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The map label for this gene is holB [H]

Identifier: 218231662

GI number: 218231662

Start: 36590

End: 37573

Strand: Direct

Name: holB [H]

Synonym: BCB4264_A0036

Alternate gene names: 218231662

Gene position: 36590-37573 (Clockwise)

Preceding gene: 218231073

Following gene: 218235276

Centisome position: 0.68

GC content: 36.69

Gene sequence:

>984_bases
ATGACGAAGACGTGGGAGCAGCTTTCTGCTATACAACCCATCGGTGTAAAAATGTTGATGAATAGCATTGCAAAAGAGCG
TATATCCCACGCTTACTTGTTAGAGGGAGGGAAAGGGACAGGAAAGTTTGCGACAGCAATTCAAATGGCAAAGAGTTTCC
TCTGTTCTGAGCGGAATGGAGTAGAGCCCTGCCATGTATGTACAAACTGTAAGCGAATTGATTCAGGTAACCACCCTAAC
TTACATATTGTAAAACCAGACGGATTATCTATTAAGAAGCAGCAAATTCATGATTTACAAGAAGAGTTTTCCAAAACAGG
ATTAGAGGCAAATAAAAAAATATATATTATTGAGCACGCAGATCGTATGACAGCAAATGCGGCAAATACACTTTTGAAAT
TTTTAGAAGAACCAAGTAGTGATACAACTGCTATTTTACTTACTGAACAAAGTCATCAAATTTTAAATACGATTTTATCT
CGCTGCCAAGTTGTTACGTTTAGACCGTTACCTACGGAATCTTTAATTAGAAGATTACAGGATGAAGGGATTACAGCATC
TTTATCGACACTTGCTGCTCAACTTACAAATAGTTTTGATGAAGCTTTAGCTTTATGTAGTGATGAATGGTTTGCACAAG
CACGAGCTTTAGTGATAAAATTATGTGAAGCGCTCGAAAAAGATAAAACCTCTATTTTTTTTGTACAAGAAAAATGGGGA
AAACACTTTGGAGAGAAAGAACAATTACAGCAAGGTTTAGATATGTTACTCCTTATTTATAAGGATTTATTATATGTTCA
ACTTGGAGAAGAAGATCGTCTTGTTTTTCATGAGCAGAAAGAGATGTTTGAATCGTTTTCCTATGCTCAGAAGCGCATTG
TATCAGCTCTCTTTAATATATTAGAGGCAAAAAATAGAATCAACGCTAATGTAAATGCGCAGCTTGTGTTCGAACAGTTA
GTGTTGCGGTTGCAGGAGGGATGA

Upstream 100 bases:

>100_bases
CTGATCAACCGATGGAAAAACTTATAGAAGAAGTTGTTCAGATTATAGAAGATAAATTGTTATAATAGAAGGAAAGAATG
AAATAAGTGAGGTATGCATT

Downstream 100 bases:

>100_bases
CCGTTTTGTATGATGTAGTAGGTGTTCGCTTTAAGAAGGCCGGAAAGGTATATTACTTTGATCCAAACCAGTTCGATATC
TCTGAAAATGAGTTTGTCAT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGGKGTGKFATAIQMAKSFLCSERNGVEPCHVCTNCKRIDSGNHPN
LHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKIYIIEHADRMTANAANTLLKFLEEPSSDTTAILLTEQSHQILNTILS
RCQVVTFRPLPTESLIRRLQDEGITASLSTLAAQLTNSFDEALALCSDEWFAQARALVIKLCEALEKDKTSIFFVQEKWG
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFHEQKEMFESFSYAQKRIVSALFNILEAKNRINANVNAQLVFEQL
VLRLQEG

Sequences:

>Translated_327_residues
MTKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGGKGTGKFATAIQMAKSFLCSERNGVEPCHVCTNCKRIDSGNHPN
LHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKIYIIEHADRMTANAANTLLKFLEEPSSDTTAILLTEQSHQILNTILS
RCQVVTFRPLPTESLIRRLQDEGITASLSTLAAQLTNSFDEALALCSDEWFAQARALVIKLCEALEKDKTSIFFVQEKWG
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFHEQKEMFESFSYAQKRIVSALFNILEAKNRINANVNAQLVFEQL
VLRLQEG
>Mature_326_residues
TKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGGKGTGKFATAIQMAKSFLCSERNGVEPCHVCTNCKRIDSGNHPNL
HIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKIYIIEHADRMTANAANTLLKFLEEPSSDTTAILLTEQSHQILNTILSR
CQVVTFRPLPTESLIRRLQDEGITASLSTLAAQLTNSFDEALALCSDEWFAQARALVIKLCEALEKDKTSIFFVQEKWGK
HFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFHEQKEMFESFSYAQKRIVSALFNILEAKNRINANVNAQLVFEQLV
LRLQEG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=145, Percent_Identity=33.7931034482759, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787341, Length=219, Percent_Identity=28.7671232876712, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37013; Mature: 36882

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGGKGTGKFATAIQMAKSFLCSERNG
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCC
VEPCHVCTNCKRIDSGNHPNLHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKIYIIEHA
CCHHHHHCCCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
DRMTANAANTLLKFLEEPSSDTTAILLTEQSHQILNTILSRCQVVTFRPLPTESLIRRLQ
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCEEEEECCCCHHHHHHHHH
DEGITASLSTLAAQLTNSFDEALALCSDEWFAQARALVIKLCEALEKDKTSIFFVQEKWG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHC
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFHEQKEMFESFSYAQKRIVSALFNI
CCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAKNRINANVNAQLVFEQLVLRLQEG
HHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKTWEQLSAIQPIGVKMLMNSIAKERISHAYLLEGGKGTGKFATAIQMAKSFLCSERNG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCC
VEPCHVCTNCKRIDSGNHPNLHIVKPDGLSIKKQQIHDLQEEFSKTGLEANKKIYIIEHA
CCHHHHHCCCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
DRMTANAANTLLKFLEEPSSDTTAILLTEQSHQILNTILSRCQVVTFRPLPTESLIRRLQ
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCEEEEECCCCHHHHHHHHH
DEGITASLSTLAAQLTNSFDEALALCSDEWFAQARALVIKLCEALEKDKTSIFFVQEKWG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHC
KHFGEKEQLQQGLDMLLLIYKDLLYVQLGEEDRLVFHEQKEMFESFSYAQKRIVSALFNI
CCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAKNRINANVNAQLVFEQLVLRLQEG
HHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]