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The map label for this gene is lysS [H]

Identifier: 218232500

GI number: 218232500

Start: 80466

End: 81965

Strand: Direct

Name: lysS [H]

Synonym: BCB4264_A0084

Alternate gene names: 218232500

Gene position: 80466-81965 (Clockwise)

Preceding gene: 218231778

Following gene: 218235794

Centisome position: 1.48

GC content: 37.13

Gene sequence:

>1500_bases
ATGGATAACATGAACCACGAAGAATTAAACGACCAATTGATTGTTCGTCGTGAAAAGCTACATAATTTACGTGAACAAGG
AATTGACCCGTTCGGTAAAAGATTTGAACGCACAAATTCAACAACTGATTTAGTGAGTCTATATGGAGAGTTCTCTAAGG
AAGAATTAGAGGAGAAGGAAATCGCTGTTTCTATCGCTGGTCGTATTATGACAAAACGCGGAAAAGGGAAAGCTGGATTT
GCACACGTTCAAGATTTACATGGACAAGTTCAAATTTATGTTCGTAAAGATGCTGTTGGAGATGACGAGTACGAATTATT
TAAAACAGCAGATTTAGGTGATTTAGTAGGTATTGAAGGTAAAGTGTTCAAAACAAACGTTGGAGAACTTTCAGTAAAAG
CAACAGGCTTTACGCTATTAACAAAATCTCTTCGTCCATTACCAGATAAATACCATGGATTAAAAGATGTTGAACAACGC
TACCGTCAACGTTACTTAGACTTAATTACAAGTATGGAAAGTCGTGAAACGTTCGTTACTCGTAGTAAAATCATTCGTGA
GATGAGAAGATACCTAGATGACAACGGTTATCTTGAAGTGGAAACACCTATGATGCATGCAATTGCAGGTGGAGCTTCTG
CGCGTCCTTTCACTACACATCATAATGCGTTAGATATGGAATTATATATGCGTATCGCAATTGAGCTACATTTAAAGCGT
CTTATTGTGGGTGGTTTGGAAAAAGTATATGAGATCGGCCGTGTATTCCGTAATGAGGGTGTATCAACTCGTCATAACCC
TGAGTTTACGATGATTGAATTATATGAAGCATACGCTGATTATAAAGATATTATGAAGTTAACAGAAGATATGGTTGCTC
ATATCGCGAAAAAAGTACTGGGTACAACAACAATCCAGTATGGTGATTATGAAATTAATCTAGAACCAGAATGGACACGT
CTTCATATGGTAGATGCGATTAAGCAATACTCTGGAGCTGATTTCTGGAATCCGATGAGTGTAGAAGAAGCACGTGAGCT
TGCAAAAGAACATAATGTAGAAATTAAAGATACAATGGAAGTTGGTCATATTATAAATGAATTCTTCGAACAAAAAGTAG
AAGATAAATTAATCCAACCAACATTTATTTACGGTCATCCGGTAGAAATCTCGCCGCTTGCGAAAAAGAATGATGAAGAT
CCAAGATTTACAGATCGTTTCGAATTATTTATCGTTGCGCGTGAACATGCAAATGCATTCACTGAGTTAAATGATCCAAT
CGATCAAAAAGAACGTTTTGAAGCGCAATTAAAAGAGCGCGAACAAGGTAATGATGAAGCTCATATGATGGATGACGATT
ATATTGAAGCTCTTGAGTACGGTATGCCTCCTACGGGTGGATTAGGAATCGGTATTGATCGTCTAGTTATGTTATTAACA
AATGCACCATCTATTCGTGATGTATTATTATTCCCTGCTATGCGCCATAAACAAGACTAA

Upstream 100 bases:

>100_bases
AAGAGCTGCCAGTTAGTTGCTGGCAGTTTTTCTAGTTGAGTAGAAAATGCTATACTGTATATATTGTAAAATTAAATAGA
GCTGGAGTGATATCAATATC

Downstream 100 bases:

>100_bases
GCTTTGGAGATTTCTCCAAAGCTTTTTTTATTTGAGCATGAGTTGCGTGGGTATAGTAAATCTTGATAGTGCGAGAAATT
ATATTTATATAGAAGAAATG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MDNMNHEELNDQLIVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKEIAVSIAGRIMTKRGKGKAGF
AHVQDLHGQVQIYVRKDAVGDDEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTEDMVAHIAKKVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKQYSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

Sequences:

>Translated_499_residues
MDNMNHEELNDQLIVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKEIAVSIAGRIMTKRGKGKAGF
AHVQDLHGQVQIYVRKDAVGDDEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTEDMVAHIAKKVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKQYSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD
>Mature_499_residues
MDNMNHEELNDQLIVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKEIAVSIAGRIMTKRGKGKAGF
AHVQDLHGQVQIYVRKDAVGDDEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTEDMVAHIAKKVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKQYSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=504, Percent_Identity=41.8650793650794, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=42.0841683366733, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI45439306, Length=474, Percent_Identity=24.6835443037975, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI40789249, Length=281, Percent_Identity=24.5551601423488, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=52.8571428571429, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1790571, Length=493, Percent_Identity=52.1298174442191, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI87082379, Length=329, Percent_Identity=32.8267477203647, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788173, Length=298, Percent_Identity=25.503355704698, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=491, Percent_Identity=46.4358452138493, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17535927, Length=491, Percent_Identity=46.4358452138493, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71994340, Length=491, Percent_Identity=46.4358452138493, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551876, Length=346, Percent_Identity=24.2774566473988, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=272, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=508, Percent_Identity=43.3070866141732, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324256, Length=483, Percent_Identity=32.0910973084886, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323011, Length=350, Percent_Identity=25.7142857142857, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=318, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640851, Length=506, Percent_Identity=42.0948616600791, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24640849, Length=506, Percent_Identity=42.0948616600791, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24584738, Length=279, Percent_Identity=26.1648745519713, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922416, Length=376, Percent_Identity=22.8723404255319, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57640; Mature: 57640

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNMNHEELNDQLIVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IAVSIAGRIMTKRGKGKAGFAHVQDLHGQVQIYVRKDAVGDDEYELFKTADLGDLVGIEG
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCCHHCCCCC
KVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQRYRQRYLDLITSMESRETFVT
EEEEECCCCEEEEECCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
HHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTEDMVAHIAKKVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTTIQYGDYEINLEPEWTRLHMVDAIKQYSGADFWNPMSVEEARELAKEHNVEIKDTME
HCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDEDPRFTDRFELFIVAREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEEEHHCCHH
TELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
NAPSIRDVLLFPAMRHKQD
CCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDNMNHEELNDQLIVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IAVSIAGRIMTKRGKGKAGFAHVQDLHGQVQIYVRKDAVGDDEYELFKTADLGDLVGIEG
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCCHHCCCCC
KVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQRYRQRYLDLITSMESRETFVT
EEEEECCCCEEEEECCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
HHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTEDMVAHIAKKVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTTIQYGDYEINLEPEWTRLHMVDAIKQYSGADFWNPMSVEEARELAKEHNVEIKDTME
HCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDEDPRFTDRFELFIVAREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEEEHHCCHH
TELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
NAPSIRDVLLFPAMRHKQD
CCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA