Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 218231778

GI number: 218231778

Start: 79308

End: 80306

Strand: Direct

Name: dus1 [H]

Synonym: BCB4264_A0083

Alternate gene names: 218231778

Gene position: 79308-80306 (Clockwise)

Preceding gene: 218235456

Following gene: 218232500

Centisome position: 1.46

GC content: 39.94

Gene sequence:

>999_bases
TTGTTAAAGATTGCAAATATCGAGATGAAAAATCCGGTTGTACTAGCACCGATGGCGGGAGTGTGTAACTCTGCATTCCG
TTTAACAGTAAAAGAATTTGGTGCCGGCTTAGTTTGTGCTGAAATGGTAAGTGATAAGGCAATATTACTTAATAATAAAA
GAACATTAGATATGTTATATATCGACGAGAGAGAAAAGCCATTAAGTTTACAAATTTTTGGTGGAGAGAAAGAAACTCTT
GTAGATGCTGCGAAATATGTAGATAAATATACGACAGCAGACATAATTGATATTAATATGGGTTGTCCAGTGCCGAAAAT
CGTTAAATGTGATGCGGGGGCAAGGTGGCTTTTAGATCCGAATAAAATATATGAAATGGTAGCAGCGGTTGTAGATGCTG
TTGAAAAGCCGGTTACAGTTAAAATGCGTATCGGTTGGGATGAAGAACATATTTTCGCAATAGAAAATGCTAGGGCTGTT
GAGCGTGCTGGTGGCCAAGCTGTGGCGGTTCACGGACGTACACGAGTGCAAATGTATGAAGGAAAAGCGGATTGGGATAT
TATTAAACAAGTAAAACAATCTGTAAATATCCCGGTTATCGGAAATGGTGATGTCGCAACGCCGCAAGATGCAAAGCGTA
TGCTTGATGAAATTGGTGTAGATGGTGTTATGATTGGTCGTGCGGCTCTTGGAGATCCGTGGATGATTTATCGTACGGTA
AAGTATTTGGAGACGGGTGAATTAATGCCGGAACCAACAGTGCGCGAGAAAATTGATGTATGTATGTTACATCTAGATCG
TCTTATTGACTTAAAGAACGAAAGTGTCGCTGTAAGAGAGATGAGAAAACATGCAGCTTGGTATTTAAAAGGTGTTCGTG
GTAATGCGAGTGTGCGTAATGGTATTAATGCTTGTAACACACGGGAAGACCTTGCGAATGTGTTAGGTACATTTGTAGAA
GAAGTGGAAGCGAAACAACAAACAATTTACGTTGGTTAA

Upstream 100 bases:

>100_bases
GATCGCCTTCTCAAGATTTTGTAGTAGAAGTTGCTAAGGCATTAAATGTTTCGATAGAGGAATTAATGCCAAAGTGATTG
TACAGGAAGGAGTAGGTTGA

Downstream 100 bases:

>100_bases
TAATGGACATAGGAGATTGACATTCTTCTATACACTTCCTATAATTACGTGAAAGAAAGAAGAGCTGCCAGTTAGTTGCT
GGCAGTTTTTCTAGTTGAGT

Product: putative TIM-barrel protein, NifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VDAAKYVDKYTTADIIDINMGCPVPKIVKCDAGARWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEEHIFAIENARAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQSVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESVAVREMRKHAAWYLKGVRGNASVRNGINACNTREDLANVLGTFVE
EVEAKQQTIYVG

Sequences:

>Translated_332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VDAAKYVDKYTTADIIDINMGCPVPKIVKCDAGARWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEEHIFAIENARAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQSVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESVAVREMRKHAAWYLKGVRGNASVRNGINACNTREDLANVLGTFVE
EVEAKQQTIYVG
>Mature_332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VDAAKYVDKYTTADIIDINMGCPVPKIVKCDAGARWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEEHIFAIENARAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQSVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESVAVREMRKHAAWYLKGVRGNASVRNGINACNTREDLANVLGTFVE
EVEAKQQTIYVG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=30.0429184549356, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=30.3030303030303, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=30.8370044052863, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=30.9734513274336, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=28.9156626506024, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=38.125, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1788462, Length=254, Percent_Identity=37.7952755905512, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693211, Length=227, Percent_Identity=27.7533039647577, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=228, Percent_Identity=30.7017543859649, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=28.8065843621399, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=29.7674418604651, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=29.5546558704453, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=30.1724137931034, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=27.0742358078603, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=32.6693227091633, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=32.6693227091633, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585320, Length=241, Percent_Identity=33.195020746888, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI45549423, Length=240, Percent_Identity=30.4166666666667, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19921524, Length=306, Percent_Identity=26.1437908496732, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36861; Mature: 36861

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLY
CEEEEEEECCCCEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEEECCCEEEEEE
IDEREKPLSLQIFGGEKETLVDAAKYVDKYTTADIIDINMGCPVPKIVKCDAGARWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEECH
NKIYEMVAAVVDAVEKPVTVKMRIGWDEEHIFAIENARAVERAGGQAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GKADWDIIKQVKQSVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
CCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHH
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESVAVREMRKHAAWYLKGVRGNASVRN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCHHHC
GINACNTREDLANVLGTFVEEVEAKQQTIYVG
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLY
CEEEEEEECCCCEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEEECCCEEEEEE
IDEREKPLSLQIFGGEKETLVDAAKYVDKYTTADIIDINMGCPVPKIVKCDAGARWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEECH
NKIYEMVAAVVDAVEKPVTVKMRIGWDEEHIFAIENARAVERAGGQAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GKADWDIIKQVKQSVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
CCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHH
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESVAVREMRKHAAWYLKGVRGNASVRN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCHHHC
GINACNTREDLANVLGTFVEEVEAKQQTIYVG
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]