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The map label for this gene is yaaO [H]

Identifier: 218232811

GI number: 218232811

Start: 34505

End: 35926

Strand: Direct

Name: yaaO [H]

Synonym: BCB4264_A0034

Alternate gene names: 218232811

Gene position: 34505-35926 (Clockwise)

Preceding gene: 218232226

Following gene: 218231073

Centisome position: 0.64

GC content: 35.65

Gene sequence:

>1422_bases
ATGAATCAAAATCGTATGCCTTTATATGAGGCGTTAATAGAGTTTAAAGAAAGAGGGCCGTTATCCTTTCATGTTCCTGG
TCATAAGAATGGTTTAAACTTCCCTCAGGAGGCAATAAGAGAGTTTAAAGGTATTCTATCTATTGATGTAACTGAATTAG
CAGGACTAGATGATTTGCATAGTCCGTTTGAATGTATAGATGAGGCACAACAATTATTGGCTGAAGTTTATAATACAAAG
AGAAGTTATTTTTTAATTAATGGTTCAACAGTCGGGAACTTAGCAATGATTTTGTCTTGTTGCGGGGAACATGATATTGT
TCTTGTACAAAGAAATTGTCATAAATCAATTATTAATGGTTTAAAACTTGCGGGAGCAAATCCCATTTTTTTAGATCCAT
GGATTGATGAAGCTTATAACGTACCAGTGGGAGTTCGTAATGAAATTATTAAGAACGCAATTGGAAAGTATCCAAATGCG
AAAGCACTTATTTTAACGCATCCTAATTATTATGGTATGGGGATGGATTTAGAGGCAAGCATCGCTTTTGCACATGCACA
TAAAATCCCTGTTTTGGTAGATGAGGCACATGGGGCACATCTATGTTTGGGAGAACCATTCCCGAAGTCAGCATTAACAT
ATGGTGCAGATATTGTAGTTCATTCTGCACATAAAACATTACCCGCAATGACAATGGGTTCCTATTTACATATAAATAGT
CATTTAGTAGACGAAGATAAGGTTACTACTTATTTAAGTATGCTACAATCTAGTAGTCCATCGTATCCAATTATGGCTTC
CTTGGATATAGCACGCTTTACAATGGCGCGTATAAAAGAAGAAGGTCACAGTGAAATTGTTGAGTTTTTACGAAGATTTA
AGGAACAATTACGTTCTATTCCCCAAATCGCCATTTTAGAATATCCGTTGCAAGATGAGTTAAAGGTCACCGTACAAACT
CGTTGTCAGTTATCAGGGTACGAATTACAGTCTGTATTTGAAAAGGTCGGTATATATACAGAGATGGCTGATCCGTACAA
TGTCCTATTTATTTTACCGTTACAAGTAAATGAGGGATATATGAAGGTTATAGAGATGATCCGAGTAGCATTGCAACATT
ATGAGGTGAAAGACAAGAGGGAATCTATTCGTTATACTTATAATGGTGAATTCTCTCTTTTACCGTATACGTATAAACAA
CTAGACGGATATGAAACAAAGATAGTATCTATAGAAGATGCTGTTGGAATGATAGCGGCGGAAATGGTGATTCCGTATCC
ACCTGGAATTCCATTAATTATGTATGGAGAGAGAATTACTTCAGAGCATAAAGAGCAAATTATGTATTTAGAAAGAGCAG
GAGCTCGTTTTCAAGGTAATACGAAATATATGAAAGTGTATGATATAGAAAGTAGGTTTTAG

Upstream 100 bases:

>100_bases
TTCGAAAATTAGAAGTATCATATTTTTAAATAGGCATATGCCTATTATTTTTTTTGTTCTGTATAATAGATAGAGAATGA
ATAATAAGGAAGAGATGTAG

Downstream 100 bases:

>100_bases
GATGAAGGGATTATTTGTAACAATTGAGGGCCCAGAAGGTTCAGGTAAAACAACGTTAATTAAAAGCTTATTGCCATATT
TTGAACAAAAAGCACAAAAG

Product: Orn/Lys/Arg decarboxylase family protein

Products: 1,5-Diaminopentane; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNQNRMPLYEALIEFKERGPLSFHVPGHKNGLNFPQEAIREFKGILSIDVTELAGLDDLHSPFECIDEAQQLLAEVYNTK
RSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGVRNEIIKNAIGKYPNA
KALILTHPNYYGMGMDLEASIAFAHAHKIPVLVDEAHGAHLCLGEPFPKSALTYGADIVVHSAHKTLPAMTMGSYLHINS
HLVDEDKVTTYLSMLQSSSPSYPIMASLDIARFTMARIKEEGHSEIVEFLRRFKEQLRSIPQIAILEYPLQDELKVTVQT
RCQLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNEGYMKVIEMIRVALQHYEVKDKRESIRYTYNGEFSLLPYTYKQ
LDGYETKIVSIEDAVGMIAAEMVIPYPPGIPLIMYGERITSEHKEQIMYLERAGARFQGNTKYMKVYDIESRF

Sequences:

>Translated_473_residues
MNQNRMPLYEALIEFKERGPLSFHVPGHKNGLNFPQEAIREFKGILSIDVTELAGLDDLHSPFECIDEAQQLLAEVYNTK
RSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGVRNEIIKNAIGKYPNA
KALILTHPNYYGMGMDLEASIAFAHAHKIPVLVDEAHGAHLCLGEPFPKSALTYGADIVVHSAHKTLPAMTMGSYLHINS
HLVDEDKVTTYLSMLQSSSPSYPIMASLDIARFTMARIKEEGHSEIVEFLRRFKEQLRSIPQIAILEYPLQDELKVTVQT
RCQLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNEGYMKVIEMIRVALQHYEVKDKRESIRYTYNGEFSLLPYTYKQ
LDGYETKIVSIEDAVGMIAAEMVIPYPPGIPLIMYGERITSEHKEQIMYLERAGARFQGNTKYMKVYDIESRF
>Mature_473_residues
MNQNRMPLYEALIEFKERGPLSFHVPGHKNGLNFPQEAIREFKGILSIDVTELAGLDDLHSPFECIDEAQQLLAEVYNTK
RSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIINGLKLAGANPIFLDPWIDEAYNVPVGVRNEIIKNAIGKYPNA
KALILTHPNYYGMGMDLEASIAFAHAHKIPVLVDEAHGAHLCLGEPFPKSALTYGADIVVHSAHKTLPAMTMGSYLHINS
HLVDEDKVTTYLSMLQSSSPSYPIMASLDIARFTMARIKEEGHSEIVEFLRRFKEQLRSIPQIAILEYPLQDELKVTVQT
RCQLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNEGYMKVIEMIRVALQHYEVKDKRESIRYTYNGEFSLLPYTYKQ
LDGYETKIVSIEDAVGMIAAEMVIPYPPGIPLIMYGERITSEHKEQIMYLERAGARFQGNTKYMKVYDIESRF

Specific function: Appears To Play A Role In pH Homeostasis By Consuming Protons And Neutralizing The Acidic By-Products Of Carbohydrate Fermentation. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1790573, Length=567, Percent_Identity=23.1040564373898, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1786384, Length=552, Percent_Identity=21.9202898550725, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI221142684, Length=418, Percent_Identity=23.9234449760766, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786909, Length=140, Percent_Identity=32.8571428571429, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082193, Length=134, Percent_Identity=31.3432835820896, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18 [C]

Molecular weight: Translated: 53598; Mature: 53598

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNRMPLYEALIEFKERGPLSFHVPGHKNGLNFPQEAIREFKGILSIDVTELAGLDDLH
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCEEEEHHHHCCCCCCC
SPFECIDEAQQLLAEVYNTKRSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIING
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCC
LKLAGANPIFLDPWIDEAYNVPVGVRNEIIKNAIGKYPNAKALILTHPNYYGMGMDLEAS
CEECCCCCEEEECCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCE
IAFAHAHKIPVLVDEAHGAHLCLGEPFPKSALTYGADIVVHSAHKTLPAMTMGSYLHINS
EEEEECCCCCEEEECCCCCEEEECCCCCCHHHHCCCEEEEECCHHCCCHHHCCCEEEECC
HLVDEDKVTTYLSMLQSSSPSYPIMASLDIARFTMARIKEEGHSEIVEFLRRFKEQLRSI
CCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PQIAILEYPLQDELKVTVQTRCQLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNEGY
CCEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHCCEEECCCCCEEEEEEEEEECCHH
MKVIEMIRVALQHYEVKDKRESIRYTYNGEFSLLPYTYKQLDGYETKIVSIEDAVGMIAA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCHHHHHCCCCCEEEEEEHHHHHHHHH
EMVIPYPPGIPLIMYGERITSEHKEQIMYLERAGARFQGNTKYMKVYDIESRF
HEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MNQNRMPLYEALIEFKERGPLSFHVPGHKNGLNFPQEAIREFKGILSIDVTELAGLDDLH
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCEEEEHHHHCCCCCCC
SPFECIDEAQQLLAEVYNTKRSYFLINGSTVGNLAMILSCCGEHDIVLVQRNCHKSIING
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCC
LKLAGANPIFLDPWIDEAYNVPVGVRNEIIKNAIGKYPNAKALILTHPNYYGMGMDLEAS
CEECCCCCEEEECCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCE
IAFAHAHKIPVLVDEAHGAHLCLGEPFPKSALTYGADIVVHSAHKTLPAMTMGSYLHINS
EEEEECCCCCEEEECCCCCEEEECCCCCCHHHHCCCEEEEECCHHCCCHHHCCCEEEECC
HLVDEDKVTTYLSMLQSSSPSYPIMASLDIARFTMARIKEEGHSEIVEFLRRFKEQLRSI
CCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PQIAILEYPLQDELKVTVQTRCQLSGYELQSVFEKVGIYTEMADPYNVLFILPLQVNEGY
CCEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHCCEEECCCCCEEEEEEEEEECCHH
MKVIEMIRVALQHYEVKDKRESIRYTYNGEFSLLPYTYKQLDGYETKIVSIEDAVGMIAA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCHHHHHCCCCCEEEEEEHHHHHHHHH
EMVIPYPPGIPLIMYGERITSEHKEQIMYLERAGARFQGNTKYMKVYDIESRF
HEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: H+; L-Lysine [C]

Specific reaction: H+ + L-Lysine --> 1,5-Diaminopentane + CO2 [C]

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]