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The map label for this gene is purD [H]

Identifier: 218233779

GI number: 218233779

Start: 320814

End: 322085

Strand: Direct

Name: purD [H]

Synonym: BCB4264_A0345

Alternate gene names: 218233779

Gene position: 320814-322085 (Clockwise)

Preceding gene: 218231618

Following gene: 218236036

Centisome position: 5.92

GC content: 38.21

Gene sequence:

>1272_bases
ATGAATGTTTTAGTAATTGGCCGCGGTGGGCGTGAGCATGCTTTAGCTTGGAAGTTTGCACAATCTGAAAAAGTAGAAAA
GGTATATGTAGCACCAGGTAATGAAGGTATGAGAGATGTTGCAACACCAGTTGATATTGATGAAAATGATTTTGATGCAT
TAGTTTTATTTGCAAAAGAGAATAATGTTGAGTTAACTTTCGTTGGACCAGAAATTCCGCTTATGAATGGAATTGTTGAT
CGTTTTAAAGAAGAGGGACTTCGCGTATTTGGTCCGAATAAAGCGGCTGCTGTTATTGAAGGTAGTAAAGCTTTTACAAA
AGAGCTTATGAAAAAGTATGATATTCCAACTGCGGCGTACGAAACTTTTACAGATTATGAAGAAGCAGTACAGTACATTC
AAAAAGTTGGTGCACCAATCGTTATTAAAGCTGACGGCCTAGCTGCTGGTAAAGGTGTAACGGTAGCGATGTCGCTTGAA
GAGGCATTACAAGCTGTGAAAGAAATGCTGCAAGATGTGAAATTCGGCGCGGCAAGTAAGAAGGTTGTTATTGAAGAGTT
TTTAGATGGACAAGAATTTTCATTAATGGCATTCGTGAATGGAACAACTGTACATCCAATGGTAATCGCCCAAGATCATA
AAAGAGCTTTTGATGGTGATAAAGGTCCAAATACGGGTGGAATGGGTGCATATTCTCCAGTACCACAAATTCCAGAATCA
GCAGTTCAAGAAGCGATTCAAACCGTATTGCATCCAACTGCTAAGGCGATGATTGAAGAAAACCGTTCGTTTACAGGTAT
TTTATACGCAGGTCTTATTTTAACAAATGATGGTCCGAAGGTAATTGAATTTAATGCACGCTTCGGTGATCCTGAAACAG
AAGTTGTATTACCTCGATTAGAAAACGATTTAGTTGATGTATGTAACGCTGTATTAGATGAAAGTGAACTAACGTTACAA
TGGTCAGAAGAAGCGGTAATCGGTGTTGTACTTGCTTCTAAAGGATATCCAGAAGCATATAAAAAAGGTGATATTATTAG
CGGACTAGATGCACTGCAAGATGTGATTGTTTTCCATGCAGGTACAGCAATGAAACATGGGGACTTTGTAACGAACGGTG
GCCGTGTATTATTTGTTGCTTGTAAGGCAAATAGTTTACAAGAAGCAAAAGATAAAGTATATAAAGAAATCGGTAAAATT
GAGAGTGATGGTCTATTTTATCGAAGTGATATAGGATATCGTGCAATTGGGCATGAGATGACGAAAAGTTAA

Upstream 100 bases:

>100_bases
GTTTAAAAAGTCCGGTCGAGATAACCTTTTATTGATATGAAAAAGCAAACTAGGAGCATAGCTTCTAGTTTGTACTTATT
TAATTATTGGGGGATGAAAT

Downstream 100 bases:

>100_bases
TATGTAAAAATCCCGCTGAGGAATCAGCGGGATTTTTTATGCATCTTTATCTTTTTTTAATTTCCCTTTTTGCTTTGCCA
TTTCTCGAAGTAAATACTCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPVDIDENDFDALVLFAKENNVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYDIPTAAYETFTDYEEAVQYIQKVGAPIVIKADGLAAGKGVTVAMSLE
EALQAVKEMLQDVKFGAASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQIPES
AVQEAIQTVLHPTAKAMIEENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSEEAVIGVVLASKGYPEAYKKGDIISGLDALQDVIVFHAGTAMKHGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKI
ESDGLFYRSDIGYRAIGHEMTKS

Sequences:

>Translated_423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPVDIDENDFDALVLFAKENNVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYDIPTAAYETFTDYEEAVQYIQKVGAPIVIKADGLAAGKGVTVAMSLE
EALQAVKEMLQDVKFGAASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQIPES
AVQEAIQTVLHPTAKAMIEENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSEEAVIGVVLASKGYPEAYKKGDIISGLDALQDVIVFHAGTAMKHGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKI
ESDGLFYRSDIGYRAIGHEMTKS
>Mature_423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPVDIDENDFDALVLFAKENNVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYDIPTAAYETFTDYEEAVQYIQKVGAPIVIKADGLAAGKGVTVAMSLE
EALQAVKEMLQDVKFGAASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQIPES
AVQEAIQTVLHPTAKAMIEENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSEEAVIGVVLASKGYPEAYKKGDIISGLDALQDVIVFHAGTAMKHGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKI
ESDGLFYRSDIGYRAIGHEMTKS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=48.936170212766, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=48.936170212766, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=48.936170212766, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=48.936170212766, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=51.6355140186916, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=41.2322274881517, Blast_Score=310, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=43.4090909090909, Blast_Score=328, Evalue=8e-91,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=47.4418604651163, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=47.4418604651163, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46018; Mature: 46018

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPVDIDENDFDALVLFAKE
CEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEEEC
NNVELTFVGPEIPLMNGIVDRFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYDIPTAAY
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCHHHH
ETFTDYEEAVQYIQKVGAPIVIKADGLAAGKGVTVAMSLEEALQAVKEMLQDVKFGAASK
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCC
KVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQIPES
HHHHHHHCCCCCEEEEEEECCCEECEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCHH
AVQEAIQTVLHPTAKAMIEENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHH
ENDLVDVCNAVLDESELTLQWSEEAVIGVVLASKGYPEAYKKGDIISGLDALQDVIVFHA
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHC
GTAMKHGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKIESDGLFYRSDIGYRAIGHEM
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCHHH
TKS
HCC
>Mature Secondary Structure
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPVDIDENDFDALVLFAKE
CEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEEEC
NNVELTFVGPEIPLMNGIVDRFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYDIPTAAY
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCHHHH
ETFTDYEEAVQYIQKVGAPIVIKADGLAAGKGVTVAMSLEEALQAVKEMLQDVKFGAASK
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCC
KVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQIPES
HHHHHHHCCCCCEEEEEEECCCEECEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCHH
AVQEAIQTVLHPTAKAMIEENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHH
ENDLVDVCNAVLDESELTLQWSEEAVIGVVLASKGYPEAYKKGDIISGLDALQDVIVFHA
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHC
GTAMKHGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKIESDGLFYRSDIGYRAIGHEM
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCHHH
TKS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]