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The map label for this gene is liaS [H]

Identifier: 218232183

GI number: 218232183

Start: 1430203

End: 1431258

Strand: Direct

Name: liaS [H]

Synonym: BCB4264_A1494

Alternate gene names: 218232183

Gene position: 1430203-1431258 (Clockwise)

Preceding gene: 218231170

Following gene: 218232967

Centisome position: 26.39

GC content: 37.59

Gene sequence:

>1056_bases
ATGAAGAAACAAAAAAATATTTCGTGGATGTATATTCGTTATTCTATGTTGTCCTCTGTTAGTATCGCACTCATTTGTAC
AATTGTATATGTATGGAAAAGCGAGCAACAAGTGTATGATTTATTATGGAAGGAATCCATCGCTTCTGTTCCAATTGGCT
TGTTTATTATGACTACAAGTTTACTTATCGGGGGAATTGTAGGATATGCAATCGGTTATTATATAGAGCAGCGTATACAA
GGATTAAATACTTTTCTATTTGAAGTAGAGCGAGGGAATTTTCCGAGCGATGTTTCGTTTACTGCAGATGATGAATTTCA
TGAAGTGGAACGAAAAGTAATCGTTCTGGCTCGTCGATTAGAGGAGCAAGCTGGACTCTTTCAAAAAGTAACGAATGAAC
GAGCTCATTGGAATGAAGAGATGAGACAAGAAGCGATTTCTCAAGAAAGGCATCGTTTAGCAAGGGAGTTGCATGATTCA
GTAAGTCAGCAGCTTTTCGCAATGTCGATGATGATGTCAGCGATTAATGAACAAATAACTGAATTTCCAGATGTGACAAA
AAAACAATTGCAACTTGTTGAAAATATGGTTGTAAATGCTCAATCAGAAATGAGAGCATTGCTCCTTCATTTACGTCCTG
TACAATTGGAAGGTAAGAAACTGACAGAAGGTATAGAGGAATTATTAACGGAATTATCGCGAAAACAACATATGAAAATT
GAATGGCTTATTGAGCCAATAGAATTAAAAAAAGGTGTAGAAGATCATTTATTCCGTATTGTGCAAGAAGCGCTATCAAA
CACGTTGCGACACGCGAAGGCAAAGAAAACAGAAGTGCGCCTTCGAAAAATTGATCAATATGCGATTTTAAAAATCATTG
ATGACGGCGTTGGGTTTGAAGTAGGGGTTAATAAGGCTGGTTCATATGGCCTAAGATCGATGCAAGAGCGTGTTCATGAA
ATTGGTGGGACACTTAAGGTACTTAGTTTCCCGGCCAAAGGTACACAAATAGAAGTAAAAGTACCGATTATGATTGAGAG
AGGGGGAGAAGTATGA

Upstream 100 bases:

>100_bases
GCAATGTTACATTTAGAACAGAGCAGTATAAAGAGGCTCCTCGACGTATTAAAATTATTTCTTCGCTTGTCGTAGGCGAC
ACGGAAGTGAGGAAAGTATA

Downstream 100 bases:

>100_bases
TAAAAGTATTGCTTGTCGATGATCATGAAATGGTTCGTATGGGTGTATCGGCATATTTATCGACACAGCCAGATATTGAA
GTAGTAGGGGAAGCTGAGAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIGGIVGYAIGYYIEQRIQ
GLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDS
VSQQLFAMSMMMSAINEQITEFPDVTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
EWLIEPIELKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIDQYAILKIIDDGVGFEVGVNKAGSYGLRSMQERVHE
IGGTLKVLSFPAKGTQIEVKVPIMIERGGEV

Sequences:

>Translated_351_residues
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIGGIVGYAIGYYIEQRIQ
GLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDS
VSQQLFAMSMMMSAINEQITEFPDVTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
EWLIEPIELKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIDQYAILKIIDDGVGFEVGVNKAGSYGLRSMQERVHE
IGGTLKVLSFPAKGTQIEVKVPIMIERGGEV
>Mature_351_residues
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIGGIVGYAIGYYIEQRIQ
GLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDS
VSQQLFAMSMMMSAINEQITEFPDVTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
EWLIEPIELKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIDQYAILKIIDDGVGFEVGVNKAGSYGLRSMQERVHE
IGGTLKVLSFPAKGTQIEVKVPIMIERGGEV

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=203, Percent_Identity=29.064039408867, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082311, Length=196, Percent_Identity=32.6530612244898, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788812, Length=205, Percent_Identity=26.3414634146341, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40378; Mature: 40378

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTS
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LLIGGIVGYAIGYYIEQRIQGLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDSVSQQLFAMSMMMSAINEQIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFPDVTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
EWLIEPIELKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIDQYAILKIIDDGVGFE
HEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VGVNKAGSYGLRSMQERVHEIGGTLKVLSFPAKGTQIEVKVPIMIERGGEV
ECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEEEECCCCC
>Mature Secondary Structure
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTS
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LLIGGIVGYAIGYYIEQRIQGLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDSVSQQLFAMSMMMSAINEQIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFPDVTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
EWLIEPIELKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIDQYAILKIIDDGVGFE
HEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VGVNKAGSYGLRSMQERVHEIGGTLKVLSFPAKGTQIEVKVPIMIERGGEV
ECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]