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The map label for this gene is nemA [H]

Identifier: 218232208

GI number: 218232208

Start: 3669149

End: 3670276

Strand: Reverse

Name: nemA [H]

Synonym: BCB4264_A3755

Alternate gene names: 218232208

Gene position: 3670276-3669149 (Counterclockwise)

Preceding gene: 218230882

Following gene: 218232873

Centisome position: 67.73

GC content: 40.25

Gene sequence:

>1128_bases
ATGACAAGTTCAAGTAATAAAGCAGCGAGTATTTATGAAGGAACAAATGTAAATGCTGGGGGATTTTTTGAAAACGTTGA
AGAAACAAAGTTGTTTGATCCAATTCAAGTTGGAGCTTGGTCTCTTCGTAATCGTATAGCGATGGCACCGATGACAAGAT
GCTTTGCAGATAATGAGACTGGAGTAGTTGGTGCGGATGTAGTGGAGTACTACAGAAAACGTGCTGCGGATGGAATTGGA
CTAATTATTACAGAGGGAATTGTCATTAGTCCGAGAGCAAAAGGAAACCCAGGAGTACCAGGTATTTATACACAAGAGCA
AATCGATTCTTGGAAACCTGTTACAGAGGCGGTACATAAAGAAGGTGGGACGATTATTGCTCAAATATGGCATGTTGGGC
GTATGAGTCATCATGAAATAATTGGTGGTCAAATGCCACAAGCACCGTCGGCTATCGCTGCAGAGGGAAATGTTCCACGC
TTTCGTAAACCGTTTGATACGCCAGAAGCAATGACGTTAGAAGAAATACAAGAAGTGATCGGGCAATACGCACAAGCTGC
GAAAAATGCGATCGAAGCTGGATTTGATGGAGTAGAAATTCACGGTGCACACGGATATTTAATCGATCAATTTACGTATG
AGTTTGCAAATAAGCGAATAGATAAATATGGCGGTGATTTAAAACAAAGGTTAACGTTTATGAAAGAAGTAACGGCGGCT
GTAATAGAAGCTGTTGGAGCTAATAAAACACTTCTTCGTTTCTCTGCTTTCAAAGGTGATAATCCTACTTATATGTGGGA
AAACCCTGAGTTTGCAATTGAAACGTTCATAAATATGTTTAAAGAGGTTGGGTTAACGATGATCCATCCTTCAACGATGA
ATTATACCCAAGAGATTGCTGACGGAAAGAATTTTCATCAACTAGTAAGAAAATATTGGGATGGTACGATTGTTGGGGTT
GGTAATTTAAATCCGAAAGAGGCCGAAGAAGCGTTGCAAGAAGGAACGATTGATGTAGCGGCATTCGGAAGACCGTTAAT
TGCTAATCCAGACTTTGTTCATCGAATTAAACACGCTGAAAGTTTAGTTGAATATGACGCGAAAGAGCATCTTGCTACGT
TAGTATGA

Upstream 100 bases:

>100_bases
TGGATATTCGAATTTTTTATTGCAAAGTCGTAAAAATTGACGTTACTATCTAAAAGTAAGTATGATGCGATTATAGTAAG
ATTTTTTAGGGGGAAGAAAA

Downstream 100 bases:

>100_bases
ATAAAGATAAAAGGAGAGCACATCTAGTGTTCTCCTTTTATTATGGAATTTCATAGGGGGAAGCGGGATGAGTGAAACGC
TACTCGTTATTATTAGTATA

Product: NADH-dependent flavin oxidoreductase, Oye family

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTSSSNKAASIYEGTNVNAGGFFENVEETKLFDPIQVGAWSLRNRIAMAPMTRCFADNETGVVGADVVEYYRKRAADGIG
LIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRIDKYGGDLKQRLTFMKEVTAA
VIEAVGANKTLLRFSAFKGDNPTYMWENPEFAIETFINMFKEVGLTMIHPSTMNYTQEIADGKNFHQLVRKYWDGTIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAESLVEYDAKEHLATLV

Sequences:

>Translated_375_residues
MTSSSNKAASIYEGTNVNAGGFFENVEETKLFDPIQVGAWSLRNRIAMAPMTRCFADNETGVVGADVVEYYRKRAADGIG
LIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRIDKYGGDLKQRLTFMKEVTAA
VIEAVGANKTLLRFSAFKGDNPTYMWENPEFAIETFINMFKEVGLTMIHPSTMNYTQEIADGKNFHQLVRKYWDGTIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAESLVEYDAKEHLATLV
>Mature_374_residues
TSSSNKAASIYEGTNVNAGGFFENVEETKLFDPIQVGAWSLRNRIAMAPMTRCFADNETGVVGADVVEYYRKRAADGIGL
IITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRF
RKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRIDKYGGDLKQRLTFMKEVTAAV
IEAVGANKTLLRFSAFKGDNPTYMWENPEFAIETFINMFKEVGLTMIHPSTMNYTQEIADGKNFHQLVRKYWDGTIVGVG
NLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAESLVEYDAKEHLATLV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=341, Percent_Identity=39.2961876832845, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=30.4347826086957, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17565138, Length=354, Percent_Identity=28.8135593220339, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=310, Percent_Identity=25.1612903225806, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=359, Percent_Identity=23.3983286908078, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=326, Percent_Identity=25.7668711656442, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=322, Percent_Identity=23.9130434782609, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=343, Percent_Identity=25.6559766763848, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=29.7674418604651, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=365, Percent_Identity=31.5068493150685, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321973, Length=359, Percent_Identity=31.1977715877437, Blast_Score=163, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41395; Mature: 41264

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSSNKAASIYEGTNVNAGGFFENVEETKLFDPIQVGAWSLRNRIAMAPMTRCFADNET
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVGADVVEYYRKRAADGIGLIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRIDKYGGDLKQRLTFMKEVTAA
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VIEAVGANKTLLRFSAFKGDNPTYMWENPEFAIETFINMFKEVGLTMIHPSTMNYTQEIA
HHHHHCCCCEEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHH
DGKNFHQLVRKYWDGTIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAE
CCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLV
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSSNKAASIYEGTNVNAGGFFENVEETKLFDPIQVGAWSLRNRIAMAPMTRCFADNET
CCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVGADVVEYYRKRAADGIGLIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRIDKYGGDLKQRLTFMKEVTAA
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VIEAVGANKTLLRFSAFKGDNPTYMWENPEFAIETFINMFKEVGLTMIHPSTMNYTQEIA
HHHHHCCCCEEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHH
DGKNFHQLVRKYWDGTIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAE
CCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLV
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]