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The map label for this gene is atoC [C]

Identifier: 218232734

GI number: 218232734

Start: 347096

End: 348463

Strand: Direct

Name: atoC [C]

Synonym: BCB4264_A0372

Alternate gene names: 218232734

Gene position: 347096-348463 (Clockwise)

Preceding gene: 218232749

Following gene: 218233344

Centisome position: 6.41

GC content: 36.33

Gene sequence:

>1368_bases
ATGGTTGCAGAAAAGGAACGAGTGTTAATGGATTTAAAAGATGTATTTGAATATGCGTTTGACGAAATTTTTGTTACAGA
TGAGCAAGGGATCGTTGTACGTGTAAATAGTACATGTGAAAGGCACTACCAACTAGCTGCAGAAGAGTTAGTTGGTAAGC
ATGTAAAAGAACTACAAAAGGATGGAATCTTTTATCCATCAGCGACATTAGAAGTGATTGAAAAAAAGAGGCCAATTGAA
CTCGTTCAAACTACAAAATCAGGAGAGTATTTACACGTTCGTACAAGGCCTGTTTTTGATGATGAGGGAAATTTAAGAAG
AGTGATTAGTTATTCTCGCGACCTTACTGAACTCTATCAATTACGTCAAAAGGTAGAGGAAATGGATAATCAGCTAAAAA
CATATAAAAAAGAATTAAGAGAAACATATGAGCATGAAGGACTTATTTTTAAAAGTCTAGCTATGCAAAAAATAGTCGAT
ACAATCAAAAAAGTATCTGTAGTGGATAGTACTGTTCTCGTTTTAGGCGAGACTGGAGTAGGGAAAAGTCGATTAGTACG
CCATTTACATGAAGTGAGTCACCGTAAGTATGAAAGTTTCTATGAAATTAATTGTGCGGCATTGCCAACTAATTTAATTG
AATCGGAGCTTTTTGGATATTCAGGTGGATCTTTTACAGGTGCGAATCGTGAAGGGAAAAAGGGACTATTAGAATCCGCG
CATAAAGGAACTCTTTTTTTAGATGAAATCGGTGAAATGCCGCTTGAAATTCAAGCGAAGCTTTTGCAAGTATTGCAAGA
AAAAACATTTCGTCCTATAGGCGGAAGAGAATTAAAAAAAGTGGATGTTCGAATTGTGGCGGCAACAAATAGAGATTTAA
GCGAGATGGTGAAACAAGGAACATTTCGGAAAGATTTATACTATCGTTTGAATGTCATTCCAATTGCAATTCCCCCGCTT
AGGGAAAGAACAGAAGATATTTTGCCGCTTATTTATCATTACTTGCAGCACTTTAATAAAAAGTACGGACGTGATGTGAA
ACTAGCGCCGAGTACGTTACAAATGTTTGTTGGATATCCGTGGGAAGGGAACAATAGAGAAATAGAGAATGTAATTGAGA
GAATTGTTATTACTGTTGATGATGTTGTAACGGTAGAGGATTTGCCACTCTCTATGCAAGAGGCTGCAGTTGAACAATCG
GGGCAAAGCCTTTATAAAATGCTGGAAGAGGTAGAGAGAAATATAATTCTTAAAGCGTATAAAACGTATGGATCAAGTTA
TAAAGTGGCTGAGTTTTTGCAAATTAGTCAATCTGCTGCTACTAGAAAAATTAAGAAGTTCATAGAGGAGGAAGAAAACA
TTGGATAA

Upstream 100 bases:

>100_bases
TAGCAAACATTCTAGCTGACTCTATGTTAAATGCAGAAAATTCACTTTATAATAAAGGTAGTGAAGTGGATCGATGGATT
TGAAGTTAGGGGTGGCTAGG

Downstream 100 bases:

>100_bases
AAAGGCGACTTTCGTAAGTATAGATAAAAAGGCGATGTATATAAATGGTGAGTGGATTACACTACAAGAACAAATTGAAG
TAAATAATCCTGCGACGAAG

Product: sensory box sigma-54 dependent DNA-binding response regulator

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MVAEKERVLMDLKDVFEYAFDEIFVTDEQGIVVRVNSTCERHYQLAAEELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSLAMQKIVD
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEVSHRKYESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSEMVKQGTFRKDLYYRLNVIPIAIPPL
RERTEDILPLIYHYLQHFNKKYGRDVKLAPSTLQMFVGYPWEGNNREIENVIERIVITVDDVVTVEDLPLSMQEAAVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLQISQSAATRKIKKFIEEEENIG

Sequences:

>Translated_455_residues
MVAEKERVLMDLKDVFEYAFDEIFVTDEQGIVVRVNSTCERHYQLAAEELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSLAMQKIVD
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEVSHRKYESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSEMVKQGTFRKDLYYRLNVIPIAIPPL
RERTEDILPLIYHYLQHFNKKYGRDVKLAPSTLQMFVGYPWEGNNREIENVIERIVITVDDVVTVEDLPLSMQEAAVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLQISQSAATRKIKKFIEEEENIG
>Mature_455_residues
MVAEKERVLMDLKDVFEYAFDEIFVTDEQGIVVRVNSTCERHYQLAAEELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSLAMQKIVD
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEVSHRKYESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSEMVKQGTFRKDLYYRLNVIPIAIPPL
RERTEDILPLIYHYLQHFNKKYGRDVKLAPSTLQMFVGYPWEGNNREIENVIERIVITVDDVVTVEDLPLSMQEAAVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLQISQSAATRKIKKFIEEEENIG

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=353, Percent_Identity=41.3597733711048, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=39.937106918239, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=48.0519480519481, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1789087, Length=243, Percent_Identity=47.3251028806584, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1790437, Length=334, Percent_Identity=37.4251497005988, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790299, Length=328, Percent_Identity=38.109756097561, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1787583, Length=303, Percent_Identity=35.6435643564356, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788905, Length=287, Percent_Identity=38.6759581881533, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786524, Length=364, Percent_Identity=34.3406593406593, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082152, Length=295, Percent_Identity=38.9830508474576, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=301, Percent_Identity=33.8870431893688, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI87081858, Length=463, Percent_Identity=27.4298056155508, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789828, Length=240, Percent_Identity=35.8333333333333, Blast_Score=158, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078 [H]

Pfam domain/function: PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52441; Mature: 52441

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAEKERVLMDLKDVFEYAFDEIFVTDEQGIVVRVNSTCERHYQLAAEELVGKHVKELQK
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DGIFYPSATLEVIEKKRPIELVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQ
CCCCCCCHHHHHHHCCCCHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHHH
LRQKVEEMDNQLKTYKKELRETYEHEGLIFKSLAMQKIVDTIKKVSVVDSTVLVLGETGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKSRLVRHLHEVSHRKYESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
CHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSEMVKQG
CCCCEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCHHHHHHCC
TFRKDLYYRLNVIPIAIPPLRERTEDILPLIYHYLQHFNKKYGRDVKLAPSTLQMFVGYP
CCHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHEECC
WEGNNREIENVIERIVITVDDVVTVEDLPLSMQEAAVEQSGQSLYKMLEEVERNIILKAY
CCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTYGSSYKVAEFLQISQSAATRKIKKFIEEEENIG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVAEKERVLMDLKDVFEYAFDEIFVTDEQGIVVRVNSTCERHYQLAAEELVGKHVKELQK
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DGIFYPSATLEVIEKKRPIELVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQ
CCCCCCCHHHHHHHCCCCHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHHH
LRQKVEEMDNQLKTYKKELRETYEHEGLIFKSLAMQKIVDTIKKVSVVDSTVLVLGETGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKSRLVRHLHEVSHRKYESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
CHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSEMVKQG
CCCCEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCHHHHHHCC
TFRKDLYYRLNVIPIAIPPLRERTEDILPLIYHYLQHFNKKYGRDVKLAPSTLQMFVGYP
CCHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHEECC
WEGNNREIENVIERIVITVDDVVTVEDLPLSMQEAAVEQSGQSLYKMLEEVERNIILKAY
CCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTYGSSYKVAEFLQISQSAATRKIKKFIEEEENIG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]