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The map label for this gene is sbp [H]

Identifier: 218231228

GI number: 218231228

Start: 1101896

End: 1102951

Strand: Direct

Name: sbp [H]

Synonym: BCB4264_A1125

Alternate gene names: 218231228

Gene position: 1101896-1102951 (Clockwise)

Preceding gene: 218236080

Following gene: 218232070

Centisome position: 20.33

GC content: 37.41

Gene sequence:

>1056_bases
ATGAAAAAGTGGGGTAAGGCAGCGTTTATGAAAAGTACAGGTGTTTTATTATCAGCAGTTGTACTTTTATCAGGGTGTGG
TTCGGCAACATCCACTAGTAATTCTGAAGGAAGTGGGGATAAAAAAACAGTTGAGCTTTTAAATGTTTCTTATGATCCAA
CTAGGGAGTTATATCAAGATTTTAATAAAGATTTTGCGAAGTATTGGAAAGAGAAGCACGGGCAAACAGTAAATGTAAAA
CAATCACACGGGGGATCTGGAAGTCAGGCGCGTTCTGTTATTGATGGCTTAGAAGCAGACGTTGTTACACTAGCACTTGC
TTATGATATTGATGCCATTAGTAAGAAAAAACAACTAGCAGAAGATTGGCAGAAGCGACTTGCGAATAATTCCACTCCAT
ACACGTCAACAATTGTATTTCTTGTTCGAAAAGGAAACCCGAAAGGAATTAAAGACTGGGATGATTTAACGAAAAAAGGT
GTGTCTGTCATTACACCAAACCCAAAAACATCAGGCGGAGCACGTTGGAACTATTTAGCGGCGTGGGGATACGCATTGAA
AAAATATAATAATAGTGAAGATAAGGCGAAAGAGTTTGTTAGCCAAATTTATAAAAACGTAGAAGTATTAGATTCAGGAG
CGCGCGGAGCAACGACAACATTTGTTGAAAAAGGAATTGGTGACGTATTAATCGCATGGGAAAATGAAGCGTTATTATCC
CAAAAAGAGCTTGGAAAAGATAAATTTGAAATTGTAACCCCTTCTATTAGTGTACTTGCAGAACCACCAGTAGCGGTTGT
AGATAAAGTCGTTGATAAAAAAGGAACGAAAAAAGTAGCAGAAGGGTATCTCGAATATTTGTACTCAGAAAAAGGACAGG
AAATTGCGGCAAAAAACTTTTATCGTCCGCGTAATGAAAAAGTAGCAGAAAAATATGCATCCCAATTTCCGAAAGTTCAA
TTATTTACTGTAGATGAACTATTTGGTGGTTGGAAAAAAGCACAGGAGAAACACCTTAATGACGGCGGCGTATTCGATAA
AATTTATCAAAAATAA

Upstream 100 bases:

>100_bases
TACATTAGAAGCTAATCAGATAAACTGAAGGCGCACAATGTTCACATTTGTGAATATATTGTGTGCCTTTTTCTATTGTA
TAAACTAAGGGGGCTTAACG

Downstream 100 bases:

>100_bases
TGCGTACTGCTAAAGGGGGATGAGGTGAAAGAGGATGAGGAAGAAACGTGTTTTACCAGGGTTTGGATTATCGCTTGGAT
TTACAATGTTGTACATGAGT

Product: sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKWGKAAFMKSTGVLLSAVVLLSGCGSATSTSNSEGSGDKKTVELLNVSYDPTRELYQDFNKDFAKYWKEKHGQTVNVK
QSHGGSGSQARSVIDGLEADVVTLALAYDIDAISKKKQLAEDWQKRLANNSTPYTSTIVFLVRKGNPKGIKDWDDLTKKG
VSVITPNPKTSGGARWNYLAAWGYALKKYNNSEDKAKEFVSQIYKNVEVLDSGARGATTTFVEKGIGDVLIAWENEALLS
QKELGKDKFEIVTPSISVLAEPPVAVVDKVVDKKGTKKVAEGYLEYLYSEKGQEIAAKNFYRPRNEKVAEKYASQFPKVQ
LFTVDELFGGWKKAQEKHLNDGGVFDKIYQK

Sequences:

>Translated_351_residues
MKKWGKAAFMKSTGVLLSAVVLLSGCGSATSTSNSEGSGDKKTVELLNVSYDPTRELYQDFNKDFAKYWKEKHGQTVNVK
QSHGGSGSQARSVIDGLEADVVTLALAYDIDAISKKKQLAEDWQKRLANNSTPYTSTIVFLVRKGNPKGIKDWDDLTKKG
VSVITPNPKTSGGARWNYLAAWGYALKKYNNSEDKAKEFVSQIYKNVEVLDSGARGATTTFVEKGIGDVLIAWENEALLS
QKELGKDKFEIVTPSISVLAEPPVAVVDKVVDKKGTKKVAEGYLEYLYSEKGQEIAAKNFYRPRNEKVAEKYASQFPKVQ
LFTVDELFGGWKKAQEKHLNDGGVFDKIYQK
>Mature_351_residues
MKKWGKAAFMKSTGVLLSAVVLLSGCGSATSTSNSEGSGDKKTVELLNVSYDPTRELYQDFNKDFAKYWKEKHGQTVNVK
QSHGGSGSQARSVIDGLEADVVTLALAYDIDAISKKKQLAEDWQKRLANNSTPYTSTIVFLVRKGNPKGIKDWDDLTKKG
VSVITPNPKTSGGARWNYLAAWGYALKKYNNSEDKAKEFVSQIYKNVEVLDSGARGATTTFVEKGIGDVLIAWENEALLS
QKELGKDKFEIVTPSISVLAEPPVAVVDKVVDKKGTKKVAEGYLEYLYSEKGQEIAAKNFYRPRNEKVAEKYASQFPKVQ
LFTVDELFGGWKKAQEKHLNDGGVFDKIYQK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=310, Percent_Identity=70.3225806451613, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1788765, Length=312, Percent_Identity=47.4358974358974, Blast_Score=292, Evalue=2e-80,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38865; Mature: 38865

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWGKAAFMKSTGVLLSAVVLLSGCGSATSTSNSEGSGDKKTVELLNVSYDPTRELYQD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEECCCCCHHHHHHH
FNKDFAKYWKEKHGQTVNVKQSHGGSGSQARSVIDGLEADVVTLALAYDIDAISKKKQLA
HHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDWQKRLANNSTPYTSTIVFLVRKGNPKGIKDWDDLTKKGVSVITPNPKTSGGARWNYLA
HHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCCHHHH
AWGYALKKYNNSEDKAKEFVSQIYKNVEVLDSGARGATTTFVEKGIGDVLIAWENEALLS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCHHHHH
QKELGKDKFEIVTPSISVLAEPPVAVVDKVVDKKGTKKVAEGYLEYLYSEKGQEIAAKNF
HHHCCCCCEEEECCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHC
YRPRNEKVAEKYASQFPKVQLFTVDELFGGWKKAQEKHLNDGGVFDKIYQK
CCCCCHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MKKWGKAAFMKSTGVLLSAVVLLSGCGSATSTSNSEGSGDKKTVELLNVSYDPTRELYQD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEECCCCCHHHHHHH
FNKDFAKYWKEKHGQTVNVKQSHGGSGSQARSVIDGLEADVVTLALAYDIDAISKKKQLA
HHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDWQKRLANNSTPYTSTIVFLVRKGNPKGIKDWDDLTKKGVSVITPNPKTSGGARWNYLA
HHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCCHHHH
AWGYALKKYNNSEDKAKEFVSQIYKNVEVLDSGARGATTTFVEKGIGDVLIAWENEALLS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCHHHHH
QKELGKDKFEIVTPSISVLAEPPVAVVDKVVDKKGTKKVAEGYLEYLYSEKGQEIAAKNF
HHHCCCCCEEEECCCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHC
YRPRNEKVAEKYASQFPKVQLFTVDELFGGWKKAQEKHLNDGGVFDKIYQK
CCCCCHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]