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The map label for this gene is acoC [H]

Identifier: 218230914

GI number: 218230914

Start: 2682176

End: 2683375

Strand: Reverse

Name: acoC [H]

Synonym: BCB4264_A2783

Alternate gene names: 218230914

Gene position: 2683375-2682176 (Counterclockwise)

Preceding gene: 218236134

Following gene: 218231489

Centisome position: 49.52

GC content: 36.08

Gene sequence:

>1200_bases
ATGGCTGTAGAAGTTGTAATGCCGAAGTTAGGTATGGCGATGAAAGAAGGTATTATTACGAGCTGGAATATTAAAGCAGG
CGATAATGTAGCAAAAGGAGAACTAATTGCTAGTATTAATTCAGAAAAAATTGAAACGGAAATTGAAGCGCCAGCTGATG
GTACGGTGCTTGATATAGCTGTAAGTGAAGATGAAGGGGTGCCGCCTGGTACTGTAATTTGCTACATCGGTAAACCGAAT
GAAAAAGTAGAAATGCAGGAAAGCACACATGTGATAGAAGAAAAAACATTTAATATAGAAGTACAAACTGTACAAAATCA
AGAACCAAATGGGAAAGAAGTAGCCAAGCAAAGAATTAAAATTTCACCTGTAGCGAAGAAAATTGCAAAGTCTGAAAATT
TGGATATCAAGGTATTAGTTGGTACAGGTCCTGGCGGAAGGATTACAAAGGTAGATGTATTAAAAGCACTTGAGGTAAGA
GGAATCGTTCCGGAAATATCTGAACAAGAAGAAAGTAACGTGATTCCTGTTACTGGTATGCGTAAAGCGATCGCAAATCG
TATGCACGCAAGCTTACAAAATAGCGCACAATTAACGTTAACGATGAAAGTAGATGTAACAGATTTAGTTGCTTTACATA
AAGAAATAGCGAAAGTTGTACAAAAAAGATATGATAATAAATTAACCATTACGGATTTCGTCTCGCGTGCTGCTGTATTA
GCACTTGGAGAGCATAAAGAAATGAACAGCGCTTATATAGATGATGCAATTCATCAATTTGAACATGTGCATTTAGGCAT
GGCAGTCGCATTAGAAAAAGGGTTAATCGTTCCAGCTATTCGCTTTGCAAATAATTTATCTTTAGTAGAATTGTCTAAAG
AGATTAAGAATGTGGCACAAAAGGCGCGAGCAGGTAGCTTAAGTAGTGATGATATGCACGGAACGACATTTACAATTAGT
AATTTAGGTAGCTTTGGTATTGAATATTTCACACCTGTATTAAATACACCTGAGACTGGCATTTTAGGTATTGGCGCAAT
TGAACATGTTCCTGTATATAAAGGGAAAAAATTAAAAAAAGGTAGTATGTTACCTTTAAGTTTAACATTCGATCATCGTG
TACTAGACGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACATTATTTAGAAGAACCTATAACAATTCTTTTATAA

Upstream 100 bases:

>100_bases
TTTGCCAACGCCAGAAAAAGTAATTGAAACTGTATCAGAAATGATTGGAGACCAATCTTTATTACATGTGTAATAAAACG
ATGAGAAGGGAGAGAGAAAC

Downstream 100 bases:

>100_bases
AGAGAAGGTGAGAGAATGAGTAAACTAGTTGTAATTGGAGGCGGACCAGCTGGATATGTAGCGGCAATTACCGCGGCTCA
AAATGGCAAAGGTGTCACTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPN
EKVEMQESTHVIEEKTFNIEVQTVQNQEPNGKEVAKQRIKISPVAKKIAKSENLDIKVLVGTGPGGRITKVDVLKALEVR
GIVPEISEQEESNVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYDNKLTITDFVSRAAVL
ALGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLIVPAIRFANNLSLVELSKEIKNVAQKARAGSLSSDDMHGTTFTIS
NLGSFGIEYFTPVLNTPETGILGIGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKHYLEEPITILL

Sequences:

>Translated_399_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPN
EKVEMQESTHVIEEKTFNIEVQTVQNQEPNGKEVAKQRIKISPVAKKIAKSENLDIKVLVGTGPGGRITKVDVLKALEVR
GIVPEISEQEESNVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYDNKLTITDFVSRAAVL
ALGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLIVPAIRFANNLSLVELSKEIKNVAQKARAGSLSSDDMHGTTFTIS
NLGSFGIEYFTPVLNTPETGILGIGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKHYLEEPITILL
>Mature_398_residues
AVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPNE
KVEMQESTHVIEEKTFNIEVQTVQNQEPNGKEVAKQRIKISPVAKKIAKSENLDIKVLVGTGPGGRITKVDVLKALEVRG
IVPEISEQEESNVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYDNKLTITDFVSRAAVLA
LGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLIVPAIRFANNLSLVELSKEIKNVAQKARAGSLSSDDMHGTTFTISN
LGSFGIEYFTPVLNTPETGILGIGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKHYLEEPITILL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=30.6264501160093, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=27.1028037383178, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.6075388026608, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=26.6075388026608, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI19923748, Length=223, Percent_Identity=34.5291479820628, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI260898739, Length=180, Percent_Identity=32.2222222222222, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786946, Length=413, Percent_Identity=30.5084745762712, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786305, Length=419, Percent_Identity=27.6849642004773, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=30.2325581395349, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=399, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=25.2927400468384, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=26.8608414239482, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=29.3827160493827, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=27.212389380531, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=31.2714776632302, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI20129315, Length=291, Percent_Identity=31.2714776632302, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=25.5172413793103, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645909, Length=253, Percent_Identity=32.8063241106719, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43361; Mature: 43230

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIA
CCEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCEEEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEMQESTHVIEEKTFNIEVQTVQNQEPNGKEVAKQRIK
ECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHCEEEEEEEEEECCCCCCHHHHHHHHCC
ISPVAKKIAKSENLDIKVLVGTGPGGRITKVDVLKALEVRGIVPEISEQEESNVIPVTGM
CCHHHHHHHCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCEECCCHH
RKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYDNKLTITDFVSRAAVL
HHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
ALGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLIVPAIRFANNLSLVELSKEIKNVAQ
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCHHHHHHHHHHHHHH
KARAGSLSSDDMHGTTFTISNLGSFGIEYFTPVLNTPETGILGIGAIEHVPVYKGKKLKK
HHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCC
GSMLPLSLTFDHRVLDGAPAAAFLRTIKHYLEEPITILL
CCCCEEEEEECCEECCCCCHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
AVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTVLDIA
CEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCEEEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEMQESTHVIEEKTFNIEVQTVQNQEPNGKEVAKQRIK
ECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHCEEEEEEEEEECCCCCCHHHHHHHHCC
ISPVAKKIAKSENLDIKVLVGTGPGGRITKVDVLKALEVRGIVPEISEQEESNVIPVTGM
CCHHHHHHHCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCEECCCHH
RKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYDNKLTITDFVSRAAVL
HHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
ALGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLIVPAIRFANNLSLVELSKEIKNVAQ
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCHHHHHHHHHHHHHH
KARAGSLSSDDMHGTTFTISNLGSFGIEYFTPVLNTPETGILGIGAIEHVPVYKGKKLKK
HHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCC
GSMLPLSLTFDHRVLDGAPAAAFLRTIKHYLEEPITILL
CCCCEEEEEECCEECCCCCHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]