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The map label for this gene is pykA1 [H]

Identifier: 218230905

GI number: 218230905

Start: 3214302

End: 3215360

Strand: Reverse

Name: pykA1 [H]

Synonym: BCB4264_A3335

Alternate gene names: 218230905

Gene position: 3215360-3214302 (Counterclockwise)

Preceding gene: 218231171

Following gene: 218231796

Centisome position: 59.33

GC content: 35.13

Gene sequence:

>1059_bases
ATGACAATCGATCGAATTTGTACAATTGGACCCGCAAGTAATAATAAAGAAACGTTAGCACAGTTAATAAAGAATGGTAT
GAAAATTGTTCGACTGAATTTATCGCATGGCACGCATGAAAGTCATAAAGACATCATTCGTTTAGTGAAATCATTAGATG
ACTCTATTAAATTTTTGGGTGATGTACAAGGACCTAAAATAAGATTAGGTGAAGTGAAGGGAGAGCAAATTACACTTCAG
GCAGGAGATTCTTTTATTTTACATACACAATCAGTTACAGGGAGTAATACAGAAGCGAGTGTTGATTATGAAGGAATTGC
GAATGATGTAAAAGTAGGCAGTAGAATTTTAATTAATGATGGAGAAGTTGAATTAATTGTTGAAAAGATAAGTACGGACA
AAATAGAAACGAAGGTAAAAACAGGTGGCAATATCTCATCTCATAAAGGAGTTAATTTACCAGATGCGGCTGTTCGTTTA
CCAGCTATTACAGAGAAAGATAAAAAAGATATTCAGTTTCTTTTAGAGGAGAATGTTGATTTTATCGCGTGTTCTTTCGT
AAGAAAACCTAGTCATATAAAAGAAATACGAGATTTTATACAACAGTATAAAGAAACGTCACCTAATATAATTGCAAAAA
TAGAAACGATGGAAGCAATCGAGAATTTTCAAGATATATGTAAAGAAGCAGATGGAATTATGATTGCAAGGGGCGATTTA
GGAGTAGAGTTACCGTATCAATGCATTCCGCTTTTACAGAAAATAATGATTCAGGAATGTAATCGAACGAATACATATGT
CATTACAGCAACACAAATGCTTCAATCTATGGTAGATCATTCTATTCCAACAAGGGCAGAGGTGACTGATGTGTTTCAAG
CTGTACTGGACGGAACGAATGCAGTTATGCTTTCAGCTGAAAGTGCATCAGGAGAGCATCCAATCGAAAGTGTAAGAACA
TTACGTCTCGTTTCAGAATTTGCGGAACATGTTAAAAAAGATGGACCTTTTGCGATGAAAGATGTACTGCAATTACTGCG
TGAGTCTCTGGATGAGTAA

Upstream 100 bases:

>100_bases
ACTTTATGAAATTCGTGTAAATTTAAATCTATTCATGTCAGAATCTCTGTTATTTTTAAGTTCATATGGGAAACTTATTA
AAAAAAGGATGGTGACAAAA

Downstream 100 bases:

>100_bases
TAAATAAAAAGAGGGCGGATATACATCCGCCCGCGTGTTTTTCATTCATACTATTCTTGCTCTTGCTCATCCAAAATCTT
TTCAAACGCAGCAGATACTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTIDRICTIGPASNNKETLAQLIKNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKFLGDVQGPKIRLGEVKGEQITLQ
AGDSFILHTQSVTGSNTEASVDYEGIANDVKVGSRILINDGEVELIVEKISTDKIETKVKTGGNISSHKGVNLPDAAVRL
PAITEKDKKDIQFLLEENVDFIACSFVRKPSHIKEIRDFIQQYKETSPNIIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPYQCIPLLQKIMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPIESVRT
LRLVSEFAEHVKKDGPFAMKDVLQLLRESLDE

Sequences:

>Translated_352_residues
MTIDRICTIGPASNNKETLAQLIKNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKFLGDVQGPKIRLGEVKGEQITLQ
AGDSFILHTQSVTGSNTEASVDYEGIANDVKVGSRILINDGEVELIVEKISTDKIETKVKTGGNISSHKGVNLPDAAVRL
PAITEKDKKDIQFLLEENVDFIACSFVRKPSHIKEIRDFIQQYKETSPNIIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPYQCIPLLQKIMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPIESVRT
LRLVSEFAEHVKKDGPFAMKDVLQLLRESLDE
>Mature_351_residues
TIDRICTIGPASNNKETLAQLIKNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKFLGDVQGPKIRLGEVKGEQITLQA
GDSFILHTQSVTGSNTEASVDYEGIANDVKVGSRILINDGEVELIVEKISTDKIETKVKTGGNISSHKGVNLPDAAVRLP
AITEKDKKDIQFLLEENVDFIACSFVRKPSHIKEIRDFIQQYKETSPNIIAKIETMEAIENFQDICKEADGIMIARGDLG
VELPYQCIPLLQKIMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPIESVRTL
RLVSEFAEHVKKDGPFAMKDVLQLLRESLDE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=340, Percent_Identity=42.9411764705882, Blast_Score=276, Evalue=1e-74,
Organism=Homo sapiens, GI33286420, Length=340, Percent_Identity=42.9411764705882, Blast_Score=276, Evalue=1e-74,
Organism=Homo sapiens, GI33286418, Length=340, Percent_Identity=42.9411764705882, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI10835121, Length=347, Percent_Identity=41.2103746397695, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI32967597, Length=347, Percent_Identity=41.2103746397695, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=40.9893992932862, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=40.9893992932862, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI310128736, Length=231, Percent_Identity=38.961038961039, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128734, Length=231, Percent_Identity=38.961038961039, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=36.7441860465116, Blast_Score=138, Evalue=9e-33,
Organism=Escherichia coli, GI1787965, Length=332, Percent_Identity=42.4698795180723, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1788160, Length=335, Percent_Identity=39.1044776119403, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17506829, Length=336, Percent_Identity=45.8333333333333, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=336, Percent_Identity=45.8333333333333, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71984406, Length=336, Percent_Identity=45.8333333333333, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=336, Percent_Identity=45.8333333333333, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17544584, Length=350, Percent_Identity=39.7142857142857, Blast_Score=247, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=336, Percent_Identity=38.6904761904762, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324923, Length=336, Percent_Identity=36.0119047619048, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=42.3529411764706, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=42.3529411764706, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24648966, Length=322, Percent_Identity=39.1304347826087, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24581235, Length=339, Percent_Identity=35.3982300884956, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24646914, Length=192, Percent_Identity=39.0625, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 38980; Mature: 38849

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDRICTIGPASNNKETLAQLIKNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKFLG
CCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
DVQGPKIRLGEVKGEQITLQAGDSFILHTQSVTGSNTEASVDYEGIANDVKVGSRILIND
CCCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCCHHHCCCCEEEEEC
GEVELIVEKISTDKIETKVKTGGNISSHKGVNLPDAAVRLPAITEKDKKDIQFLLEENVD
CCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHCCCC
FIACSFVRKPSHIKEIRDFIQQYKETSPNIIAKIETMEAIENFQDICKEADGIMIARGDL
EEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPYQCIPLLQKIMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPIESVRTLRLVSEFAEHVKKDGPFAMKDVLQLLRESLDE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIDRICTIGPASNNKETLAQLIKNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKFLG
CCCCEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
DVQGPKIRLGEVKGEQITLQAGDSFILHTQSVTGSNTEASVDYEGIANDVKVGSRILIND
CCCCCEEEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHCCHHHCCCCEEEEEC
GEVELIVEKISTDKIETKVKTGGNISSHKGVNLPDAAVRLPAITEKDKKDIQFLLEENVD
CCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHCCCC
FIACSFVRKPSHIKEIRDFIQQYKETSPNIIAKIETMEAIENFQDICKEADGIMIARGDL
EEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPYQCIPLLQKIMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPIESVRTLRLVSEFAEHVKKDGPFAMKDVLQLLRESLDE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA