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The map label for this gene is vanS [H]

Identifier: 218231270

GI number: 218231270

Start: 2443973

End: 2445130

Strand: Reverse

Name: vanS [H]

Synonym: BCB4264_A2525

Alternate gene names: 218231270

Gene position: 2445130-2443973 (Counterclockwise)

Preceding gene: 218236069

Following gene: 218232010

Centisome position: 45.12

GC content: 30.92

Gene sequence:

>1158_bases
TTGAGAAATGAAGCATTTGATATATTAAAAGATATTCCTAAATGGAAACTTATTTTTTGGCTTTTATTAGCTATCACACT
CACTCCTATTACTGAATTAATTATTTATCGCCTCGTTACGAGTGTAATTGAAAGTTCACTTACTCTAACCGAACTCGGTA
AAGAATTCATTCGAATTTTTGGTTATGAAAAGTATAAAGGGATTAAAGAGTCTTTATGGATGATGATTGTATCTTATTTG
TTTTTATTTTCTATTTTTTCATCCTATCTTTACATTTTTTATCGTCACGAGAAAAAACTTTATTATGAAACTTGTATAAA
AAAAATGATAGAAGAAATTCGTTATATTGCAAATGGAAACTTCAATCATAAAGTTTCTATTGTAAATCATAATTATCTAG
AAGAATTAGCAATCGGGGTCAATGAAATTGTTGAACAATTAAAAGTCTCCATTGATGAAGAAAGACAAGCTGAACAAGCA
AAAAGTGAACTTATTACAAATGTATCACATGACTTACGCACTCCCCTTACCTCTATAGTTGGATACGTAAATTTAATCCA
TCATGATAATTATAGAGATGAAGTGGAATTGCGATACTATATTCAAGTCATTTACGATAAGGTAACTCGTCTTAATGCAT
TAATGAATGACTTGTTCGAATATACACGTGTTCAAAATAAAGAATTAAGCTTGTATTCTGTTCCTATTGATATTGTAGAG
TTACTTGGACAATTAACTGTTCAATTCCGTATACAATTTCAAGAAGCTAATATAGAATGTCGACCTTCTTTCCCGTCTCA
AAAGCTCATGGTATTAGCTGATGGTGACAAATTAGTGCGTGTGTTTGAAAATCTTATTATAAACGCTATCGCTTATGGAA
GCGAAGGTCGCTATATTGACCTTACTGCTTATGAAAGCAATAATATGATTACAATTGATATAACAAATTACGGACAACCT
ATTCCTAGTACGGATTTACCTCATATTTTTGAACGTTTTTATCGCGTTGAGAAATCTCGCTCTACAAACACTGGTGGATC
TGGACTTGGATTAGCAATTGCAAAAAGTATAGTCGAATTGCATAAAGGAACAATTGAAGTATATAGTGATGACGAAAAAA
CAACATTCACTGTAAAACTACTCCCATACAAATGTTAA

Upstream 100 bases:

>100_bases
AAGACGGTAATGGTCCATATTAGCAACTTACGAGATAAACTTGAAACAGTAATAAATGGAGAAAAATTTATTCATACTGT
TTGGGGAGTTGGCTATAAAA

Downstream 100 bases:

>100_bases
TATTACAAATAAAAAGTTCAATTTCTTACCATATAAGAAATTGAACTTTTTCCATAATTACCCTAAATTTAATAATCCTT
TATAATTCTTTAGAAATTCT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLTELGKEFIRIFGYEKYKGIKESLWMMIVSYL
FLFSIFSSYLYIFYRHEKKLYYETCIKKMIEEIRYIANGNFNHKVSIVNHNYLEELAIGVNEIVEQLKVSIDEERQAEQA
KSELITNVSHDLRTPLTSIVGYVNLIHHDNYRDEVELRYYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
LLGQLTVQFRIQFQEANIECRPSFPSQKLMVLADGDKLVRVFENLIINAIAYGSEGRYIDLTAYESNNMITIDITNYGQP
IPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVELHKGTIEVYSDDEKTTFTVKLLPYKC

Sequences:

>Translated_385_residues
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLTELGKEFIRIFGYEKYKGIKESLWMMIVSYL
FLFSIFSSYLYIFYRHEKKLYYETCIKKMIEEIRYIANGNFNHKVSIVNHNYLEELAIGVNEIVEQLKVSIDEERQAEQA
KSELITNVSHDLRTPLTSIVGYVNLIHHDNYRDEVELRYYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
LLGQLTVQFRIQFQEANIECRPSFPSQKLMVLADGDKLVRVFENLIINAIAYGSEGRYIDLTAYESNNMITIDITNYGQP
IPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVELHKGTIEVYSDDEKTTFTVKLLPYKC
>Mature_385_residues
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLTELGKEFIRIFGYEKYKGIKESLWMMIVSYL
FLFSIFSSYLYIFYRHEKKLYYETCIKKMIEEIRYIANGNFNHKVSIVNHNYLEELAIGVNEIVEQLKVSIDEERQAEQA
KSELITNVSHDLRTPLTSIVGYVNLIHHDNYRDEVELRYYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
LLGQLTVQFRIQFQEANIECRPSFPSQKLMVLADGDKLVRVFENLIINAIAYGSEGRYIDLTAYESNNMITIDITNYGQP
IPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVELHKGTIEVYSDDEKTTFTVKLLPYKC

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=27.8985507246377, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786783, Length=248, Percent_Identity=29.0322580645161, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI48994928, Length=247, Percent_Identity=27.5303643724696, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=28.2608695652174, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786912, Length=274, Percent_Identity=26.6423357664234, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=32.2033898305085, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693157, Length=311, Percent_Identity=23.7942122186495, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789149, Length=293, Percent_Identity=25.938566552901, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790346, Length=264, Percent_Identity=25.3787878787879, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=282, Percent_Identity=26.241134751773, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788549, Length=264, Percent_Identity=29.1666666666667, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787894, Length=261, Percent_Identity=26.0536398467433, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=23.9837398373984, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082128, Length=223, Percent_Identity=27.3542600896861, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788279, Length=227, Percent_Identity=26.8722466960352, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=224, Percent_Identity=25.4464285714286, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789808, Length=228, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44885; Mature: 44885

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLTELGKEFIRIF
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GYEKYKGIKESLWMMIVSYLFLFSIFSSYLYIFYRHEKKLYYETCIKKMIEEIRYIANGN
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCHHHHHHHHHHHHHHHHHHHHCCC
FNHKVSIVNHNYLEELAIGVNEIVEQLKVSIDEERQAEQAKSELITNVSHDLRTPLTSIV
CCEEEEEECHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVNLIHHDNYRDEVELRYYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHH
LLGQLTVQFRIQFQEANIECRPSFPSQKLMVLADGDKLVRVFENLIINAIAYGSEGRYID
HHHHHEEEEEEEEEECCEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHEECCCCCEEE
LTAYESNNMITIDITNYGQPIPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVEL
EEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
HKGTIEVYSDDEKTTFTVKLLPYKC
HCCEEEEEECCCCEEEEEEEECCCC
>Mature Secondary Structure
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLTELGKEFIRIF
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GYEKYKGIKESLWMMIVSYLFLFSIFSSYLYIFYRHEKKLYYETCIKKMIEEIRYIANGN
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCHHHHHHHHHHHHHHHHHHHHCCC
FNHKVSIVNHNYLEELAIGVNEIVEQLKVSIDEERQAEQAKSELITNVSHDLRTPLTSIV
CCEEEEEECHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVNLIHHDNYRDEVELRYYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHH
LLGQLTVQFRIQFQEANIECRPSFPSQKLMVLADGDKLVRVFENLIINAIAYGSEGRYID
HHHHHEEEEEEEEEECCEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHEECCCCCEEE
LTAYESNNMITIDITNYGQPIPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVEL
EEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
HKGTIEVYSDDEKTTFTVKLLPYKC
HCCEEEEEECCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]