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The map label for this gene is ldh [H]

Identifier: 218231441

GI number: 218231441

Start: 1838468

End: 1839412

Strand: Reverse

Name: ldh [H]

Synonym: BCB4264_A1927

Alternate gene names: 218231441

Gene position: 1839412-1838468 (Counterclockwise)

Preceding gene: 218234079

Following gene: 218230893

Centisome position: 33.94

GC content: 38.41

Gene sequence:

>945_bases
ATGAAAAAAGGTATTAACCGTGTTGTATTAGTAGGAACAGGAGCAGTTGGATGTAGTTATGCTTACTGCATGATTAACCA
GGCTGTAGCTGAAGAATTTGTTTTAGTCGATGTAAATGAGGCAAAAGCTGAAGGGGAAGCAATGGACTTAAGTCATGCTG
TTCCATTCGCACCAGCTCCAACTAGAGTATGGAAAGGCAGCTACGAAGATTGTAAAGATGCTGACCTTGTAGTTATTACA
GCTGGATTACCACAAAAACCAGGCGAAACACGTTTAGATTTAGTTGAGAAAAACGCTAAAATCTTCAAACAAATCGTTCG
CAGTATTATGGATAGCGGATTTGATGGCATCTTCTTAATCGCAACAAACCCTGTAGATATTTTAACTTACGTAACTTGGA
AAGAATCTGGTTTACCGAAAGAACGTGTAATCGGTTCTGGTACAACGCTTGATTCTGCTCGTTTCCGCTATATGTTAGGT
GAGTACTTCGATATTGGTCCACACAACATTCACGCTTATATTATCGGAGAACACGGCGATACTGAACTTCCAGTTTGGAG
CCATGTTTCCGTTGGTATCCAAAAACTACAAACACTGCTTGAAAAAGACAACACGTATAATCAAGAAGATTTAGACAAAA
TTTTCATAAACGTCCGTGATGCAGCTTACCATATTATTGAGCGTAAAGGCGCAACTTATTATGGTATCGGTATGTCACTT
CTACGTGTTACAAAAGCAATTTTAAACGACGAAAATAGTGTATTAACTGTATCAGCTTACTTAGAAGGTCAATACGGTCA
AAAAGATGTTTATATCGGGGTACCTGCTGTTTTAAATCGCGGCGGTGTTCGTGAAATTTTAGAGGTTGAATTAAGTGAAG
ATGAAGAATTAAAATTCGACCACTCTGTTCAAGTATTGAAAGAAACAATGGCTCCGGTTCTTTAA

Upstream 100 bases:

>100_bases
GAATATCTTTTATAAAATGTAATAAACTAGATATGACTCTATAGAAGTAAGACGTATTATCTTACATAATAAAATCAATG
AAATTGCGAGGAATTTTGAA

Downstream 100 bases:

>100_bases
TTAAGATAAAAAGCAATCCAATTTATTTTGGATTGCTTTTTATTTTCATTATCTCCTATGACTTTTTAGTGATTTTCTAT
CATTTCTTATTTTTTAGTCG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 1 [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAPTRVWKGSYEDCKDADLVVIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
EYFDIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFDHSVQVLKETMAPVL

Sequences:

>Translated_314_residues
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAPTRVWKGSYEDCKDADLVVIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
EYFDIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFDHSVQVLKETMAPVL
>Mature_314_residues
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAPTRVWKGSYEDCKDADLVVIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
EYFDIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFDHSVQVLKETMAPVL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=41.6393442622951, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=41.6393442622951, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI260099723, Length=303, Percent_Identity=40.9240924092409, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI5031857, Length=303, Percent_Identity=40.9240924092409, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=40.2640264026403, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=40.2640264026403, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI9257228, Length=302, Percent_Identity=41.3907284768212, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI4504973, Length=302, Percent_Identity=41.3907284768212, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=38.3606557377049, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=47.4226804123711, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI260099725, Length=194, Percent_Identity=47.4226804123711, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI207028494, Length=204, Percent_Identity=39.7058823529412, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI103472011, Length=293, Percent_Identity=25.938566552901, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=28.6486486486486, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI21735621, Length=318, Percent_Identity=24.5283018867925, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1789632, Length=323, Percent_Identity=27.2445820433437, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535107, Length=294, Percent_Identity=40.8163265306122, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17554310, Length=318, Percent_Identity=27.9874213836478, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322765, Length=193, Percent_Identity=30.0518134715026, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=38.961038961039, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=28.9902280130293, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24647881, Length=305, Percent_Identity=27.2131147540984, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663599, Length=326, Percent_Identity=22.6993865030675, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34789; Mature: 34789

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAP
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
TRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLI
CHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFDIGPHNIHAYIIGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCC
TELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEHHHHHHHHHHHCCCEEEHHHHHH
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFD
HHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCCHHHHHEEECCCCCCCCCH
HSVQVLKETMAPVL
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAP
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
TRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLI
CHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFDIGPHNIHAYIIGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCC
TELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSL
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEHHHHHHHHHHHCCCEEEHHHHHH
LRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFD
HHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCCHHHHHEEECCCCCCCCCH
HSVQVLKETMAPVL
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]