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The map label for this gene is cysT [H]

Identifier: 218232070

GI number: 218232070

Start: 1102986

End: 1103813

Strand: Direct

Name: cysT [H]

Synonym: BCB4264_A1126

Alternate gene names: 218232070

Gene position: 1102986-1103813 (Clockwise)

Preceding gene: 218231228

Following gene: 218235428

Centisome position: 20.35

GC content: 38.89

Gene sequence:

>828_bases
ATGAGGAAGAAACGTGTTTTACCAGGGTTTGGATTATCGCTTGGATTTACAATGTTGTACATGAGTTTATTCGTACTTAT
ACCACTTTCTATCGTATTTATTCAAACTTCACAGTTAGGTTGGAAGAAGTTCGCTGGGGTTATAACGAACGAGCGTGTGT
TGCATTCCTATCAAGTTAGTTTTACAACTTCATTGGTAGCGGCTATTGTAAATGCCATTTTCGGTTTATTAATAGCTTGG
GTGCTCGTACGCTACAAATTTCCAGGGAAACGACTATTAGATGGACTCATTGATTTACCGTTTGCACTTCCGACAGCTGT
AGCTGGTATTACACTTACAACGCTTTATGCGGAAAATGGATGGATTGGGAAAATATTTAGTCTGTTTCATATAAAAGTAG
CTTTTACACCACTCGGAATTATCGTTGCGCTTACTTTTATCGGTTTACCATTTGTCGTTCGAATGGTGCAACCAGTACTG
CAAAATATTGATAAAGAAGTAGAAGAGGCAGCTTCGAGTTTAGGTGCATCCCGCTTGCAAATATTTGTGAAAATCATTTT
ACCAGAAATCTTTCCCGCTCTTCTTGCCGGTTTTTCACTCGCATTTGCAAGAGCATTAGGAGAATACGGATCTGTCGTTT
TCATCGCAGGTAATATGCCGATGAAAACAGAAATTGCACCACTGATGATTATGACGAAACTAGAACAATATGATTATGCG
GGGGCAACAGCTGTAGCGACCGTGATGCTTATTATTTCTCTCCTTTTCCTTCTTCTTATTAATATGATTCAAAGCTGGAG
TAGAAGGCATGAACTAAAAAGTGAATAG

Upstream 100 bases:

>100_bases
TGGAAAAAAGCACAGGAGAAACACCTTAATGACGGCGGCGTATTCGATAAAATTTATCAAAAATAATGCGTACTGCTAAA
GGGGGATGAGGTGAAAGAGG

Downstream 100 bases:

>100_bases
GGGGAGAGAAAATGGAACCAACAGTATTAGAAAAGAAAATAGTAAAAAGCGTATCAGCTAAAAAAGAATCTAAGCTTGTA
CCAGGCGTATTAATTACGAT

Product: sulfate ABC transporter, permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRKKRVLPGFGLSLGFTMLYMSLFVLIPLSIVFIQTSQLGWKKFAGVITNERVLHSYQVSFTTSLVAAIVNAIFGLLIAW
VLVRYKFPGKRLLDGLIDLPFALPTAVAGITLTTLYAENGWIGKIFSLFHIKVAFTPLGIIVALTFIGLPFVVRMVQPVL
QNIDKEVEEAASSLGASRLQIFVKIILPEIFPALLAGFSLAFARALGEYGSVVFIAGNMPMKTEIAPLMIMTKLEQYDYA
GATAVATVMLIISLLFLLLINMIQSWSRRHELKSE

Sequences:

>Translated_275_residues
MRKKRVLPGFGLSLGFTMLYMSLFVLIPLSIVFIQTSQLGWKKFAGVITNERVLHSYQVSFTTSLVAAIVNAIFGLLIAW
VLVRYKFPGKRLLDGLIDLPFALPTAVAGITLTTLYAENGWIGKIFSLFHIKVAFTPLGIIVALTFIGLPFVVRMVQPVL
QNIDKEVEEAASSLGASRLQIFVKIILPEIFPALLAGFSLAFARALGEYGSVVFIAGNMPMKTEIAPLMIMTKLEQYDYA
GATAVATVMLIISLLFLLLINMIQSWSRRHELKSE
>Mature_275_residues
MRKKRVLPGFGLSLGFTMLYMSLFVLIPLSIVFIQTSQLGWKKFAGVITNERVLHSYQVSFTTSLVAAIVNAIFGLLIAW
VLVRYKFPGKRLLDGLIDLPFALPTAVAGITLTTLYAENGWIGKIFSLFHIKVAFTPLGIIVALTFIGLPFVVRMVQPVL
QNIDKEVEEAASSLGASRLQIFVKIILPEIFPALLAGFSLAFARALGEYGSVVFIAGNMPMKTEIAPLMIMTKLEQYDYA
GATAVATVMLIISLLFLLLINMIQSWSRRHELKSE

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=263, Percent_Identity=53.2319391634981, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87082099, Length=260, Percent_Identity=32.6923076923077, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1786980, Length=212, Percent_Identity=34.4339622641509, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787368, Length=222, Percent_Identity=31.5315315315315, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787369, Length=166, Percent_Identity=31.3253012048193, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786254, Length=221, Percent_Identity=24.4343891402715, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787081, Length=253, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30389; Mature: 30389

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]