Click here to switch to the map view.

The map label for this gene is yerD [H]

Identifier: 218232873

GI number: 218232873

Start: 3667507

End: 3669081

Strand: Reverse

Name: yerD [H]

Synonym: BCB4264_A3754

Alternate gene names: 218232873

Gene position: 3669081-3667507 (Counterclockwise)

Preceding gene: 218232208

Following gene: 218231033

Centisome position: 67.71

GC content: 38.22

Gene sequence:

>1575_bases
ATGAGTGAAACGCTACTCGTTATTATTAGTATATTACTATTTTTAATGTTGCTTTTAATCGTCTTTTTTATCATTACATT
TTTTATAAAAAAACGAACACACCATTCTATTTTAAAATTACATCCGTACTTAGGAAGAATGCGCTACTTACTGGAAAAGA
TAGGGCCTGAATTTCGACAATATTGGTTTGATCATGATACGGATGGAAAGCCTTTCTCACGTTATGATTTTCAAAGTGTT
ATGTTTTTAGCGAAATATCGCTCTGAAATACTTGGTTTTGGATCAAAACGAGATTTTGAAGCTTCTGGATATTATATTGC
TAATACATTATTTCCGATATTAACTGAGGAATTAAGTGTGAATCTCACGCAAGAGCGGGAAGGTAAAAAGTACGTGATTC
ATAAAGAAGGGTTATTTTCAAGAAGAGAAAAATTGACAGCGGATACAACGAATCTTTGGCTGTACGAAGAAGATGATGCA
ATTATAGTCGGTGAGAACCGTAAATATCCGTGGAAGCTACATGGCATGTTTGGTGCATCTGCGACTTCTTACGGTGCGAT
TGGAGAAAATTATATTTTAGCGAGTGGTTTTGGAGCAAAAATGGCAGGCGGATCGTGGATTAATACGGGTGAAGGTGGCG
TTATTCCAGAACATTTACATACAGGAGCGAATATCGTTGCGCAAATTGGTCCAGGGTTATTTGGATACCGTGATGAAGAC
GGTAATTTTTCAATGGGAAAATTCATGGAGAAAGCGAAAGAAAGTAATATTAGAGCATTCGAATTGAAATTTGGGCAAGG
TGCTAAAATACGTGGTGGTCATTTAGAGGGGCAAAAAGTAAATGAGAAAATTGCTTCTGTTCGCAACGTCCGAAAGGGAG
AGACGATTAATTCACCGAATCGATTTTCATTTTTAAAGAATGCAGCGGATACACTTTGTTTTATTCAACAGTTGCAAGAA
AACAGCGGTAAACCAGTTGGAATGAAAATTGTAATTGGTCAGCAGGAGCCTTTGGAAGATTTAATAAAAACAATGAAAGA
ACTAAACATTTACCCAGATTTTATTACAATCGACGGTTCAGAAGGCGGGTCAGGAGCAACGTATAAATCGATGGCAGATT
ATATGGGACTGCCGCTTATTCCAGCATTACTTACATTTATTGATACAGCAAACCATTATGGTGTCCGTAACAAGTTTAAA
GTATTTGCAGCGGGTAAATTAATCACACCAGATAAAGTGGCGATTGCTTTAGCGATTGGGGCGGATGCTGTAAGTTCAGC
CCGTGGGTTTATGATGGCAAGTGGCTGTATTATGGCCCTTCAATGTAATTCTGGTCAATGTCCATCTGGTGTAGCCACGA
CGAATCCGCATTATCAAAAAGCGTTAGATCCGTATGAGAAGAAGTGGCGAGTCATGAATTATATTATTAGTATGAGATAT
AGTTTATTTTCATTAGCGGCAGCGGCGGGAGTGAAGAGCCCGCGCCATTTAACTAGAGAGCATATTGTGTTTAAAGACGA
GGTGGGGAGAGTAGTTCCTCTTTCGGAATTGTTTCCTATAGTAAATCAAACCTAA

Upstream 100 bases:

>100_bases
GACGCGAAAGAGCATCTTGCTACGTTAGTATGAATAAAGATAAAAGGAGAGCACATCTAGTGTTCTCCTTTTATTATGGA
ATTTCATAGGGGGAAGCGGG

Downstream 100 bases:

>100_bases
AAGTATATTTGATGTGTAAGATTTTTTAGAGGAATAATTATATTTTTGGAAAAATTAGGAGTATAATAGGGGAGTATTTT
TGAAAATGACTATTTTTAGG

Product: ferredoxin-dependent glutamate synthase

Products: L-glutamine; 2-oxoglutarate; reduced ferredoxin

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSETLLVIISILLFLMLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQYWFDHDTDGKPFSRYDFQSV
MFLAKYRSEILGFGSKRDFEASGYYIANTLFPILTEELSVNLTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDA
IIVGENRKYPWKLHGMFGASATSYGAIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
GNFSMGKFMEKAKESNIRAFELKFGQGAKIRGGHLEGQKVNEKIASVRNVRKGETINSPNRFSFLKNAADTLCFIQQLQE
NSGKPVGMKIVIGQQEPLEDLIKTMKELNIYPDFITIDGSEGGSGATYKSMADYMGLPLIPALLTFIDTANHYGVRNKFK
VFAAGKLITPDKVAIALAIGADAVSSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
SLFSLAAAAGVKSPRHLTREHIVFKDEVGRVVPLSELFPIVNQT

Sequences:

>Translated_524_residues
MSETLLVIISILLFLMLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQYWFDHDTDGKPFSRYDFQSV
MFLAKYRSEILGFGSKRDFEASGYYIANTLFPILTEELSVNLTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDA
IIVGENRKYPWKLHGMFGASATSYGAIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
GNFSMGKFMEKAKESNIRAFELKFGQGAKIRGGHLEGQKVNEKIASVRNVRKGETINSPNRFSFLKNAADTLCFIQQLQE
NSGKPVGMKIVIGQQEPLEDLIKTMKELNIYPDFITIDGSEGGSGATYKSMADYMGLPLIPALLTFIDTANHYGVRNKFK
VFAAGKLITPDKVAIALAIGADAVSSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
SLFSLAAAAGVKSPRHLTREHIVFKDEVGRVVPLSELFPIVNQT
>Mature_523_residues
SETLLVIISILLFLMLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQYWFDHDTDGKPFSRYDFQSVM
FLAKYRSEILGFGSKRDFEASGYYIANTLFPILTEELSVNLTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDAI
IVGENRKYPWKLHGMFGASATSYGAIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDEDG
NFSMGKFMEKAKESNIRAFELKFGQGAKIRGGHLEGQKVNEKIASVRNVRKGETINSPNRFSFLKNAADTLCFIQQLQEN
SGKPVGMKIVIGQQEPLEDLIKTMKELNIYPDFITIDGSEGGSGATYKSMADYMGLPLIPALLTFIDTANHYGVRNKFKV
FAAGKLITPDKVAIALAIGADAVSSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRYS
LFSLAAAAGVKSPRHLTREHIVFKDEVGRVVPLSELFPIVNQT

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI308199519, Length=265, Percent_Identity=30.5660377358491, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17570289, Length=322, Percent_Identity=27.0186335403727, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=364, Percent_Identity=24.1758241758242, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28574881, Length=344, Percent_Identity=25, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665539, Length=344, Percent_Identity=25, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: 1.4.7.1

Molecular weight: Translated: 58571; Mature: 58440

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLLVIISILLFLMLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
YWFDHDTDGKPFSRYDFQSVMFLAKYRSEILGFGSKRDFEASGYYIANTLFPILTEELSV
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCC
NLTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDAIIVGENRKYPWKLHGMFGAS
CEEECCCCCEEEEECCCHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCC
ATSYGAIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
CCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHCCHHHHHHHCCCCCCEECCC
GNFSMGKFMEKAKESNIRAFELKFGQGAKIRGGHLEGQKVNEKIASVRNVRKGETINSPN
CCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
RFSFLKNAADTLCFIQQLQENSGKPVGMKIVIGQQEPLEDLIKTMKELNIYPDFITIDGS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECC
EGGSGATYKSMADYMGLPLIPALLTFIDTANHYGVRNKFKVFAAGKLITPDKVAIALAIG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEC
ADAVSSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
CHHHHHHCCCEEECCEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
SLFSLAAAAGVKSPRHLTREHIVFKDEVGRVVPLSELFPIVNQT
HHHHHHHHHCCCCCHHHHHHHEEEHHHCCCEECHHHHHHHHCCC
>Mature Secondary Structure 
SETLLVIISILLFLMLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
YWFDHDTDGKPFSRYDFQSVMFLAKYRSEILGFGSKRDFEASGYYIANTLFPILTEELSV
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCC
NLTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDAIIVGENRKYPWKLHGMFGAS
CEEECCCCCEEEEECCCHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCC
ATSYGAIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
CCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHCCHHHHHHHCCCCCCEECCC
GNFSMGKFMEKAKESNIRAFELKFGQGAKIRGGHLEGQKVNEKIASVRNVRKGETINSPN
CCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
RFSFLKNAADTLCFIQQLQENSGKPVGMKIVIGQQEPLEDLIKTMKELNIYPDFITIDGS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECC
EGGSGATYKSMADYMGLPLIPALLTFIDTANHYGVRNKFKVFAAGKLITPDKVAIALAIG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEC
ADAVSSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
CHHHHHHCCCEEECCEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
SLFSLAAAAGVKSPRHLTREHIVFKDEVGRVVPLSELFPIVNQT
HHHHHHHHHCCCCCHHHHHHHEEEHHHCCCEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; oxidized ferredoxin

Specific reaction: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377; 8969499 [H]