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The map label for this gene is pphA [H]

Identifier: 218232403

GI number: 218232403

Start: 587164

End: 587868

Strand: Direct

Name: pphA [H]

Synonym: BCB4264_A0607

Alternate gene names: 218232403

Gene position: 587164-587868 (Clockwise)

Preceding gene: 218231651

Following gene: 218232940

Centisome position: 10.84

GC content: 35.32

Gene sequence:

>705_bases
ATGAAAAGAATTCTTGTTATTAGTGATATTCATGGAGAGATAGAAAAGTTCGAACAGTTATTGGAAGAAGCTCAATATGA
TGCGAAACAGGATCAATTAATCTTACTGGGAGATTATGTAGATCGTGGTCCAAATGCGCGTGCTGTAATTGAAAAGGTAA
GAGAATTGAAAAAAGAGGGAGCACTCATTTTAAAAGGAAATCATGAAGATATGATGATTAAGGCGTTAACGACAAATGAG
GAACGTTCATGGAACCATTGGGTAAAAAGAAATGGTGGAGATAAGACATTATATAGCTATGGATTTGTAGAAGAAGATAT
TGCGGTTAATGAGAAAGACTTCCAAAAACCGATTTTACACTCTCGTGTATTAGAGGAACATATAAAGTTTATTCAAGAAT
TAGATCATTACATTGAAACAGAAGAGTATATATTTGTACATGCTGGGGTTGAACCGATGAAACGAGTTTCTGAATCTGAA
CCATATACATTAATGTGGATCCGTAATGAATTTCATAATGGATATAGCGGTGAAAAGGTTGTTGTATTTGGGCATACGGA
AACAAAAACGCTTCATGGTAGTGAGGATTGCGGAGTGTATTTTGGTAGTAATCGTATTATTGGGATTGATGGCGGGGCTG
TGTATGGCGGTCAGCTAAATTGTTTAGAGTTGCCGAGTAAAAAGGTATATGTAATAAAAAATTGA

Upstream 100 bases:

>100_bases
TGAAACTGTAATCAGTGGGGGGCTTACTGCCCGTTAATGCGGGATTTCAATTCTGAACTTTCTGTCGTTTTGAACTATAA
TTAGAAAGAGGGGGGATAAT

Downstream 100 bases:

>100_bases
AAGAGATAAGAGGAATCTTATTTCTTTCAATTTTTTATTAAGCTAAATTTAAGACAAAGTTTTTATAATAAAACTTCAAA
ATTTTAGAAGGAAATAGTTA

Product: putative serine/threonine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVRELKKEGALILKGNHEDMMIKALTTNE
ERSWNHWVKRNGGDKTLYSYGFVEEDIAVNEKDFQKPILHSRVLEEHIKFIQELDHYIETEEYIFVHAGVEPMKRVSESE
PYTLMWIRNEFHNGYSGEKVVVFGHTETKTLHGSEDCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKN

Sequences:

>Translated_234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVRELKKEGALILKGNHEDMMIKALTTNE
ERSWNHWVKRNGGDKTLYSYGFVEEDIAVNEKDFQKPILHSRVLEEHIKFIQELDHYIETEEYIFVHAGVEPMKRVSESE
PYTLMWIRNEFHNGYSGEKVVVFGHTETKTLHGSEDCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKN
>Mature_234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVRELKKEGALILKGNHEDMMIKALTTNE
ERSWNHWVKRNGGDKTLYSYGFVEEDIAVNEKDFQKPILHSRVLEEHIKFIQELDHYIETEEYIFVHAGVEPMKRVSESE
PYTLMWIRNEFHNGYSGEKVVVFGHTETKTLHGSEDCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKN

Specific function: Can hydrolyze phosphorylated Ser-, Thr- or Tyr- substrates in vitro. The natural substrate is unknown [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=230, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 26959; Mature: 26959

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVRELKKEG
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
ALILKGNHEDMMIKALTTNEERSWNHWVKRNGGDKTLYSYGFVEEDIAVNEKDFQKPILH
CEEEECCCCCEEEEEEECCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
SRVLEEHIKFIQELDHYIETEEYIFVHAGVEPMKRVSESEPYTLMWIRNEFHNGYSGEKV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEE
VVFGHTETKTLHGSEDCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKN
EEEECCCCEEECCCCCCCEEECCCEEEEECCCEEECCEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVRELKKEG
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
ALILKGNHEDMMIKALTTNEERSWNHWVKRNGGDKTLYSYGFVEEDIAVNEKDFQKPILH
CEEEECCCCCEEEEEEECCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
SRVLEEHIKFIQELDHYIETEEYIFVHAGVEPMKRVSESEPYTLMWIRNEFHNGYSGEKV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCCCCCEE
VVFGHTETKTLHGSEDCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKN
EEEECCCCEEECCCCCCCEEECCCEEEEECCCEEECCEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11717262; 11677609 [H]