTGKMMSRMRQKKEQPLHDDLTEREFEILLLI
AEGKSNQEIADELFIALKTVKTHVSNILNKLNVSDRTQAVIYAFRHQLTK
>Mature_210_residues
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEMDGVEATRAIIQEWPEAKIVV
VTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYDGETVLEPKVTGKMMSRMRQKKEQPLHDDLTEREFEILLLI
AEGKSNQEIADELFIALKTVKTHVSNILNKLNVSDRTQAVIYAFRHQLTK

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=33.175355450237, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=36.4077669902913, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=37.6811594202899, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=32, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786747, Length=208, Percent_Identity=29.3269230769231, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=23.1884057971014, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=37.5, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23631; Mature: 23631

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCEEEHHHHHHHH
DGVEATRAIIQEWPEAKIVVVTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHCCHHHHHHHHHHHCC
GETVLEPKVTGKMMSRMRQKKEQPLHDDLTEREFEILLLIAEGKSNQEIADELFIALKTV
CCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILNKLNVSDRTQAVIYAFRHQLTK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKVLLVDDHEMVRMGVSAYLSTQPDIEVVGEAENGRKGSELALQLRPDIILMDLVMDEM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCEEEHHHHHHHH
DGVEATRAIIQEWPEAKIVVVTSFLDDEKLYPVIEAGATSYLLKTSRASDIADAVRATYD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHCCHHHHHHHHHHHCC
GETVLEPKVTGKMMSRMRQKKEQPLHDDLTEREFEILLLIAEGKSNQEIADELFIALKTV
CCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILNKLNVSDRTQAVIYAFRHQLTK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]