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The map label for this gene is nrdE [H]

Identifier: 218232459

GI number: 218232459

Start: 1356945

End: 1358993

Strand: Direct

Name: nrdE [H]

Synonym: BCB4264_A1405

Alternate gene names: 218232459

Gene position: 1356945-1358993 (Clockwise)

Preceding gene: 218234446

Following gene: 218233288

Centisome position: 25.04

GC content: 36.26

Gene sequence:

>2049_bases
ATGCAGGACGGTTTTTATCAGCTTCATAAAGATAAAGAGGCATTAGAAGTCTTTATGGAAGAAGCTAGAGAGAATACCGT
TCCTTTTAATAGCGTGGCAGAGCGAATGGAGTATATGAAAGAACATGATTACTATTACAACGTTCTGGACGAGTATAGCT
TGGAGGAAGTAGAGGAAGTATATAACATCGCTTATGGTGAAAACTTCGAGTTCCAATCTTATATGGCAGCATCTAAGTTC
TACAAAGATTATGCGTTAAAAACGAATGATCAAAAACAATACTTAGAAAGCTATGAAGATCGTGTAGCAATTGTTTCATT
ATACTTAGGACGCGGTGATGTTTCGAAGGCAAGACAATTCGCAAGCATGATTGTAAAACAAAACTACCAACCAGCGACAC
CAACCTTTTTAAATGCGGGAAGAAGCAGAAGAGGAGAAATGGTGTCTTGTTTCTTGTTAGAGATGGACGATAGCTTAAAT
TCGATCGGCTTTAACATTAATACTGCGATGCAATTATCGAAAATCGGCGGGGGAGTAGCTTTAAACTTATCTAAGCTACG
CGCACGCGGTGAGCAAATTAAAGGTATCGACAACGCTGCAAGTGGTGTAGTACCTGTTATGAAATTGCTTGAAGATTCGT
TTTCATATGCGAATCAGCTTGGCCAACGAAAAGGTGCCGGTGCAGTATATTTAAACATTTTCCATTGGGATATTATTGAA
TTCCTTGATACAAAAAAAATAAATGCCGATGAGAAGAGCCGTATTCAGTCTCTATCAATCGGAATTATCGTTCCAAGTAA
GTTCTTTGAGCTTGCTGAGAAAAACGAACCTTTCCATGTTTTCGCGCCTTATACGGTTTATAAAGAATATGGCAAGCATT
TAGATGATATTGACATTGATGAAATGTACGATGAATTAATGAGCAATCCGAAAGTGAAGAAGAAGCCACTAGATATTAGT
GCACGTGATATGCTTATTAAAATCGCTATGATTCAGCTTGAGTCTGGTTACCCATACTTAATGTTTAAATCAAATGCAAA
TAATCAACATCCATTGAAGGCTATTGGAACTGTGAAGATGTCGAACTTATGTACAGAAATCTTCCAGCTTCAAGAAACTT
CAGAAATAAATGATTACGGTACAGATGACATTATCCGTCGTGATATTAACTGTAACTTAGGATCGTTAAATATCGTAAAC
GTAATGGAAAATAAAGAAATTCGTGAAGCAGTTCATGCGGGAATGGAAGCTTTAACAGCTGTTTCTGATATGACGATTAT
TCCGAATGCACCAACTGTGAAAAAAGCAAATGACGAGCTTCATTCAGTTGGACTTGGAGCAATGAACTTACACGGATATT
TAGCAAAAAATAAAATCGCTTATGAAAGTTCAGAAGCGAAAGAATTCGCTCGTACATTCTTTATGATGTTAAATTACTAC
TCTATTGAGAAAAGTATGGAAATCGCTACAGAAAAAGGCGAGACATTTAAGGACTTCGATAAATCTGATTATGCGAATGG
CACATACTTCGAAAAGTATGAAACGACAGATTACAGCCCAGTAACTGAAAAAGTTCAGCAACTATTTGAAGGAATTCATA
TTCCAACAAAAGAAGATTGGACAAGTTTAAAAGAGCAAGTGCAGAAGAATGGTTTATACAACTCATATCGTCTTGCCATT
GCTCCAACACAATCAATCAGTTACGTTCAAAATGCAACTTCAAGCGTAATGCCGATCGTAAGTCAAATCGAATCAAGAAC
ATATGCGAATGCGACAACATATTATCCAATGCCGTATTTATCAAAAGATACGTTCTGGTACTATAAATCTTCTTACGATA
TGAATCAGTTTAAATTAATTGATTTAATCGCAGAAATTCAAGAACATATTGACCAAGGAATTAGTACAATTCTTTACGTT
AATAGTGATATTTCAACACGTGAATTAGCACGTTACTACATCTATGCACATAAAAAAGGCTTAAAGAGTCTGTATTATAC
GAGAACACGTAAATTAAGCGTGGAAGAGTGTGTGGCTTGTACCGTTTAA

Upstream 100 bases:

>100_bases
TCAAAATTTGAGTTATCTGGAACAAATAACGATGTAGAATATTTTAAGGAAAGGGTGCGGGAGATTGCGACACATTGAAC
TGAATAATGAAATCACGCAA

Downstream 100 bases:

>100_bases
TATAAAAAGTGAGACATGATGAGCAGGGTTTTCTGAACCCTGCTCATGCTTAGTTATCATATTTAGAGAAGGGGCGATTT
AATGCGTGCGGTAAACTGGA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MQDGFYQLHKDKEALEVFMEEARENTVPFNSVAERMEYMKEHDYYYNVLDEYSLEEVEEVYNIAYGENFEFQSYMAASKF
YKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVSKARQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLN
SIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
FLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDIS
ARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKAIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVN
VMENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESSEAKEFARTFFMMLNYY
SIEKSMEIATEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAI
APTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYV
NSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Sequences:

>Translated_682_residues
MQDGFYQLHKDKEALEVFMEEARENTVPFNSVAERMEYMKEHDYYYNVLDEYSLEEVEEVYNIAYGENFEFQSYMAASKF
YKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVSKARQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLN
SIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
FLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDIS
ARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKAIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVN
VMENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESSEAKEFARTFFMMLNYY
SIEKSMEIATEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAI
APTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYV
NSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
>Mature_682_residues
MQDGFYQLHKDKEALEVFMEEARENTVPFNSVAERMEYMKEHDYYYNVLDEYSLEEVEEVYNIAYGENFEFQSYMAASKF
YKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVSKARQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLN
SIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
FLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDIS
ARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKAIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVN
VMENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESSEAKEFARTFFMMLNYY
SIEKSMEIATEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAI
APTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYV
NSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=652, Percent_Identity=26.6871165644172, Blast_Score=216, Evalue=8e-56,
Organism=Escherichia coli, GI1789030, Length=689, Percent_Identity=44.267053701016, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI2367133, Length=557, Percent_Identity=23.1597845601436, Blast_Score=156, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17555242, Length=652, Percent_Identity=25.920245398773, Blast_Score=208, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6322123, Length=710, Percent_Identity=24.5070422535211, Blast_Score=206, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6320914, Length=658, Percent_Identity=24.9240121580547, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI17352481, Length=672, Percent_Identity=25.4464285714286, Blast_Score=199, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 78205; Mature: 78205

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDGFYQLHKDKEALEVFMEEARENTVPFNSVAERMEYMKEHDYYYNVLDEYSLEEVEEV
CCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
YNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVSKARQF
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHH
ASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGGVA
HHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCHHHCCCCCHHHHHHHHCCCCEE
LNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
EEHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
FLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDID
HHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHH
EMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKAIGTVKM
HHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHH
SNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVMENKEIREAVHAGMEALTA
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHH
VSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESSEAKEFARTFFMMLNYY
HHCCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHH
SIEKSMEIATEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDW
HHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHH
TSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYL
HHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
SKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYIYAHKKG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
LKSLYYTRTRKLSVEECVACTV
HHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MQDGFYQLHKDKEALEVFMEEARENTVPFNSVAERMEYMKEHDYYYNVLDEYSLEEVEEV
CCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
YNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVSKARQF
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHH
ASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGGVA
HHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCHHHCCCCCHHHHHHHHCCCCEE
LNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIE
EEHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
FLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDID
HHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHH
EMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKAIGTVKM
HHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHH
SNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVMENKEIREAVHAGMEALTA
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHH
VSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESSEAKEFARTFFMMLNYY
HHCCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHH
SIEKSMEIATEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDW
HHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHH
TSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYL
HHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
SKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYIYAHKKG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
LKSLYYTRTRKLSVEECVACTV
HHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]