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The map label for this gene is gid [H]

Identifier: 218231463

GI number: 218231463

Start: 3851664

End: 3852968

Strand: Reverse

Name: gid [H]

Synonym: BCB4264_A3930

Alternate gene names: 218231463

Gene position: 3852968-3851664 (Counterclockwise)

Preceding gene: 218232018

Following gene: 218235700

Centisome position: 71.1

GC content: 39.46

Gene sequence:

>1305_bases
ATGACAACACAAGTAGTAAACGTCATTGGCGCAGGTCTTGCAGGAAGTGAAGCGGCTTACCAAATTGCAAAACGTGGTGT
CCAAGTTAGATTATATGAAATGAGACCGGTAAGGCAAACGCCAGCTCATCACACAGATAAATTCGCTGAATTAGTATGTA
GTAACTCACTTCGTGCAAACACTTTAACAAATGCAGTTGGAGTTATTAAAGAGGAAATGCGCTTAATGGATTCTGTAATT
ATTCGTGCGGCGGATGAGTGCTCTGTACCAGCTGGAGGTGCTTTAGCTGTAGATCGTCATGAGTTCGCAGCTAAAGTAAC
AGAATACGTAAAGAACCATCCAAATGTAACTGTAATGAATGAAGAAATTACAGATATTCCAGAGGGACCGACAATTATTG
CTACAGGTCCATTAACATCTCCTGATCTTTCTGCAAAATTGAAAGAATTAACAGGAGAAGATTATTTCTACTTCTATGAT
GCTGCAGCCCCAATCGTAGAAAAAGACAGTATTGACATGAATAAAGTATATTTAAAATCGCGTTATGACAAAGGTGAAGC
TGCATATTTAAATTGCCCAATGACAGAAGAAGAGTTTGATCGTTTTTATGAGGCTCTAATTGCTGCTGAGACAGTGCCTT
TAAAAGAGTTTGAAAAAGAAATTTTCTTTGAAGGTTGTATGCCAGTAGAAGTTATGGCAAGTAGAGGGAGACAGACGTTA
GTATTTGGACCTATGAAACCTGTTGGATTGGAAGATCCAAAAACAGGGAAAACGCCATATGCTGTTGTTCAGTTACGTCA
AGATGATGCAGCGGGAACTTTATACAATATCGTAGGTTTCCAAACTCATTTAAAGTGGGGACCGCAAAAAGAAGTGCTAC
AGTTAATTCCGGGATTGGAAAATGCAGAGATTGTACGTTATGGTGTAATGCATCGTAATACGTTTATTAATTCACCTAAT
TTGCTTCGTCCAACATATCAATATAAACAACGTGATGATTTATTCTTCGCTGGTCAAATGACTGGAGTAGAGGGATATGT
TGAATCAGCAGCATCAGGATTATTAGCAGGAATTAATGCTGCGCGTCTTGTGCAAGGTGAAGAGCCAGTTGTATTACCGC
CAGTAACTGCGATGGGTAGCATGGCAAATTACATTACGGCAACGAACGCTAAAAACTTCCAGCCGATGAATGCGAACTTC
GGTCTATTTGCACCGTTAGAGAAGAAGATTAAAAAGAAAGCAGAACGAAATGAAGCGTACGCAGCACGCGCTTTAGAAAC
GATTCGAAATTTTGTAAATATTTAG

Upstream 100 bases:

>100_bases
CGTGCAAGTACAATGTATTTCGTGCGATTATGAAGAAGAACAACAAATGTGAGCGTAACTGCTCACATTTTTTTCGGGAA
GAAAAGGAAAGGTGATATAA

Downstream 100 bases:

>100_bases
TTAAATTTCTTGCAAAGGCTACTAAGTTGTGATACGATTTAGTAGCCTTTATTGAGGTGAGTTGTGTGAATGTGAAGAAA
TTGTTACAATTATTCGTTGG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITDIPEGPTIIATGPLTSPDLSAKLKELTGEDYFYFYD
AAAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPN
LLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVQGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANF
GLFAPLEKKIKKKAERNEAYAARALETIRNFVNI

Sequences:

>Translated_434_residues
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITDIPEGPTIIATGPLTSPDLSAKLKELTGEDYFYFYD
AAAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPN
LLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVQGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANF
GLFAPLEKKIKKKAERNEAYAARALETIRNFVNI
>Mature_433_residues
TTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVII
RAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITDIPEGPTIIATGPLTSPDLSAKLKELTGEDYFYFYDA
AAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTLV
FGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPNL
LRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVQGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFG
LFAPLEKKIKKKAERNEAYAARALETIRNFVNI

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=45.5555555555556, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17534255, Length=131, Percent_Identity=35.1145038167939, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321202, Length=172, Percent_Identity=30.2325581395349, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 47995; Mature: 47863

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRAN
CCCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRLMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITDIPEGPTIIATGPLTSPDLSAKLKELTGEDYFYFYDAAAPIVEKDSIDMNKVYLKS
CHHCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPNLLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLVQGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFGLFAPLEKKIKKKAERNEAY
HHEECCCCCEEECCHHHHHHHHHHEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
AARALETIRNFVNI
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRAN
CCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRLMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITDIPEGPTIIATGPLTSPDLSAKLKELTGEDYFYFYDAAAPIVEKDSIDMNKVYLKS
CHHCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPNLLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLVQGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFGLFAPLEKKIKKKAERNEAY
HHEECCCCCEEECCHHHHHHHHHHEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
AARALETIRNFVNI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA