Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 218231618

GI number: 218231618

Start: 319013

End: 320548

Strand: Direct

Name: purH [H]

Synonym: BCB4264_A0344

Alternate gene names: 218231618

Gene position: 319013-320548 (Clockwise)

Preceding gene: 218232251

Following gene: 218233779

Centisome position: 5.89

GC content: 37.96

Gene sequence:

>1536_bases
ATGAAAAAGCGTGCATTAGTAAGTGTTTCAGATAAAACAGGAGTAGTAGAATTTGTTAAAGGGTTACTAGAACAAGGGAT
TGAAGTTATTTCAACAGGTGGTACGAAAAAGTTACTAGAAGAAAATGGCTTACAAGTAATCGGCATTTCTGAAGTAACTG
GTTTCCCTGAAATTATGGATGGCCGTGTGAAAACATTACATCCAAATATTCATGGTGGTCTATTAGCAGTTCGTGATAAT
GAAACACATGTAACACAAATGAATGAGTTAGGTATAGAGCCAATTGACTTTGTTGTTGTTAACTTATACCCATTTAAAGA
AACAATCGCTAAGCCTGATGTAACATTTGCTGATGCGATTGAAAATATTGATATCGGTGGCCCAACAATGATTCGCTCTG
CTGCGAAAAATCATAAATTCGTATCGGTAATTGTAGATCCAGTAGATTATGATATTGTGTTAGCAGAACTGAAAGAGAAC
GGTGAAGTAGCAGAGGAAACAAAACGTAAACTAGCAGCGAAAGTATTCCGTCATACAGCAGCGTATGATGCGTTAATTTC
TAACTACTTAACAGAGCAAATGGGTGAAGAAAGTCCAGAAACATTAACTGTGACATTTGAGAAAAAGCAAGACTTACGCT
ATGGCGAGAACCCACATCAAAAAGCAACTTTCTATAAAGCGCCATTCGCAGCAACGTCTTCTGTTGCATACGCAGAACAA
TTACACGGTAAGGAATTATCGTATAACAATATTAATGATGCAGACGCAGCGCTTAGCATCGTAAAAGAATTTACAGAACC
AGCAGTAGTAGCAGTAAAGCATATGAATCCATGTGGTGTTGGAGTAGGAACTAATATTCACGAAGCATATACTCGTGCTT
ATGAAGCAGATCCAGTATCAATCTTCGGCGGTATTATTGCAGCAAATCGTGAAATTGATAAAGCTACAGCAGAAAAGTTA
CATGAAATTTTCTTAGAAATTGTTATCGCACCTTCGTTCTCGCAAGAAGCTTTAGAAGTGTTGCAAAGTAAGAAGAACTT
ACGTTTATTAACTATAAATATTGAAAAAGCGACAAGTGCAAGCAAAAAACTAACTTCTGTACAAGGTGGACTTCTCGTTC
AAGAGGAAGATACGTTATCATTAGATGAAAGTACAATTTCAATTCCAACGAAACGTGAACCTTCAGAGCAAGAATGGAAA
GATTTAAAACTAGCTTGGAAAGTTGTAAAACATGTGAAATCAAACGCAATTGTTTTAGCGAAAGATGACATGACAATTGG
TGTCGGTGCGGGACAGATGAACCGTGTAGGTTCTGCAAAAATCGCAATTACACAAGCTGGTGAAAAAGCACAAGGTAGCG
CGCTTGCATCTGATGCTTTCTTCCCAATGCCAGATACATTAGAAGAAGCAGCAAAAGCAGGAATTACAGCAATCATTCAA
CCGGGCGGATCAATCCGTGATGAAGATTCTATTAAAGTGGCAGATACTTATGGGATTGCTATGGTGTTCACTGGCGTACG
TCATTTCAAACACTAA

Upstream 100 bases:

>100_bases
ACAAGTTGAACATAAATTATACGTAAATACAGTGAATCAAATTGTTCAGTCTGTGAAAGAATCAACTGTTAACTAACATA
CAACCAGGGGTGAAGGATAA

Downstream 100 bases:

>100_bases
TAGAGGATGTTTAAAAAGTCCGGTTAAGATAACCTTCGCATGTCGTCGTTGCATCGCCAGTGCGGTACTCATGTAGGAAA
GCTACACTCCGTGTCCTCCT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVTQMNELGIEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKEN
GEVAEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTNIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIVIAPSFSQEALEVLQSKKNLRLLTINIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH

Sequences:

>Translated_511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVTQMNELGIEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKEN
GEVAEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTNIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIVIAPSFSQEALEVLQSKKNLRLLTINIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
>Mature_511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVTQMNELGIEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKEN
GEVAEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTNIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIVIAPSFSQEALEVLQSKKNLRLLTINIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=36.7983367983368, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=53.0534351145038, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71985564, Length=484, Percent_Identity=34.7107438016529, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=312, Percent_Identity=27.5641025641026, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=36.3829787234043, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=478, Percent_Identity=35.7740585774059, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24649832, Length=481, Percent_Identity=38.045738045738, Blast_Score=290, Evalue=2e-78,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55556; Mature: 55556

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNETHVTQMNELGIEPIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCCCHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKENGEVAEETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTNIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHH
IFGGIIAANREIDKATAEKLHEIFLEIVIAPSFSQEALEVLQSKKNLRLLTINIEKATSA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEHHHCCH
SKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWKDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNETHVTQMNELGIEPIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCCCHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKENGEVAEETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAATSSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTNIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHH
IFGGIIAANREIDKATAEKLHEIFLEIVIAPSFSQEALEVLQSKKNLRLLTINIEKATSA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEHHHCCH
SKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWKDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA