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The map label for this gene is acoL [H]

Identifier: 218231489

GI number: 218231489

Start: 2680781

End: 2682160

Strand: Reverse

Name: acoL [H]

Synonym: BCB4264_A2782

Alternate gene names: 218231489

Gene position: 2682160-2680781 (Counterclockwise)

Preceding gene: 218230914

Following gene: 218234884

Centisome position: 49.5

GC content: 38.48

Gene sequence:

>1380_bases
ATGAGTAAACTAGTTGTAATTGGAGGCGGACCAGCTGGATATGTAGCGGCAATTACCGCGGCTCAAAATGGCAAAGGTGT
CACTCTTATTGATGAAGCTGAACTTGGCGGAACGTGTTTAAATGTTGGTTGTATGCCTACGAAATCACTGTTAGAAAGTG
CTGAAATACATGACATTGTGAGAAAAGCGAATGATTATGGAGTTACGCTTAATACAGGAAGTATATCAATTGATTGGGAT
CAAATACAGGCGAGAAAATCACAAATCGTAATGCAACTCGTCCAAGGAATACAATATTTAATGAAGAAAAATAAAATTAA
AGTTGTAAAAGGAAAAGCGAAATTCGAAACGGATCATCGTGTGCGAGTTGTACAAGGAAATAAAGAAGAAGTAGTTGATG
GAGAGAGCTTTATTATCGCATCTGGTTCCGAACCTACAGAATTACCATTCGCTCCGTTCGATGGAAAATGGATTTTAAAT
AGTAGCCATGCCATGTCTCTTGAGAGTGTACCATCTTCATTGTTAATCGTTGGCGGTGGTGTAATAGGATGTGAGTTTGC
AAGTATTTATAGTAGACTTGGCACAAAAGTATCGATAGTTGAAATGGCACCGCAATTACTACCTGGTGAAGATGAAGATA
TCGCGAATATTTTAAGAGAGAAATTAGAAAAAGATGGTGTGAAAATTTTTACGGGAGCAGTTTTAAAAGGATTAAATAAC
TATAAGAAGCAAGCTTCATTTGAGTACGAGGGAATTACTCACGAAGTAACTCCAGAATTTGTTCTTGTTTCTGTAGGAAG
AAAACCACGAGTGCAACAATTAGGATTAGAAAAGGCAGGGGTTCAGTTTTCAAATAAAGGGATTTCTGTGAATGAACATA
TGCAAACGAACGTATCGCATATTTACGCAGCTGGTGATGTAATCGGTGGAATTCAGCTTGCTCACGTTGCCTTCCATGAA
GGAACGACAGCAGCATTACACGCAAGCGGAGAAGACGTGAAAGTAAATTATCACGCTGTGCCCCGTTGTATATACACAGC
TCCAGAAATAGCTAGTGTAGGGTTAAGTGAAAAAGGTGCAAGAGAACAATATGGTGATATTCTCATCGGAGAATTTCCTT
TTACAGCGAACGGAAAAGCTCTTATTTTAGGAGAACAAGTAGGTAAAGTAAAAGTCATTGTAGAACCTAAATACCAAGAA
ATTGTAGGGATTTCTATTATCGGTCCTTATGCAACTGAACTTATTGGTCAAGGAACCGTAATGATTCATACAGAAGTTAC
CGCTGATATAATGAGAGATTATATTGCAGCACATCCAACTTTATCTGAGGCGATTCATGAAGCGTTAATGCAAGCAGTAG
GACATGCTGTACATGCTTAA

Upstream 100 bases:

>100_bases
TCGTGTACTAGACGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACATTATTTAGAAGAACCTATAACAATTCTTT
TATAAAGAGAAGGTGAGAGA

Downstream 100 bases:

>100_bases
ATAGAAATATGATAAACCACTATTCTCTAAATGAAAGAGAGAATAGTGGTTTGAATTTTTATAGAAGAAATTAAATATCT
TATTACTATTTAATTTCAAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of acetoin cleaving system [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKLVVIGGGPAGYVAAITAAQNGKGVTLIDEAELGGTCLNVGCMPTKSLLESAEIHDIVRKANDYGVTLNTGSISIDWD
QIQARKSQIVMQLVQGIQYLMKKNKIKVVKGKAKFETDHRVRVVQGNKEEVVDGESFIIASGSEPTELPFAPFDGKWILN
SSHAMSLESVPSSLLIVGGGVIGCEFASIYSRLGTKVSIVEMAPQLLPGEDEDIANILREKLEKDGVKIFTGAVLKGLNN
YKKQASFEYEGITHEVTPEFVLVSVGRKPRVQQLGLEKAGVQFSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHE
GTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLSEKGAREQYGDILIGEFPFTANGKALILGEQVGKVKVIVEPKYQE
IVGISIIGPYATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALMQAVGHAVHA

Sequences:

>Translated_459_residues
MSKLVVIGGGPAGYVAAITAAQNGKGVTLIDEAELGGTCLNVGCMPTKSLLESAEIHDIVRKANDYGVTLNTGSISIDWD
QIQARKSQIVMQLVQGIQYLMKKNKIKVVKGKAKFETDHRVRVVQGNKEEVVDGESFIIASGSEPTELPFAPFDGKWILN
SSHAMSLESVPSSLLIVGGGVIGCEFASIYSRLGTKVSIVEMAPQLLPGEDEDIANILREKLEKDGVKIFTGAVLKGLNN
YKKQASFEYEGITHEVTPEFVLVSVGRKPRVQQLGLEKAGVQFSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHE
GTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLSEKGAREQYGDILIGEFPFTANGKALILGEQVGKVKVIVEPKYQE
IVGISIIGPYATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALMQAVGHAVHA
>Mature_458_residues
SKLVVIGGGPAGYVAAITAAQNGKGVTLIDEAELGGTCLNVGCMPTKSLLESAEIHDIVRKANDYGVTLNTGSISIDWDQ
IQARKSQIVMQLVQGIQYLMKKNKIKVVKGKAKFETDHRVRVVQGNKEEVVDGESFIIASGSEPTELPFAPFDGKWILNS
SHAMSLESVPSSLLIVGGGVIGCEFASIYSRLGTKVSIVEMAPQLLPGEDEDIANILREKLEKDGVKIFTGAVLKGLNNY
KKQASFEYEGITHEVTPEFVLVSVGRKPRVQQLGLEKAGVQFSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHEG
TTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLSEKGAREQYGDILIGEFPFTANGKALILGEQVGKVKVIVEPKYQEI
VGISIIGPYATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALMQAVGHAVHA

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=35, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI50301238, Length=447, Percent_Identity=29.3064876957494, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI148277065, Length=460, Percent_Identity=27.8260869565217, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI33519430, Length=463, Percent_Identity=28.0777537796976, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI33519428, Length=463, Percent_Identity=28.0777537796976, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI33519426, Length=463, Percent_Identity=28.0777537796976, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI148277071, Length=463, Percent_Identity=28.0777537796976, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=28.3842794759825, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=26.3888888888889, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI291045268, Length=423, Percent_Identity=24.3498817966903, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=34.075723830735, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=31.4977973568282, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=29.9303944315545, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=27.114967462039, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=37.1244635193133, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17557007, Length=466, Percent_Identity=26.8240343347639, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=439, Percent_Identity=26.1958997722096, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=439, Percent_Identity=26.1958997722096, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=473, Percent_Identity=25.369978858351, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=37.7118644067797, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6325166, Length=449, Percent_Identity=28.0623608017817, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=27.1008403361345, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=37.037037037037, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=27.9317697228145, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=27.9317697228145, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=27.9317697228145, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17737741, Length=466, Percent_Identity=27.4678111587983, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49190; Mature: 49059

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVVIGGGPAGYVAAITAAQNGKGVTLIDEAELGGTCLNVGCMPTKSLLESAEIHDIV
CCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKANDYGVTLNTGSISIDWDQIQARKSQIVMQLVQGIQYLMKKNKIKVVKGKAKFETDHR
HCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCE
VRVVQGNKEEVVDGESFIIASGSEPTELPFAPFDGKWILNSSHAMSLESVPSSLLIVGGG
EEEEECCCHHEECCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCEEEEEECC
VIGCEFASIYSRLGTKVSIVEMAPQLLPGEDEDIANILREKLEKDGVKIFTGAVLKGLNN
EEHHHHHHHHHHHCCEEEEEHHCHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
YKKQASFEYEGITHEVTPEFVLVSVGRKPRVQQLGLEKAGVQFSNKGISVNEHMQTNVSH
HHHHCCCEECCCCEECCCCEEEEECCCCCCHHHCCCHHHCCEECCCCCCCCHHHHCCCHH
IYAAGDVIGGIQLAHVAFHEGTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLSEKGA
EEECCHHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCHHHHCCCCCCCC
REQYGDILIGEFPFTANGKALILGEQVGKVKVIVEPKYQEIVGISIIGPYATELIGQGTV
HHHHCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECHHHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSEAIHEALMQAVGHAVHA
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLVVIGGGPAGYVAAITAAQNGKGVTLIDEAELGGTCLNVGCMPTKSLLESAEIHDIV
CEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKANDYGVTLNTGSISIDWDQIQARKSQIVMQLVQGIQYLMKKNKIKVVKGKAKFETDHR
HCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCE
VRVVQGNKEEVVDGESFIIASGSEPTELPFAPFDGKWILNSSHAMSLESVPSSLLIVGGG
EEEEECCCHHEECCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCEEEEEECC
VIGCEFASIYSRLGTKVSIVEMAPQLLPGEDEDIANILREKLEKDGVKIFTGAVLKGLNN
EEHHHHHHHHHHHCCEEEEEHHCHHHCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
YKKQASFEYEGITHEVTPEFVLVSVGRKPRVQQLGLEKAGVQFSNKGISVNEHMQTNVSH
HHHHCCCEECCCCEECCCCEEEEECCCCCCHHHCCCHHHCCEECCCCCCCCHHHHCCCHH
IYAAGDVIGGIQLAHVAFHEGTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLSEKGA
EEECCHHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCHHHHCCCCCCCC
REQYGDILIGEFPFTANGKALILGEQVGKVKVIVEPKYQEIVGISIIGPYATELIGQGTV
HHHHCCEEEECCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECHHHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSEAIHEALMQAVGHAVHA
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]