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The map label for this gene is dacA [H]

Identifier: 218232010

GI number: 218232010

Start: 2442781

End: 2443797

Strand: Reverse

Name: dacA [H]

Synonym: BCB4264_A2523

Alternate gene names: 218232010

Gene position: 2443797-2442781 (Counterclockwise)

Preceding gene: 218231270

Following gene: 218232469

Centisome position: 45.1

GC content: 30.09

Gene sequence:

>1017_bases
ATGCAATTCTTAAAGAAATTCGCTATTTTATTATTATCTTTTTTTATTACTGCACTTATCGTTTTATATTTTTATCTTTA
TGTAAATGGTCCGATTATTCAAGCTAAATCAGCTATTTTAATAAATGCTAATTCAGGTGAAATTGTTTATAAAAAAAATG
AAGAAACTCCAGTACAATCAGCTGCTTTATCTAAATTAATGACAGAATATATCGTATTGGAGCAACTAAATGATAGAAAA
ATACAGTTAGATGACTTAGTACAAATAAGTAACGAAGTTTTCCGAGTAGAAACAAGCCCTATACAAGTAACATCGAATGA
TAAAACAACGGTTCGTGATTTACTTCATGCATTGCTACTGGCAGGGAATAATCGTTCTGCACTCGCCCTAGCAGAACACA
TTGCTGGAAATGAAGATAACTTTACAGTATTAATGAATAAAAAAGCAAAAGAACTAAAGTTATTACAACCATCTCCATTT
CTAAACTCTACTGGAATTAATAATAATACAAATAAACAATCAACTACAACAGCGATAGACGCAGCTAAACTAGCAGCACG
ATTAGTAAAAGATTTTCCAGATGTATTGAATATTACGAAACTAACCTCTTACCAATTTACCTTCAAAGATTCCCAGGTTT
TCAATACGAATAAAATGATATATTCCCTTAATGAAAACATTAAACTTCAAGGTGTTGATGGCTTACAAACTAGTTTTTCA
ACTAACGGTAATTATAGTTTTGTCAGTACAGCAAAACTTGGAGATACTAGACTAATTTCAGTAATATTAGATGCAGATGA
AGAAAACATTTCATTTATTGAAACAAAAAAGTTGCTCCAGTATGGTTTTGATCCTTCCTCGTACTCTGCACTCCAAGCTT
TTAAAGACGCCCTTACATCTTGGACAATATTGCTTCAGTTTAAAAATTTAATTATACAAACTATAATGATTTTTTTAATC
ATTACCACCTTAATGTTTTTACATATACGTCAGAAAAAATCCGAGGATTTCAATTAA

Upstream 100 bases:

>100_bases
TCCTTTATAATTCTTTAGAAATTCTTTACCTTTTCTTTCTTCCTAATTAAGCATTCCTTTTTACAATACTATTATTGATG
TCATAATAGGAGGATCGCTT

Downstream 100 bases:

>100_bases
GAAATCTTCTTATATATATAAAACCGACTTGCGCTTGAGATGCAAGTCGGTTTTTAATTCTATCATCCTCTTCCAAGCAA
ATTTGCTGAAACTGGTTGCA

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINANSGEIVYKKNEETPVQSAALSKLMTEYIVLEQLNDRK
IQLDDLVQISNEVFRVETSPIQVTSNDKTTVRDLLHALLLAGNNRSALALAEHIAGNEDNFTVLMNKKAKELKLLQPSPF
LNSTGINNNTNKQSTTTAIDAAKLAARLVKDFPDVLNITKLTSYQFTFKDSQVFNTNKMIYSLNENIKLQGVDGLQTSFS
TNGNYSFVSTAKLGDTRLISVILDADEENISFIETKKLLQYGFDPSSYSALQAFKDALTSWTILLQFKNLIIQTIMIFLI
ITTLMFLHIRQKKSEDFN

Sequences:

>Translated_338_residues
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINANSGEIVYKKNEETPVQSAALSKLMTEYIVLEQLNDRK
IQLDDLVQISNEVFRVETSPIQVTSNDKTTVRDLLHALLLAGNNRSALALAEHIAGNEDNFTVLMNKKAKELKLLQPSPF
LNSTGINNNTNKQSTTTAIDAAKLAARLVKDFPDVLNITKLTSYQFTFKDSQVFNTNKMIYSLNENIKLQGVDGLQTSFS
TNGNYSFVSTAKLGDTRLISVILDADEENISFIETKKLLQYGFDPSSYSALQAFKDALTSWTILLQFKNLIIQTIMIFLI
ITTLMFLHIRQKKSEDFN
>Mature_338_residues
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINANSGEIVYKKNEETPVQSAALSKLMTEYIVLEQLNDRK
IQLDDLVQISNEVFRVETSPIQVTSNDKTTVRDLLHALLLAGNNRSALALAEHIAGNEDNFTVLMNKKAKELKLLQPSPF
LNSTGINNNTNKQSTTTAIDAAKLAARLVKDFPDVLNITKLTSYQFTFKDSQVFNTNKMIYSLNENIKLQGVDGLQTSFS
TNGNYSFVSTAKLGDTRLISVILDADEENISFIETKKLLQYGFDPSSYSALQAFKDALTSWTILLQFKNLIIQTIMIFLI
ITTLMFLHIRQKKSEDFN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=262, Percent_Identity=32.0610687022901, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87082022, Length=259, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786851, Length=261, Percent_Identity=29.5019157088123, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082054, Length=256, Percent_Identity=25, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 38067; Mature: 38067

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINANSGEIVYKKNEETPVQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCEEEEECCCCCCHHH
AALSKLMTEYIVLEQLNDRKIQLDDLVQISNEVFRVETSPIQVTSNDKTTVRDLLHALLL
HHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHH
AGNNRSALALAEHIAGNEDNFTVLMNKKAKELKLLQPSPFLNSTGINNNTNKQSTTTAID
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHH
AAKLAARLVKDFPDVLNITKLTSYQFTFKDSQVFNTNKMIYSLNENIKLQGVDGLQTSFS
HHHHHHHHHHHCHHHHHHHHHEEEEEEECCCEEECCCCEEEEECCCEEEEECCCCHHCCC
TNGNYSFVSTAKLGDTRLISVILDADEENISFIETKKLLQYGFDPSSYSALQAFKDALTS
CCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WTILLQFKNLIIQTIMIFLIITTLMFLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINANSGEIVYKKNEETPVQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCEEEEECCCCCCHHH
AALSKLMTEYIVLEQLNDRKIQLDDLVQISNEVFRVETSPIQVTSNDKTTVRDLLHALLL
HHHHHHHHHHHHHHHCCCCEECHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHH
AGNNRSALALAEHIAGNEDNFTVLMNKKAKELKLLQPSPFLNSTGINNNTNKQSTTTAID
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHH
AAKLAARLVKDFPDVLNITKLTSYQFTFKDSQVFNTNKMIYSLNENIKLQGVDGLQTSFS
HHHHHHHHHHHCHHHHHHHHHEEEEEEECCCEEECCCCEEEEECCCEEEEECCCCHHCCC
TNGNYSFVSTAKLGDTRLISVILDADEENISFIETKKLLQYGFDPSSYSALQAFKDALTS
CCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WTILLQFKNLIIQTIMIFLIITTLMFLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]