Click here to switch to the map view.

The map label for this gene is asnO2 [H]

Identifier: 218232672

GI number: 218232672

Start: 1673954

End: 1675777

Strand: Reverse

Name: asnO2 [H]

Synonym: BCB4264_A1767

Alternate gene names: 218232672

Gene position: 1675777-1673954 (Counterclockwise)

Preceding gene: 218235371

Following gene: 218232855

Centisome position: 30.92

GC content: 35.69

Gene sequence:

>1824_bases
ATGTGTGGAATTACAGGTTGGGTAGATTGGAAGGAAGACCTTTCAAATCAACATGTTATTTTAAAAAAGATGGCAAATAG
TATTGAGCATCGTGGTCCAGATGCTGAAGGATTTTGGTTTTCACCTCATGCGGCCTTTGCACACCGGCGTTTAATTGTAA
TTGATCCAGAAGGCGGTACACAGCCGAAGACATTCCGTGCTGGTGATTATACGTATGCTCTTACTTATAATGGAGAAATT
TATAATTTCCGTGAACTTAAAGAGCAGCTTCAAAAATGTGGGCATGCATTTGAAACCCATTCAGATACAGAAGTGTTACT
ACATGCTTATTTAGAATGGAAAGAAGACTGCGTGCGATATTTAAACGGAATTTTCGCTTTTGCCTTATGGGATGATCAGA
AGCAACAATTATTTTTAGCGCGTGATCATTTAGGTGTAAAACCACTCTTTTTTACAGAAAGAAATGGCAGTATTATTTTC
GGCTCTGAAATTAAAGCATTATTAGCGCATCCATCTGTCCCTGCTGAACTTGATGCGGGTGGAATAAATGAAATATTTGG
ATTAGGCTTATTTCGCACTCCAGGATGTGGCGTCTTTAAACATATTCAAGAAGTACGTGCTGGACATTCTATAACATTTA
CACGTCATAAAAAAGTAGTTACGAAGTATTGGAATTTAGAAAGTAAGTTCCATACAGACTCTATAGAAGATACATCTTCG
CATATTTTATCTATTTTACAAGATACAGTAAAAAGACAATTAATTGCCGATGTTCCTCTCGTTTGTATGTTATCAGGCGG
ATTAGACTCTAGCGGTATTACTGCACTTGCTGGTAAAGAATTTGCTGCTGAAAATAAAACACTTCATACGTATTCTGTTG
ACTTTGTAAATAGCGCAAAAGATTTCGAACTGACATTTGCTCGCACTGGTTTAGACGCACCTTGGGTAAAACGCGTTTCT
GAACATGTCAGGACAGCGCATCATGATATTATTGTGAATGCTGAAGAATTAGCAAATCACTTATTCGTTCCCCTTCATGC
AAAAGATTTACCGAGTGCTGGTGAAATGGAAACTTCACTATATTTACTATTTTGCGAAATGAAAAAAGATGCGACCGTAG
CTTTATCCGGTGAATCGGCTGATGAAGTATTCGGTGGATACCCTTGGTTTCATCAAGAAGAATTACTATATGTAGATAAG
TTTCCTTGGCTAACAAATTGGAAAAATACATCTCCTCTTCTACTAAATGAAGTAAGTAACCAATGTAATCTAGAAAACTA
TATTGATACACGATTCCAAGAAGCGATACTTGAAGTACCTACTCTTGAAGGGGAAAGTAAAAAATCCGCGAAACAACGCC
AAATGTTTTATTTATTTTTAACAAGATTCCTTCCTTTCTTACTTGACCGTAAAGATCGTATGAGTATGGCTGTTGGATTT
GAAGTTCGTGTACCGTTTTGCGATTATAGATTAGTTGAATATTTATGGAATGTTCCTTTCAACATAAAAAGTATTGATAA
TATTGAAAAAGGCATTTTACGTAGAGCACTACAACCAGCTTTACCTGACGATGTACGTAATAGAAGAAAAAGCGCTTATC
CAACTTCACAAGATCCACACTACTTACAAACAATCCGTAATTTAACACTTGATATGTGTAGTAATAAAAACAATCCTATT
TTCTCACTTATTAACCATTCCATCTTACTTTCAATTGCTGATCAAAGTAATAAAGAGATTAACAATTTTGAGGCAAGAAG
TGCCATGGAGTATATGCTACAAGTAAATGAATGGTTAAAAACATATCATATTCATATTGCTTAA

Upstream 100 bases:

>100_bases
AATTTAATTCATGTAGTAGACTATTTATATAGTCACTTTTTAACTTAGTGTACATTTTATTTCGTTAATCTACTATTTTA
ATTTAGGAGATGAGATGTGT

Downstream 100 bases:

>100_bases
CAAAAAGGAGTTCAAGGATAAAAGCCTTGAACTCCTTTTATATTAATTAGCACATTGTTTATTATCAGTATCAATAGACC
ACCCAATAATCGTGGCACAA

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MCGITGWVDWKEDLSNQHVILKKMANSIEHRGPDAEGFWFSPHAAFAHRRLIVIDPEGGTQPKTFRAGDYTYALTYNGEI
YNFRELKEQLQKCGHAFETHSDTEVLLHAYLEWKEDCVRYLNGIFAFALWDDQKQQLFLARDHLGVKPLFFTERNGSIIF
GSEIKALLAHPSVPAELDAGGINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRHKKVVTKYWNLESKFHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVRTAHHDIIVNAEELANHLFVPLHAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLNEVSNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALQPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLDMCSNKNNPI
FSLINHSILLSIADQSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA

Sequences:

>Translated_607_residues
MCGITGWVDWKEDLSNQHVILKKMANSIEHRGPDAEGFWFSPHAAFAHRRLIVIDPEGGTQPKTFRAGDYTYALTYNGEI
YNFRELKEQLQKCGHAFETHSDTEVLLHAYLEWKEDCVRYLNGIFAFALWDDQKQQLFLARDHLGVKPLFFTERNGSIIF
GSEIKALLAHPSVPAELDAGGINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRHKKVVTKYWNLESKFHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVRTAHHDIIVNAEELANHLFVPLHAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLNEVSNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALQPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLDMCSNKNNPI
FSLINHSILLSIADQSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA
>Mature_607_residues
MCGITGWVDWKEDLSNQHVILKKMANSIEHRGPDAEGFWFSPHAAFAHRRLIVIDPEGGTQPKTFRAGDYTYALTYNGEI
YNFRELKEQLQKCGHAFETHSDTEVLLHAYLEWKEDCVRYLNGIFAFALWDDQKQQLFLARDHLGVKPLFFTERNGSIIF
GSEIKALLAHPSVPAELDAGGINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRHKKVVTKYWNLESKFHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVRTAHHDIIVNAEELANHLFVPLHAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLNEVSNQCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALQPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLDMCSNKNNPI
FSLINHSILLSIADQSNKEINNFEARSAMEYMLQVNEWLKTYHIHIA

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=555, Percent_Identity=27.9279279279279, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI168229252, Length=555, Percent_Identity=27.9279279279279, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI168229250, Length=555, Percent_Identity=27.9279279279279, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI296010848, Length=528, Percent_Identity=27.8409090909091, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI296010852, Length=455, Percent_Identity=26.1538461538462, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI296010850, Length=455, Percent_Identity=26.1538461538462, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI1786889, Length=414, Percent_Identity=30.4347826086957, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71993933, Length=538, Percent_Identity=26.3940520446097, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=538, Percent_Identity=26.3940520446097, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25147560, Length=326, Percent_Identity=25.1533742331288, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=431, Percent_Identity=27.8422273781903, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6325403, Length=420, Percent_Identity=28.3333333333333, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45553209, Length=371, Percent_Identity=29.6495956873315, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69532; Mature: 69532

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVDWKEDLSNQHVILKKMANSIEHRGPDAEGFWFSPHAAFAHRRLIVIDPEGGT
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELKEQLQKCGHAFETHSDTEVLLHAYLEWKEDCVRY
CCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQQLFLARDHLGVKPLFFTERNGSIIFGSEIKALLAHPSVPAELDAG
HHHHEEEEEECCCHHHEEEEHHCCCCCEEEEECCCCCEEECCHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRHKKVVTKYWNLESKFHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVRTAHHDIIVNAEELANHLFVPLHAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLNEVSN
EEEEEECCCCCEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
QCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALQPALPDDVRNRRKSAYPTSQDPH
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCH
YLQTIRNLTLDMCSNKNNPIFSLINHSILLSIADQSNKEINNFEARSAMEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC
>Mature Secondary Structure
MCGITGWVDWKEDLSNQHVILKKMANSIEHRGPDAEGFWFSPHAAFAHRRLIVIDPEGGT
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELKEQLQKCGHAFETHSDTEVLLHAYLEWKEDCVRY
CCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQQLFLARDHLGVKPLFFTERNGSIIFGSEIKALLAHPSVPAELDAG
HHHHEEEEEECCCHHHEEEEHHCCCCCEEEEECCCCCEEECCHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIQEVRAGHSITFTRHKKVVTKYWNLESKFHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVRTAHHDIIVNAEELANHLFVPLHAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLNEVSN
EEEEEECCCCCEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
QCNLENYIDTRFQEAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALQPALPDDVRNRRKSAYPTSQDPH
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCH
YLQTIRNLTLDMCSNKNNPIFSLINHSILLSIADQSNKEINNFEARSAMEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]